Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol

Genovese, Griselda; Regueira, Mariana; Piazza, Yanina Grisel; Towle, David W.; Maggese, M.C.; Nostro, Fabiana Laura Lo

doi:10.1016/j.aquatox.2012.02.005

Cited by 25 publications

(13 citation statements)

References 78 publications

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“…Both Pearson correlation and RDA showed that estrogenic compounds such as NP and 4-t-OP in the rivers could have contributed to the induction of Vtg and ERα mRNA expression in mosquitofish. Previous laboratory studies have shown that NP and 4-t-OP have the potential to induce Vtg in males, form intersex gonads in males, and cause other signs of reproductive impairment 24 25 . In addition, NP and 4-t-OP have been reported to be the primary cause of feminized effects downstream of industrial wastewater discharges 26 .…”

Section: Discussionmentioning

confidence: 99%

Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters

Huang

Liu

Chen

et al. 2016

Sci Rep

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Municipal wastewaters have been known to contain various estrogens and androgens. Little is known about the joint action of these chemicals from wastewaters on fishes in the aquatic environment. The objectives of this study were to investigate the estrogenic and/or androgenic effects in wild mosquitofish (Gambusia affinis) of two effluent-impacted rivers in South China by determining morphological changes and hepatic mRNA expression levels of relevant genes such as vitellogenin (Vtg), estrogen receptor (ERα) and androgen receptors (ARα and ARβ), and to assess the linkages of those morphological changes and hepatic mRNA expression levels to the chemical concentrations measured by in vitro bioassays and chemical analysis. The results showed a significant induction of Vtg and ERα mRNA in the livers of the males and a gonopodium-like anal fin in the females collected at the majority of sites. Redundancy analysis and Pearson correlation analysis showed that the chemical concentrations obtained by in vitro bioassays and chemical analysis had significant correlations with some of the endpoints for the estrogenic and/or androgenic effects in mosquitofish. The findings from this study indicate that the estrogens and androgens present in the two rivers could cause the observed estrogenic and androgenic effects in mosquitofish.

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Section: Discussionmentioning

confidence: 99%

Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters

Huang

Liu

Chen

et al. 2016

Sci Rep

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“…Previously published partial sequences for C. dimerus were used to design specific primers of zona pellucida protein B (zpB) (Genovese et al, 2011), vitellogenin Ab (vtgAb) (Genovese et al, 2012), β subunit of luteinizing hormone (β-lh) and β subunit of follicle stimulating hormone (β-fsh) (Pandolfi et al 2009b, Di Yorio et al 2015, gonadal aromatase (cyp19a1A) (Ramallo et al unpublished) and β-actin (GenBank, NCBI) using Primer-BLAST software (Table 1).…”

Section: Semiquantitative Gene Expression Analysismentioning

confidence: 99%

“…The acará, Cichlasoma dimerus (Heckel, 1840), is a South American cichlid fish that has recently emerged as a well-established laboratory model for the study of reproduction, neuroendocrinology and behaviour (see Pandolfi et al 2009a andRamallo et al 2014 for review) and also for toxicological studies (Rey Vázquez et al 2009;Genovese et al 2012Genovese et al , 2014Da Cuña et al 2013Piazza et al 2015;Meijide et al 2016). This species exhibits a hierarchical social system established and sustained through agonistic interactions.…”

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confidence: 99%

Dynamics of ovarian maturation throughout the reproductive cycle of the Neotropical cichlid fishCichlasomadimerus(Teleostei, Cichliformes)

et al. 2017

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In this study, we analyzed gene expression profiles, plasma steroids concentrations, and gonadal morphology throughout the reproductive cycle of female Cichlasoma dimerus (Heckel, 1840), a monogamous cichlid fish exhibiting social hierarchies. Fish were analyzed at six phases encompassing their annual cycle, namely resting (during the nonreproductive period), prespawning, 30 h post spawning, 4 days post spawning, 10 days post spawning, and subordinate (during the reproductive period). The histological and histomorphometric analysis showed that C. dimerus exhibits asynchronous ovarian development. Similar to resting females, subordinate females showed low gonadosomatic index, reduced expression levels of vitellogenin (vtgAb), zona pellucida (zpB), gonadal aromatase (cyp19a1A), and low concentrations of plasma sex steroids, thus indicating that social intimidation by dominant conspecifics elicited reproductive arrest. In reproductively active females, a direct positive correlation between plasma estradiol, vtgAb expression, percentage of late vitellogenic oocytes, and gonadosomatic index was observed. These parameters were maximal at the prespawning phase, decreased at 30 h post spawning and 4 days post spawning, and then reached a peak at 10 days post spawning. Our results indicate that female C. dimerus become spawning capable after 10 days post spawning, coincidently with the shortest time interval between successive spawns recorded in captivity.

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“…Vitellogenin is typically synthesized in female livers through endogenous 17-β-oestradiol (E2) regulation, transported via the blood stream to the ovaries, and absorbed by maturing oocyte s (Arukwe & Goksøyr 2003). Concentrations of vitellogenin (VTG), which are typically very low or absent in male and juvenile fi sh, can increase with xenoestrogen exposure, thereby providing a sensitive indicator of oestrogenic EDC contamination in aquatic environments (Arukwe & Goksøyr 2003;Maltais & Roy 2009;Genovese et al 2012). Vitellogenin levels have been studied in fi sh blood and mucus with different analytical techniques (Moncaut et al 2003;Arukwe & Røe 2008;Rey-Vázquez et al 2009;Pollino et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluents

Bahamonde¹,

Berrocal

Barra

et al. 2019

Gayana (Concepc.)

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Time-course recovery of estrogen-responsive genes of a cichlid fish exposed to waterborne octylphenol

Cited by 25 publications

References 78 publications

Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters

Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters

Dynamics of ovarian maturation throughout the reproductive cycle of the Neotropical cichlid fishCichlasomadimerus(Teleostei, Cichliformes)

Mucus phosphoproteins as an indirect measure of endocrine disruption in native small-bodied freshwater fish, exposed to wastewater treatment plant and pulp and paper mill effluents

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