Time Dependency of Chemodiversity and Biosynthetic Pathways: An LC-MS Metabolomic Study of Marine-Sourced Penicillium

Roullier, Catherine; Bertrand, Samuel; Blanchet, Élodie; Peigné, Mathilde; Pont, Thibaut Robiou du; Guitton, Yann; Pouchus, Yves François; Grovel, Olivier

doi:10.3390/md14050103

Cited by 29 publications

(37 citation statements)

References 63 publications

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“…This difference in activity could also be due to difference in fungal biomass on the plugs as they had different times to grow between the samplings. This time-dependent difference in metabolism has been shown for different Penicillium species before (Khalil et al 2014 ; Roullier et al 2016 ).…”

Section: Discussionsupporting

confidence: 68%

Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity

et al. 2019

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During a research cruise in 2016, we isolated fungi from sediments, seawater, driftwood, fruiting bodies, and macroalgae using three different media to assess species richness and potential bioactivity of cultivable marine fungi in the High Arctic region. Ten stations from the Svalbard archipelago (73–80 °N, 18–31 °E) were investigated and 33 fungal isolates were obtained. These grouped into 22 operational taxonomic units (OTUs) using nuc rDNA internal transcribed spacer regions (ITS1-5.8S-ITS2 = ITS) with acut-off set at 98% similarity. The taxonomic analysis showed that 17 OTUs belonged to Ascomycota, one to Basidiomycota, two to Mucoromycota and two were fungal-like organisms. The nuc rDNA V1-V5 regions of 18S (18S) and D1-D3 regions of 28S (28S) were sequenced from representative isolates of each OTU for comparison to GenBank sequences. Isolates of Lulworthiales and Eurotiales were the most abundant, with seven isolates each. Among the 22 OTUs, nine represent potentially undescribed species based on low similarity to GenBank sequences and 10 isolates showed inhibitory activity against Gram-positive bacteria in an agar diffusion plug assay. These results show promise for the Arctic region as asource of novel marine fungi with the ability to produce bioactive secondary metabolites with antibacterial properties.

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Section: Discussionsupporting

confidence: 68%

Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity

et al. 2019

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“…227 A study which analysed two Penicillium strains at different time points throughout the culture period by HPLC-DAD-HRMS indicated that a greater chemodiversity of metabolites may be obtained from the one strain by performing extractions at different times throughout the culture period including early and late growth phases. 228 Studies on the effect of light on the biosynthesis of the anticancer polyketide 1403C (haloroquinone, SZ-685C) in Halorosellinia sp., indicated that light signicantly increased production of this metabolite (74%) over production in the dark. 229 Comparison of the secondary metabolite prole of a monoculture of Aspergillus avipes with that of a strain cocultured with a Streptomyces strain isolated from the same marine sediment, indicated that physical contact with the bacterium induced production of six cytotoxic cytochalasans (such as aspochalasin P) in the fungus.…”

Section: Marine-sourced Bacteriamentioning

confidence: 99%

Marine natural products

et al. 2018

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Covering: 2016. Previous review: Nat. Prod. Rep., 2017, 34, 235-294This review covers the literature published in 2016 for marine natural products (MNPs), with 757 citations (643 for the period January to December 2016) referring to compounds isolated from marine microorganisms and phytoplankton, green, brown and red algae, sponges, cnidarians, bryozoans, molluscs, tunicates, echinoderms, mangroves and other intertidal plants and microorganisms. The emphasis is on new compounds (1277 in 432 papers for 2016), together with the relevant biological activities, source organisms and country of origin. Reviews, biosynthetic studies, first syntheses, and syntheses that led to the revision of structures or stereochemistries, have been included.

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“…The agar samples contained fungal mycelium and/or dinoflagellates in a biofilm and the liquid supernatant contained only P. lima when inoculated (fungal spores traces were observed). To obtain a clear view of metabolites present in both types of extracts, they were profiled by LC-HRMS [41]. For sake of clarity, single strain P. lima culture corresponds to the dinoflagellates with (xenic) or without (axenic) their associated bacteria depending on the presence of antibiotics.…”

Section: Specific Microscale Marine Environment Was Designed For Co-culturementioning

confidence: 99%

Deciphering microbiome impacts on fungal-microalgal interaction in a marine environment using metabolomics

Berry

Briand

Bagot

et al. 2021

Preprint

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The comprehension of microbial interactions is one of the key challenges in microbial ecology. The present study focuses on studying the chemical interaction between the toxic dinoflagellate Prorocentrum lima PL4V strain and associated fungal strains (two Penicillium sp. strains and three Aspergillus sp) among which the Aspergillus pseudoglaucus strain MMS1589 was selected for further co-culture experiment. Such rarely studied interaction (fungal-microalgal) was explored in axenic and non-axenic conditions, in a dedicated microscale marine environment (hybrid solid/liquid conditions), to delineate specialized metabolome alteration in relation to the P. lima and A. pseudoglaucus co-culture in regard to the presence of their associated bacteria. Such alteration was monitored by high-performance liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). In-depth analysis of the resulting data highlighted (1) the chemical modification associated to fungal-microalgal co-culture, and (2) the impact of associated bacteria in microalgal resilience to fungal interaction. Even if only a very low number of highlighted metabolites were fully characterised due to the poor chemical investigation of the studied species, a clear co-culture induction of the dinoflagellate toxins okadaic acid and dinophysistoxin 1 was observed. Such results highlight the importance to consider microalgal microbiome to study parameters regulating toxin production. Finally, a microscopic observation showed an unusual physical interaction between the fungal mycelium and the dinoflagellates.

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Time Dependency of Chemodiversity and Biosynthetic Pathways: An LC-MS Metabolomic Study of Marine-Sourced Penicillium

Cited by 29 publications

References 63 publications

Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity

Cultivable marine fungi from the Arctic Archipelago of Svalbard and their antibacterial activity

Marine natural products

Deciphering microbiome impacts on fungal-microalgal interaction in a marine environment using metabolomics

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