Time Course of Postnatal Distribution of Doublecortin Immunoreactive Developing/Maturing Neurons in the Somatosensory Cortex and Hippocampal CA1 Region of C57BL/6 Mice

Yoo, Dae Young; Yoo, Ki‐Yeon; Choi, Ji Won; Kim, Woosuk; Lee, Choong Hyun; Choi, Jung Hoon; Park, Jeong Ho; Won, Moo‐Ho; Hwang, In Koo

doi:10.1007/s10571-011-9670-9

Cited by 9 publications

(12 citation statements)

References 38 publications

(42 reference statements)

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“…In both genotypes we observed a similar number of DCX + immature neurons in the CA1. Consistent with previous observations (Gong et al, 2010; Inta et al, 2008; Yoo et al, 2011), the number of DCX + cells was low and steeply declined with age (Fig. 9).…”

Section: Resultssupporting

confidence: 93%

Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice

et al. 2014

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The activation of epidermal growth factor receptor (EGFR) affects multiple aspects of neural precursor behaviour, including proliferation and migration. Telencephalic precursors acquire EGF responsiveness and upregulate EGFR expression at late stages of development. The events regulating this process and its significance are still unclear. We here show that in the developing and postnatal hippocampus (HP), growth/differentiation factor (GDF) 15 and EGFR are co-expressed in primitive precursors as well as in more differentiated cells. We also provide evidence that GDF15 promotes responsiveness to EGF and EGFR expression in hippocampal precursors through a mechanism that requires active CXC chemokine receptor (CXCR) 4. Besides EGFR expression, GDF15 ablation also leads to decreased proliferation and migration. In particular, lack of GDF15 impairs both processes in the cornu ammonis (CA) 1 and only proliferation in the dentate gyrus (DG). Importantly, migration and proliferation in the mutant HP were altered only perinatally, when EGFR expression was also affected. These data suggest that GDF15 regulates migration and proliferation by promoting EGFR signalling in the perinatal HP and represent a first description of a functional role for GDF15 in the developing telencephalon.

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Section: Resultssupporting

confidence: 93%

Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice

et al. 2014

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“…The tissue sections were selected between )1.46 and )2.46 mm posterior to the bregma in reference to a mouse atlas [35] for each animal. pCREB immunohistochemical staining was performed according to a previous study using paraffin-embedded block [36]. Briefly, the sections were placed in 400-mL jars filled with citrate buffer (pH 6.0) and heated in a microwave oven (Optiquick Compact, Moulinex) operating at a frequency of 2.45 GHz and 800 W power setting.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Melatonin improves d‐galactose‐induced aging effects on behavior, neurogenesis, and lipid peroxidation in the mouse dentate gyrus via increasing pCREB expression

Yoo

Kim

Lee

et al. 2011

Journal of Pineal Research

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113

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Melatonin (N-acetyl-5-methoxytryptamine) has multiple functions. In this study, we investigated the effects of melatonin on memory, cell proliferation, and neuroblast differentiation in the dentate gyrus of a mouse model of D-galactose-induced aging. D-galactose was subcutaneously administered to 7-wk-old mice for 10 wk, and age-matched mice were used as controls. Seven weeks after D-galactose administration, vehicle (water) or melatonin (6 mg/L in water) was administered ad libitum to the mice for 3 wk. The administration of D-galactose significantly increased the escape latency compared with that in the control mice on days 1-3. In addition, cells in the subgranular zone and in the granule cell layer of the dentate gyrus showed severe damage (cytoplasmic condensation) in the D-galactose-treated mice. However, melatonin supplementation to these mice for 3 wk significantly ameliorated the D-galactose-induced increase in escape latency and neuronal damage compared with the vehicle-treated group. The administration of melatonin also significantly restored the D-galactose-induced reduction of proliferating cells (Ki67-positive cells) and differentiating neuroblasts (doublecortin-positive neuroblasts) in the dentate gyrus. Furthermore, the administration of melatonin significantly increased Ser133-phosphorylated cyclic AMP response element binding protein in the dentate gyrus. The administration of melatonin significantly reduced D-galactose-induced lipid peroxidation in the dentate gyrus. These results suggest that melatonin may be helpful in reducing age-related phenomena in the brain.

