Time Course of the Secondary Deposition of Incrusting Materials on Bordered Pit Membranes in Cryptomeria Japonica

Abstract

Bordered pit membranes of Cryptomeria japonica were examined successively from the outermost sapwood to the heartwood by scanning electron microscopy and by ultraviolet microspectrophotometry in an attempt to evaluate the time course of the secondary deposition of incrusting materials and to gain clues to their chemical composition. Scanning electron microscopy revealed that the bordered pit membranes were covered by incrusting materials from the middle layer of the sapwood to the heartwood. Both the amount and the appearance of the deposited incrusting materials differed among four regions of the wood, namely, the middle to inner layer of the sapwood, the innermost layer of the sapwood, the intermediate wood and the heartwood. From our results it appears that, in C. japonica, incrusting materials are deposited on bordered pit membranes by stages over several years. Apparent absorption of ultraviolet light by the bordered pit membranes was detected in the analysis of the innermost layer of the sapwood, the intermediate wood and the heartwood. The incrusting materials deposited in these zones were probably phenolic compounds. However, differences in the manner and extent of the absorption of ultraviolet light were found between these three regions of the wood. The results of microspectrophotometric analysis also suggested the phased deposition of incrusting materials at the bordered pit membranes of C. japonica.

Isolation of a novel cell wall architecture mutant of rice with defective Arabidopsis COBL4 ortholog BC1 required for regulated deposition of secondary cell wall components

“…Sections (1 m) were cut with a glass knife on an ultramicrotome (Ultracut N; Reichert), placed on quartz slides, mounted in glycerin with quartz coverslips, and observed with an UV microscope (MPM800; Carl Zeiss, Oberkochen, Germany). UV micrographs were taken on conventional black-andwhite Wlm (Neopan 100 Across; FujiWlm, Tokyo Japan) at 280 nm with a 5-nm wavelength range (Sano and Nakada 1998;Takabe 2002;Yoshida et al 2005;Watanabe et al 2006).…”

Temporal and spatial diversities of the immunolabeling of mannan and xylan polysaccharides in differentiating earlywood ray cells and pits of Cryptomeria japonica

“…Additionally, Putoczki et al (2008) showed abundant deposition of unesteriWed and methyl-esteriWed pectic polysaccharides in bordered pit membranes of radiata pine. Although several studies have reported the presence of phenolic compounds in bordered pit membranes of softwood (Bauch et al 1968;Bauch and Berndt 1973;Sano and Nakada 1998), the presence of lignin in pit membranes in softwoods is still not clear.…”

Application of Scanning UV Microspectrophotometry to Localise Lignins and Phenolic Extractives in Plant Cell Walls

“…Bland and Hillis 1969;Hillis et al 1976;Imagawa and Fukazawa 1978;Kleist and Bauch 2001) and (v) the chemical composition of pit membranes of gymnosperms (e.g. Bauch and Berndt 1973;Sano and Nakada 1998). All these investigations were performed by UV microscopic point measurements using a spot size of l µm 2 of cell wall area or two dimensional UV scanning profiles across the cell wall width as shown by Bauch et al (1976).…”