Time Course of Serum Estrogen and Luteinizing Hormone (LH) Concentrations During the Menstrual Cycle of the Rhesus Monkey

Hotchkiss, J.; Atkinson, Linda E.; Knobil, E.

doi:10.1210/endo-89-1-177

Cited by 616 publications

(170 citation statements)

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“…Progesterone and oestradiol-17β concentrations were determined according to the method of Hotchkiss et al [18] modified by Kotwica [27], those of A 4 and E 1 as described by Dziadkowiec et al [9] and those of T according to Kotwica and Williams [28]. Antibodies against E 1 (BSz/88/706) showed the following cross-reactivities (oestrone 100%): oestradiol-17β-propionate (0.00%), 5α-androstane-3,17 dione (0.02%), androstenedione (0.01%), epiandrosterone (0.01%), androsterone (0.00%), progesterone (0.00%), testosterone (0.00%), 5β-androstane-3,17-dione (0.00%), 5β-androstane-3α, 17β-diol (0.00%), 4 androstane-11β-ol 3,17-dione (0.00%), 5α-pregnan-3α-ol-20-one (0.00%), 5α-pregnan-3β-ol-20-one (0.00%), 4-pregnen-20α-ol-3-one (0.00%), 4-pregnen-20β-ol-3-one (0.00%).…”

Section: Radioimmunoassays Of Steroid Hormonesmentioning

confidence: 99%

The physiological role of ?-endorphin in porcine ovarian follicles

et al. 2000

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-β-Endorphin-like immunoreactivity (β-END-LI) was measured by radioimmunoassay in porcine ovarian follicular fluid (FF) from small, medium and large follicles throughout the oestrous cycle. The concentration of β-END-LI in FF from small follicles collected on days 1-5 of the cycle was at least tenfold higher than in the fluid from any other follicles independently from their size and the period of the cycle. The level of β-END-LI in small follicles on days 6-10 was drastically decreased. Subsequently, on days 11-16 its concentration was enhanced and reduced again in preovulatory period of the cycle. Concentrations of β-END-LI in FF from medium follicles were relatively equal throughout the cycle (days 6-21). No significant differences in β-END-LI levels were found between small, medium and large follicles from days 17-21. However, β-END-LI concentrations in medium follicles on days 11-13 and 14-16 were statistically lower than those in small follicles. Moreover, the effects of FSH, prolactin (PRL), progesterone (P 4 ), testosterone (T) and 17 β-oestradiol (E 2 ) on β-END-LI release by granulosa cells (GCs) from large follicles and, on the other hand, the effects of the opioid agonist FK 33-824 alone or in combination with FSH, PRL or naloxone (NAL) on follicular steroidogenesis were studied. FSH drastically increased β-END-LI output in a dose-dependent fashion. This stimulatory effect of the gonadotrophin was inhibited by the highest dose of P 4 (10 -5 M). The effect of PRL and the steroids added to the cultures on β-END-LI release was negligible. FSH-or PRL-induced P 4 secretion by GCs was essentially abolished by both FK 33-824 and NAL. However, androstenedione (A 4 ) and testosterone output by the cells was greatly potentiated by FK 33-824. In the presence of NAL, FSH or PRL, A 4 release stimulated by FK 33-824 was suppressed to the basal level. Secretion of E 2 was completely free from the influence of FK 33-824 or NAL; only oestrone (E 1 ) output was modulated by them in cultures where FSH or PRL was present. In conclusion, FSH appears to be the key regulator of β-END-LI secretion by porcine granulosa cells. Moreover, steroidogenesis in pig granulosa cells is modulated by opioid peptides acting both alone and by way of interaction with FSH or PRL.opioid peptides / β-endorphin / porcine granulosa cells / steroid secretion Reprod. Nutr. Dev. 40 (2000) 63-75 63

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Section: Radioimmunoassays Of Steroid Hormonesmentioning

confidence: 99%

The physiological role of ?-endorphin in porcine ovarian follicles

et al. 2000

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“…The increased motility in vitro of the uterovarian ligament of the rabbit at ovulation is consistent with these observations and may reflect increased activity in vivo at the time of ovulation. Acute oestrogen withdrawal is associated with increased motility of the rabbit mesotubarium superius (Halbert & Conrad, 1975), and oestrogens have been shown to decline in vivo at the time of ovulation in the rabbit and other animals (Baird & Guevara, 1969;Hilliard & Eaton, 1971 ;Hotchkiss, Atkinson & Knobil, 1971), but the mechanisms regulating mesovarian ligament motility remain to be determined.…”

Section: Discussionmentioning

confidence: 99%

The motility in vitro of the rabbit uterovarian ligament

Fredericks¹,

Azzam²,

Hafez³

1977

Reproduction

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“…Oestradiol levels were measured by radioimmunoassay (Hotchkiss, Atkinson and Knobil 1971) . Diethyl ether extracts of 2 ml plasma specimens were assayed after chromatography on small Sephadex LH-20 columns, using iso-octane/benzene/methanol (62/20/18) as the eluting agent.…”

Section: Methodsmentioning

confidence: 99%