Time course of Newcastle disease virus-induced apoptotic pathways

Ravindra, P. V.; Tiwari, Ashok Kumar; Ratta, Barkha; Bais, Manish V.; Chaturvedi, Uttara; Palia, Sudesh Kumar; Sharma, Bhaskar; Chauhan, R.S.

doi:10.1016/j.virusres.2009.05.012

Cited by 48 publications

(41 citation statements)

References 24 publications

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“…4A). We propose that the late apoptosis activation may be due to a combination of overwhelming virus infection during the later cycles, generating cellular stress and activating receptor-mediated apoptosis pathways (8,31), the RIG-I-activated apoptosis pathway (2), and the proapoptotic activity of IFN (3). In support of the latter, NDV replication in A549-Bcl-xL cells induced a strong type I IFN response (Fig.…”

Section: Vol 85 2011 Selectivity Of Ndv For Apoptosis-resistant Celmentioning

confidence: 80%

“…Like many viruses, NDV induces apoptosis by activating the mitochondrial pathway (8,31). This causes opening of mitochondrial permeability transition pores and loss of mitochondrial membrane potential, leading to release of SMAC/DIABLO and cytochrome c. SMAC/DIABLO causes suppression of inhibitory apoptosis proteins (IAP), while cytochrome c binds to apoptotic protease activator factor 1 (APAF-1) and procaspase 9, forming the apoptosome which activates apoptosis (9).…”

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confidence: 99%

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Oncolytic Specificity of Newcastle Disease Virus Is Mediated by Selectivity for Apoptosis-Resistant Cells

Mansour

Palese

Zamarin

2011

J Virol

126

111

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Newcastle disease virus (NDV) is a negative-sense RNA virus that has been shown to possess oncolytic activity. NDV's selective replication in tumor cells has been previously suggested to be due to the lack of a proper antiviral response in these cells. Here we demonstrate that NDV possesses oncolytic activity in tumor cells capable of a robust type I interferon (IFN) response, suggesting that another mechanism underlies NDV's tumor specificity. We show that the oncolytic selectivity of NDV for tumor cells is dependent upon tumor cell resistance to apoptosis. Utilizing the human non-small-cell lung cancer cell line A549 overexpressing the antiapoptotic protein Bcl-xL, we show significant enhancement of oncolytic activity and NDV replication. Interestingly, while the Bcl-xL-overexpressing cells were resistant to apoptotic stimuli induced by chemotherapeutic agents and early viral replication, during the subsequent viral cycles, we observed a paradoxical increase in apoptosis in response to NDV. The increased oncolytic activity seen was secondary to enhanced viral replication and syncytium formation. The induction of a type I IFN response was enhanced in Bcl-xL cells. Overall, these findings propose a new mechanism for cancer cell specificity for NDV, making it an attractive anticancer agent for chemoresistant tumors with enhanced antiapoptotic activity.

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Section: Vol 85 2011 Selectivity Of Ndv For Apoptosis-resistant Celmentioning

confidence: 80%

mentioning

confidence: 99%

Oncolytic Specificity of Newcastle Disease Virus Is Mediated by Selectivity for Apoptosis-Resistant Cells

Mansour

Palese

Zamarin

2011

J Virol

126

111

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“…Throughout the study, the mean TLC in Group I was significantly (P < 0.05) higher than the mean TLC in Group IV. The leucocytosis may be attributed to increased production of leukocytes in the haematopoietic tissues (Yongola et al, 2006;Ravindraa et al, 2009). The primary consequence of low leukocyte count in stressed chickens is suppression of the immune system and increased susceptibility to disease (Wambura, 2009).…”

Section: Discussionmentioning

confidence: 99%

Immunologic effects of Moringa oleifera methanolic leaf extract in chickens infected with Newcastle disease virus (kudu 113) strain

Eze

Okwor²,

Okoye³

et al. 2013

Afr. J. Pharm. Pharmacol.

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This study was aimed at evaluating the immune boosting potential of crude methanolic extract of Moringa oleifera in chickens experimentally challenged with Newcatle disease (ND) virus. One hundred and twenty forty two (42) day old chicks were randomly divided into four equal groups. Groups I and II were given daily oral treatment of methanolic extract of M. oleifera at 200 mg/kg body weight until day 56 of age. Groups II and III were vaccinated with the La Sota strain of ND vaccine. Group I was not vaccinated while group IV was left as untreated/unvaccinated control. All the groups were challenged with the velogenic strain of ND virus (VNDV) on day 56. Following challenge, the birds were assessed for cellular and humoral immune responses. Data on cellular and humoral immune responses were analysed using the statistical package for social sciences (SPSS). Increases in total and differential cell numbers and haemagglutination inhibition (HI) titre in the extract-treated groups did not correlate with total protection against ND. M. oleifera extract increased ND HI titre and the total and differential leukocyte counts in the treated and unvaccinated group I birds much more than those of treated and vaccinated group II birds, hence it could be recommended as a prophylactic treatment against ND in non vaccinated birds.

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“…The combined replication of Reo and NDV may enhance apoptosis. Indeed, infection with reovirus alone provokes apoptosis via the extrinsic pathway through the activation of the TRAILreceptor [42,43], while NDV-infected cells seem to die through an intrinsic apoptotic pathway [44,45]. Synergism between intrinsic and extrinsic apoptosis has been reported before [46].…”

Section: Discussionmentioning

confidence: 83%

The combined effects of oncolytic reovirus plus Newcastle disease virus and reovirus plus parvovirus on U87 and U373 cells in vitro and in vivo

et al. 2011

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Previous results had documented oncolytic capacity of reovirus, parvovirus and Newcastle disease virus (NDV) on several tumor cell types. To test whether combinations of these viruses may increase this capacity, human U87- and U373-glioblastoma cells, in vitro or xenografted into immuno-compromised mice, were subjected to simultaneous double infections and analyzed. Our results show that reovirus (serotype-3) plus NDV (Hitcher-B1) and reovirus plus parvovirus-H1 lead to a significant increase in tumor cell killing in vitro in both cell lines (Kruskal-Wallis test, P < 0.01) and in vivo. Immunofluorescence and flow cytometry analyses demonstrated the simultaneous replication of the viruses in nearly all cells (>95%) after combined infection. These data thus indicate that a synergistic anti-tumor effect can be achieved by the combined infection with oncolytic viruses.

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Time course of Newcastle disease virus-induced apoptotic pathways

Cited by 48 publications

References 24 publications

Oncolytic Specificity of Newcastle Disease Virus Is Mediated by Selectivity for Apoptosis-Resistant Cells

Oncolytic Specificity of Newcastle Disease Virus Is Mediated by Selectivity for Apoptosis-Resistant Cells

Immunologic effects of Moringa oleifera methanolic leaf extract in chickens infected with Newcastle disease virus (kudu 113) strain

The combined effects of oncolytic reovirus plus Newcastle disease virus and reovirus plus parvovirus on U87 and U373 cells in vitro and in vivo

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