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“…The hippocampus is one of the most intensely studied brain regions given its function in learning and memory, providing that it is a site of neurogenesis and that it has a unique cellular organization and development (1). In the mouse hippocampus, a small number (15%) of granule cells are generated during embryogenesis, while the others are produced within 2 weeks after birth (2–4). Although we demonstrated in previous studies that the structural lamination of the granule cell layer is accomplished by 2 weeks after birth (4,5), maturation was observed at 3 weeks after birth (6).…”

Section: Introductionmentioning

confidence: 99%

“…The day of birth was considered day 0. Litters were culled to a maximum of 7 pups at the day of birth, as described in previous studies (4–6). From any one litter, a maximum of two animals were taken for each age group, ensuring that animals of a given age originated from at least four different litters.…”

Section: Introductionmentioning

confidence: 99%

“…Animals from each age group as well as COX-2 KO and WT mice of 12 weeks of age were euthanized with a urethane injection (1.5 g/kg body weight, intraperitoneally, Sigma; Merck KGaA, Darmstadt, Germany). While the brains from each age group were fixed for 24 h in 10% neutral buffered formalin at 25°C, COX-2 KO and WT mice were perfused transcardially with 0.1 M PBS (pH 7.4) followed by 4% paraformaldehyde in a 0.1 M phosphate-buffer (pH 7.4), as described previously (4–6). Subsequently, their brains were dissected and post-fixed for 12 h in the same fixative; subsequently, they were dehydrated with graded concentrations of alcohol to be embedded in paraffin.…”

Section: Introductionmentioning

confidence: 99%

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Postnatal changes in constitutive cyclooxygenase‑2 expression in the mice hippocampus and its function in synaptic plasticity

et al. 2019

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Although the expression of cyclooxygenase-2 (COX-2) is closely associated with inflammation in the brain, it is constitutively expressed in the brain, and its expression is regulated by synaptic activity. The present study investigated postnatal expression of COX-2 in the hippocampus in C57BL/6 mice at postnatal days (P) 1, 7, 14, 28, and 56. In addition, the presented study examined the effects of COX-2 on synaptic plasticity through Arc, phosphorylated cAMP response element-binding protein (pCREB), N -methyl-d-aspartate receptor 1 (GluN1), and GluN2A/2B immunohistochemistry, which was performed on COX-2 knockout (KO) and wild-type (WT) mice. Extremely weak COX-2 immunoreactivity was detected in the hippocampal CA1-3 areas in addition to the dentate gyrus at P1. Conversely, COX-2 immunoreactivity was observed in the stratum pyramidale of the CA1-3 regions and in the outer granule cell layer of the dentate gyrus at P7. Additionally, although peak COX-2 immunoreactivity was observed in all hippocampal sub-regions, including the dentate gyrus at P14, it was significantly decreased at P14. Finally, COX-2 immunoreactivity and the distribution pattern seen at P56 in the hippocampal CA1-3 regions were similar to those observed at P28, whereas, they were identified in the inner half of the granule cell layer of the dentate gyrus. The western blot analysis revealed that the COX-2 protein levels peaked at P14 and were decreased at P28 and P56. Additionally, the number of Arc and pCREB immunoreactive cells as well as GluN1 and GluN2A/2B immunoreactivity of COX-2 KO mice were significantly decreased in the dentate gyrus when compared with that in WT mice. Taken together, the results of the present study suggest that COX-2 serves an important role in synaptic plasticity in the dentate gyrus and changes in the levels of its constitutive expression are associated with the hippocampal dentate gyrus postnatal development.

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Time Course of Postnatal Distribution of Doublecortin Immunoreactive Developing/Maturing Neurons in the Somatosensory Cortex and Hippocampal CA1 Region of C57BL/6 Mice

Cited by 9 publications

References 38 publications

Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice

Growth/differentiation factor 15 promotes EGFR signalling, and regulates proliferation and migration in the hippocampus of neonatal and young adult mice

Melatonin improves d‐galactose‐induced aging effects on behavior, neurogenesis, and lipid peroxidation in the mouse dentate gyrus via increasing pCREB expression

Postnatal changes in constitutive cyclooxygenase‑2 expression in the mice hippocampus and its function in synaptic plasticity

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