Time course proteomic profiling of human myocardial infarction plasma samples: An approach to new biomarker discovery

Silbiger, Vivian Nogueira; Luchessi, André Ducati; Hirata, Rosário Dominguez Crespo; Neto, Lídio Gonçãlves Lima; Pastorelli, Carla Prisinzano; Ueda, Eric; Santos, Elizabete Silva dos; Pereira, Marcos Paulo Torres; Ramos, Rui Fernando; Sampaio, Marcelo Ferraz; Armaganijan, Dikran; Paik, Seung Hoon; Murata, Yoko; Ooi, Guck T.; Ferguson, Earl W.; Hirata, Mário Hiroyuki

doi:10.1016/j.cca.2011.02.030

Cited by 20 publications

(13 citation statements)

References 31 publications

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“…In the last decade, there have been several efforts to dissect out cardiac mRNA and proteome in a wide array of cardiovascular diseases (McGregor and Dunn, 2006) using different animal models or plasma samples collected from human patients (Seenarain et al, 2010; Haas et al, 2011; Silbiger et al, 2011; Drastichova et al, 2012; Chowdhury et al, 2013; Marshall et al, 2014; Petriz and Franco, 2014). While novel biomarkers were identified from some of these studies (Haas et al, 2011; Silbiger et al, 2011; Chowdhury et al, 2013), authors reported general alterations in the cardiac transcriptome and proteome profile that suggested changes in Ca2+ handling proteins (Seenarain et al, 2010), energy metabolism proteins (Jin et al, 2006; Meng et al, 2009), and mediators of apoptotic signaling (Drastichova et al, 2012; Marshall et al, 2014; Petriz and Franco, 2014). …”

Section: Discussionmentioning

confidence: 99%

Comparative mRNA and MicroRNA Profiling during Acute Myocardial Infarction Induced by Coronary Occlusion and Ablation Radio-Frequency Currents

et al. 2016

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The ligation of the left anterior descending coronary artery is the most commonly used experimental model to induce myocardial infarction (MI) in rodents. A high mortality in the acute phase and the heterogeneity of the size of the MI obtained are drawbacks recognized in this model. In an attempt to solve the problem, our group recently developed a new MI experimental model which is based on application of myocardial ablation radio-frequency currents (AB-RF) that yielded MI with homogeneous sizes and significantly reduce acute mortality. In addition, cardiac structural, and functional changes aroused by AB-RF were similar to those seen in animals with MI induced by coronary artery ligation. Herein, we compared mRNA expression of genes that govern post-MI milieu in occlusion and ablation models. We analyzed 48 mRNAs expressions of nine different signal transduction pathways (cell survival and metabolism signs, matrix extracellular, cell cycle, oxidative stress, apoptosis, calcium signaling, hypertrophy markers, angiogenesis, and inflammation) in rat left ventricle 1 week after MI generated by both coronary occlusion and AB-RF. Furthermore, high-throughput miRNA analysis was also assessed in both MI procedures. Interestingly, mRNA expression levels and miRNA expressions showed strong similarities between both models after MI, with few specificities in each model, activating similar signal transduction pathways. To our knowledge, this is the first comparison of genomic alterations of mRNA and miRNA contents after two different MI procedures and identifies key signaling regulators modulating the pathophysiology of these two models that might culminate in heart failure. Furthermore, these analyses may contribute with the current knowledge concerning transcriptional and post-transcriptional changes of AB-RF protocol, arising as an alternative and effective MI method that reproduces most changes seem in coronary occlusion.

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Section: Discussionmentioning

confidence: 99%

Comparative mRNA and MicroRNA Profiling during Acute Myocardial Infarction Induced by Coronary Occlusion and Ablation Radio-Frequency Currents

et al. 2016

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“…Time course proteomic profiling has also identified multiple proteins differently expressed in the first 48 h after AMI. 298 Activation of the compliment system has also been shown by a recent proteomic analysis of plasma from patients with AMI. 299 Decreased plasma levels of soluble tumor necrosis factorlike weak inducer of apoptosis (sTWEAK) has been identified by using proteomic approach in patients with carotid stenosis, CAD, HF, PAD and abdominal aortic aneurysm (AAA).…”

Section: Proteomics and Metabolomicsmentioning

confidence: 92%

The Path to Personalized Cardiovascular Medicine

Marı́n-Garcı́a

2014

Post-Genomic Cardiology

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“…Both known biomarkers and novel candidates were discovered . A similar high‐abundance protein depletion strategy was used to discover biomarkers in patients diagnosed with an MI . More recently, a more powerful depletion strategy, IgY14‐supermix tandem depletion method, was utilized to seek predictive markers for near‐term MI .…”

Section: Quantitative Global Proteomics Methodsmentioning

confidence: 99%

Quantitative proteomics in cardiovascular research: Global and targeted strategies

Shen

Young

Canty

et al. 2014

Proteomics Clinical Apps

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Extensive technical advances in the past decade have substantially expanded quantitative proteomics in cardiovascular research. This has great promise for elucidating the mechanisms of cardiovascular diseases (CVD) and the discovery of cardiac biomarkers used for diagnosis and treatment evaluation. Global and targeted proteomics are the two major avenues of quantitative proteomics. While global approaches enable unbiased discovery of altered proteins via relative quantification at the proteome level, targeted techniques provide higher sensitivity and accuracy, and are capable of multiplexed absolute quantification in numerous clinical/biological samples. While promising, technical challenges need to be overcome to enable full utilization of these techniques in cardiovascular medicine. Here we discuss recent advances in quantitative proteomics and summarize applications in cardiovascular research with an emphasis on biomarker discovery and elucidating molecular mechanisms of disease. We propose the integration of global and targeted strategies as a high-throughput pipeline for cardiovascular proteomics. Targeted approaches enable rapid, extensive validation of biomarker candidates discovered by global proteomics. These approaches provide a promising alternative to immunoassays and other low-throughput means currently used for limited validation.

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Time course proteomic profiling of human myocardial infarction plasma samples: An approach to new biomarker discovery

Cited by 20 publications

References 31 publications

Comparative mRNA and MicroRNA Profiling during Acute Myocardial Infarction Induced by Coronary Occlusion and Ablation Radio-Frequency Currents

Comparative mRNA and MicroRNA Profiling during Acute Myocardial Infarction Induced by Coronary Occlusion and Ablation Radio-Frequency Currents

The Path to Personalized Cardiovascular Medicine

Quantitative proteomics in cardiovascular research: Global and targeted strategies

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