Time course of serum malondialdehyde concentrations as a marker of oxidative stress in experimental canine osteotomies fixed by two different techniques

Paskalev, M.

doi:10.1007/s00580-008-0796-1

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…This enzyme triggers the mineralization of the osteoid by increasing the local concentration of calcium phosphate. Alkaline phosphatase is an indicator for bone formation, and higher serum level of this enzyme shows faster maturation and more activity of osteoblasts [ 28 ]. The higher alkaline phosphatase levels in the PDE 300 group and the osteocare group 10 days after surgery indicate greater osteoblast activity, and faster healing.…”

Section: Discussionmentioning

confidence: 99%

The effect of aqueous extract of Prunus dulcis on tibial bone healing in the rabbit

et al. 2021

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Background Bone fractures are medical emergencies that require prompt intervention to help return bone to its normal function. Various methods and treatments have been utilized to increase the speed and efficiency of bone repair. This study aimed to investigate the treatment effects of Prunus dulcis aqueous extract on tibial bone healing in rabbits. Methods All animals were distributed in five groups with six rats in each group, including the sham group, the control group in which tibial lesion was made and received distilled water, treatment groups with 150 mg kg−1, 300 mg kg−1 doses of Prunus dulcis extract, and osteocare treated group. Biochemical blood factors including calcium, phosphorus, and alkaline phosphatase (on days 0, 10, 30, and 50), biomarkers of oxidative stress such as GPx, CAT, and MDA (on days 10 and 30), radiological evaluation, histopathological parameters, and osteocalcin immunohistochemical expression were assessed. Results The data showed calcium levels in the treatment groups increased significantly from day 10 to day 50, respectively, and blood phosphorus levels decreased from day 10 to day 50 in the treatment groups. Alkaline phosphatase initially increased and then decreased in treatment groups. In the treatment groups, GPx and CAT levels significantly increased, and the serum amount of MDA reduced. The best antioxidant results were related to the extract-treated group with a higher dose. Radiographic score was significantly higher in the treatment groups than the control group on day 30. Based on the pathological findings, the healing occurred faster in the extract-treated group with a higher dose. Osteocalcin expression was significantly higher in the control group than that in the treatment groups. Conclusions Treatment with Prunus dulcis extract with a dosage of 300 mg/kg accelerated tibial bone healing in rabbits. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

The effect of aqueous extract of Prunus dulcis on tibial bone healing in the rabbit

et al. 2021

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“…Human and experimental studies have reported that there is an increase in the production of free radicals by macrophages, polymorphonuclear leukocytes and osteoclasts during fi rst month after a fracture has been sustained. Oxidative stress clearly prolongs the infl ammatory and repair periods of fracture healing (7)(8)(9)(10).…”

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confidence: 99%

TNF-α, IL-1β, and oxidative stress during fracture healing with or without ankaferd

Amanvermez¹,

Günay²,

Pişkin³

et al. 2013

BLL

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Aims: Whether ankaferd blood stopper (ABS) has a negative or positive effect on bone union during fracture healing is unknown. The purpose of this study was to evaluate the serum changes of oxidative stress markers, Tumor Necrosis Factor-α (TNF-α), Interleukin-1β (IL-1β), and Interleukin-10 (IL-10) during fracture healing process with or without ABS application to bone fracture. Material and methods: Eight rats were used as a control group (1) that was not subject to fracture. The remaining 48 rats were divided into six groups, 8 rats in each. The femoral shaft fracture was produced by cutting with bone-scissors. One ml of ABS was applied on the fracture region in groups 3 (7th day), 5 (21st day), and 7 (45th day) or saline instead of ABS on the fracture regions in groups 2 (7th day), 4 (21st day), and 6 (45th day). Radiographs and above parameters were examined on post-fracture days 7, 21, and 45. Results: Malondialdehyde and protein carbonyls were measured in high levels in the groups 2 and 4 with respect to control. Their levels did not change statistically in the experimental groups after ABS application. The values of TNF-α and IL-1β were elevated on 7th post-fracture day according to control, but were lower (by 11.86 % and 44.48 %) in the group 3 treated with ABS comparing to group 2. Radiographic examination indicated a low callus formation on fracture union in the femoral fractures of groups 3 and 5 treated with ABS. Conclusion:The present fi ndings may suggest that ABS application seems to be ineffective on fracture union in early fracture healing period, except for bleeding control (Tab. 2, Fig. 3, Ref. 26). Full Text in PDF www.elis.sk.

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“…In humans and animals, alkaline phosphatase isoenzymes originate from the liver, intestines, placenta, kidneys, and bones [58]. Our attention was focused on bone alkaline phosphatase (BALP) as it is considered to be more specific [59]. An increase in BALP activity was observed in the second week after osteosynthesis compared to that at the baseline level.…”

Section: Discussionmentioning

confidence: 99%

Effects of Erythropoietin-Promoted Fracture Healing on Bone Turnover Markers in Cats

Vasileva,

Chaprazov,

Milanova

2024

JFB

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In orthopaedics, erythropoietin (EPO) is applied in the preoperative management of anaemic patients, but also as a stimulating factor to assist bone regeneration due to its angiogenic and osteoinductive potential. Since orthopaedists mainly rely on their clinical experience to assess bone healing, additional and more objective methods such as studying the dynamics of bone markers are needed. Therefore, the aim of this study was to investigate the plasma activity of bone-specific alkaline phosphatase (BALP), the N-terminal propeptide of type I collagen (PINP), the C-terminal telopeptide of type I collagen (CTX), and deoxypyridinoline (DPD) during the first 2 months of healing of comminuted fractures in cats, either non-stimulated or locally stimulated with recombinant human erythropoietin (rhEPO). The study included twelve cats of mixed breeds, aged 7.2 ± 4 months, weighing 2.11 ± 1.1 kg, with comminuted diaphyseal fractures of the femur. Surgical treatment with plate osteosynthesis was performed in all animals. The cats were randomly divided into two groups—a control (n = 6) and an EPO group (n = 6). The locally applied EPO leads to the increased activity of bone formation markers (BALP and PINP) during the second week after the osteosynthesis, preceding the peaks in the control group by two weeks. The studied bone resorption markers (DPD, CTX) varied insignificantly during the studied period. In conclusion, erythropoietin could serve as a promoter of bone healing in comminuted fractures in cats.

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Time course of serum malondialdehyde concentrations as a marker of oxidative stress in experimental canine osteotomies fixed by two different techniques

Cited by 4 publications

References 14 publications

The effect of aqueous extract of Prunus dulcis on tibial bone healing in the rabbit

The effect of aqueous extract of Prunus dulcis on tibial bone healing in the rabbit

TNF-α, IL-1β, and oxidative stress during fracture healing with or without ankaferd

Effects of Erythropoietin-Promoted Fracture Healing on Bone Turnover Markers in Cats

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