Time-dependent effect of deuterium depletion on tumor growth and metastasis

Abstract

<jats:p>Objective ― impact of different time regimens of deuterium depletion on progression of syngeneic grafted tumor in mice was investigated. Material and Methods ― Experiment was performed on 64 C57Bl/6 mice divided into two groups with substitution of regular water for deuterium depleted water: 30 days prior to tumor cell inoculation and sinse the 1st day of inoculation. Mice taking destilled mineralized water were considered as a control group. Half of the mice were injected melanoma B16 cells subcutaneously for assessment of survival rate and tumor growth inhibition index. The other mice were intravenously injected melanoma cells for estimation of lung hematogenous metastasis. Results ― Deuterium depletion began at the day of tumor inoculation did not change the parameters of survival rate (38.83±7.69 days vs. 41.33±6.22days in the control). Tumor growth inhibition was found only at initial stage of tumor progression. Number of melanoma lung metastasis did not differ from the control values (32.30±6.12 and 28.33±5.38. consequently). The group of mice with preliminary consumption of deuterium depleted water demonstrated significantly higher survival rate (70.10±16.20 days), tumor inhibition index, and attenuation of lung metastasis (6.0±1.20). Conclusion ― Our findings demonstrate that deuterium depletion exerts antitumor effect by both inhibition of tumor growth and metastasis, but development of the effect is time-dependent.</jats:p>

The underexplored links between cancer and the internal body climate: Implications for cancer prevention and treatment

“…Interesting, several in vitro studies suggested that water with a low deuterium concentration (<65 ppm) may inhibit cancer growth ( 221 – 229 ) and augments the inhibitory effect of paclitaxel ( 230 ). Anecdotal reports and clinical data from Europe suggest that lowering the levels of deuterium in the body by drinking deuterium depleted water (10-20 ppm) may improve quality of life and possibly survival of cancer patients ( 231 – 233 ).…”

Bilateral Shifts in Deuterium Supply Similarly Change Physiology of the Pituitary–Thyroid Axis, but Differentially Influence Na+/I− Symporter Production

“…Shifts in the deuterium body content have been shown to be easily achieved by the substitution of drinking water by deuterium-depleted or deuterium-enriched water, a simple and safe method [ 9 ]. The administration of deuterium-depleted water has been shown to improve the results of antitumor treatment and has been recommended as a supplemental treatment for chemotherapy [ 3 , 10 ]. As the beneficial effects of deuterium-depleted water resulted from the better function of mitochondrial oxidation, deuterium reduction has potentially become a promising tool in the treatment of metabolic disorders [ 4 , 7 ].…”

“…Lack of knowledge on deuterium function complicates the prediction and assessment of physiological changes induced by shifts in the deuterium/protium balance. It has been found that the consumption of deuterium-depleted water from the day of inoculation of tumor cells does not affect tumor growth and metastasis in mice, whereas preliminary deuterium depletion significantly reduces tumor growth and increases survival rate [ 10 ]. These results suggest that shifts in the deuterium body content affect metabolic patterns and alter organ physiology, and that development of physiological changes depends on the time and amplitude of the deuterium/protium disbalance.…”

Influence of the 2H/1H Isotope Composition of the Water Environment on the Probability of Denaturation Bubble Formation in a DNA Molecule

“…It is known that a change in the deuterium content of living systems affects their metabolic processes [2,3]. This applies to both elevated [4] and reduced deuterium concentrations relative to the natural level [5][6][7][8][9][10].…”