Time-dependent bladder tissue regeneration using bilayer bladder acellular matrix graft-silk fibroin scaffolds in a rat bladder augmentation model

Yang, Zhao; He, Yi; Guo, Jianhua; Wu, Jia-sheng; Zhou, Zhe; Zhang, Ming; Li, Wei; Zhou, Juan; Xiao, Dongdong; Wang, Zhong; Sun, Kang; Zhu, Yingke; Lu, Mengji

doi:10.1016/j.actbio.2015.05.032

Cited by 45 publications

(30 citation statements)

References 37 publications

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“…Bladder acellular matrix grafts (BAMG)-SF scaffold was synthesized as previously described [17]. Briefly, bladder tissues were harvested from 3-month-old pigs and rinsed with phosphate-buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 99%

The morphological regeneration and functional restoration of bladder defects by a novel scaffold and adipose-derived stem cells in a rat augmentation model

Wang

Xiao

et al. 2017

Stem Cell Res Ther

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BackgroundDue to the multilineage differentiation ability and paracrine role of adipose-derived stem cells (ASCs) for bladder defect repair, various scaffolds have been applied in combination with ASCs to promote bladder regeneration and restore bladder function. However, the low survival rate of ASCs and the difficulty of promoting bladder functional recovery are still unsolved. To explore these problems, we investigated the feasibility of a novel scaffold seeded with ASCs in a rat model of bladder augmentation.MethodsA novel autologous myofibroblast (AM)-silk fibroin (SF) scaffold was harvested after subcutaneously prefabricating the bladder acellular matrix grafts (BAMG) and SF by removing the BAMG. The AM-SF scaffolds were then seeded with ASCs (AM-SF-ASCs). Fifty percent supratrigonal cystectomies were performed followed by augmenting the cystectomized defects with AM-SF scaffolds or AM-SF-ASCs. The histological and functional assessments of bladders were performed 2, 4, and 12 weeks after surgery while the ASCs were tracked in vivo.ResultsFor bladder tissue regeneration, immunofluorescence analysis revealed that AM-SF-ASCs (the experimental group) promoted better morphological regeneration of the urothelium, vessels, bladder smooth muscle, and nerve than AM-SF scaffolds (the control group). Regarding functional restoration, the AM-SF-ASC group exhibited higher bladder compliance and relatively normal micturition pattern compared to the AM-SF group. In addition, a certain number of surviving ASCs could be found in vivo 12 weeks after implantation, and some of them had differentiated into smooth muscle cells.ConclusionsThe AM-SF scaffolds with ASCs could rapidly promote bladder morphological regeneration and improved bladder urinary function. In addition, the bag-shaped structure of the AM-SF scaffold can improve the survival of ASCs for at least 12 weeks. This strategy of AM-SF-ASCs has a potential to repair large-scale bladder defects in the clinic in the future.

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Section: Methodsmentioning

confidence: 99%

The morphological regeneration and functional restoration of bladder defects by a novel scaffold and adipose-derived stem cells in a rat augmentation model

Wang

Xiao

et al. 2017

Stem Cell Res Ther

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“…With the development of tissue engineering in recent years, the superiority of the bladder acellular matrix over other biological material has been demonstrated in a variety of experiments (Pokrywczynska et al 2015; Zhao et al 2015). The major advantages of the bladder acellular matrix are several: (1) The three-dimensional growth of the bladder acellular matrix is extremely similar to the original growth environment of the growth of the bladder.…”

Section: Discussionmentioning

confidence: 99%

In vitro culture of rat hair follicle stem cells on rabbit bladder acellular matrix

Wang

et al. 2016

SpringerPlus

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BackgroundThe aim of this work was to create a xenogeneic cell scaffold complex with rabbit bladder acellular matrix and rat hair follicle stem cells, to study the feasibility of construct tissue engineer bladder through biocompatibility of hair follicle stem cells and heterogeneous bladder acellular matrix.Material and MethodsNew Zealand rabbit bladder acellular matrix was prepared. Scanning electron microscope and Masson staining were used to analyse the acellular material. Two-steps precipitation method was used to place the third generation of hair follicle stem cells onto the surface of the bladder acellular matrix. The in vitro cell growth on the scaffold complex was regularly monitored through an inverted microscope. Cell growth curve was established and histological examination and scanning electron microscopic were used to analyse the progresses of the cell growth on the matrix material.ResultsThe prepared bladder acellular matrix was white, translucent and membranous. It possessed a fibrous network and collagen structure without any significant cell residues as displayed by the scanning electron microscope, and Masson staining. After 48 h of culture, observation by inverted microscope showed that the hair follicle stem cells grew well around the bladder acellular matrix. After 1 week of culture, scanning electron microscopy showed that the hair follicle stem cells spread and adhered on the surface of the scaffold.ConclusionsThe in vitro culture of rat hair follicle stem cells and the rabbit bladder acellular matrix possessed a good biocompatibility, which provides a good experiment support for hair follicle stem cells to repair the bladder defects disease.

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“…The rapid decline of neutrophils inside the regeneration area from 2 weeks is in accordance with the transient increase in white blood cells at 2 weeks, suggesting an acute inflammatory reaction without inflammatory dysregulation. The sequential chronic inflammation represented by CD68 + macrophages accelerated the degradation of SF fragments and was also recognized to be associated hyperplasia of the urothelial layer in our previous study [18]. Some studies have suggested that stem cells can attenuate tissue inflammatory responses [26].…”

Section: Discussionmentioning

confidence: 99%

“…The resulting matrix was washed with distilled water for 2 days at 4 °C and stored in 75% ethanol. The complete elimination of cellular nuclei from the BAMG was confirmed by histological evaluation and residual DNA content quantification in our previous work [18].…”

Section: Scaffold Preparationmentioning

confidence: 99%

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Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

Xiao¹,

Hu²,

Zhang³

et al. 2018

European Urology Supplements

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Time-dependent bladder tissue regeneration using bilayer bladder acellular matrix graft-silk fibroin scaffolds in a rat bladder augmentation model

Cited by 45 publications

References 37 publications

The morphological regeneration and functional restoration of bladder defects by a novel scaffold and adipose-derived stem cells in a rat augmentation model

The morphological regeneration and functional restoration of bladder defects by a novel scaffold and adipose-derived stem cells in a rat augmentation model

In vitro culture of rat hair follicle stem cells on rabbit bladder acellular matrix

Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

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