Time course of lead induced proteomic changes in gill of the Antarctic limpet Nacella Concinna (Gastropoda: Patellidae)

Piechnik, Cláudio Adriano; Höckner, Martina; Souza, Maria Rosa Dmengeon Pedreiro de; Donatti, Lucélia; Tomanek, Lars

doi:10.1016/j.jprot.2016.04.036

Cited by 8 publications

(3 citation statements)

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“…To determine the protein alterations in response to heavy metal stress in polar yeast, a proteomic analysis by the method of 2‐DE and MALDI‐TOF/TOF MS was performed. To the best of our knowledge, proteomic analysis from the Antarctic organisms aimed at archaeon (Liao et al., ), bacteria (Kulkarni, Swamy, & Jagannadham, ), alga (Park, Jin, & Lee, ), and animals (Piechnik, Höckner, de Souza, Donatti, & Tomanek, ), and no study of proteome responses induced by heavy metal in Antarctic yeast was reported. Here, results of proteomics researches indicated that 51 DEPs were identified in Antarctic yeast R. mucilaginosa AN5, and they were classified into 6 categories by functional analysis, including carbohydrate and energy metabolism, nucleotide and amino acid metabolism, protein folding, antioxidant system, signaling, and unknown function proteins.…”

Section: Discussionmentioning

confidence: 99%

“…To determine the protein alterations in response to heavy metal stress in polar yeast, a proteomic analysis by the method of 2-DE and MALDI-TOF/TOF MS was performed. To the best of our knowledge, proteomic analysis from the Antarctic organisms aimed at archaeon (Liao et al, 2016), bacteria (Kulkarni, Swamy, & Jagannadham, 2015), alga (Park, Jin, & Lee, 2008), and animals (Piechnik, Höckner, de Souza, Donatti, & Tomanek, 2017), and no study of proteome responses induced by heavy metal in Antarctic yeast was reported.…”

Section: Ta B L E 1 (Continued)mentioning

confidence: 99%

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Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic

et al. 2018

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Heavy metal pollution in Antarctic is serious by anthropogenic emissions and atmospheric transport. To dissect the heavy metal adaptation mechanisms of sea-ice organisms, a basidiomycetous yeast strain AN5 was isolated and its cellular changes were analyzed. Morphological, physiological, and biochemical characterization indicated that this yeast strain belonged to Rhodotorula mucilaginosa AN5. Heavy metal resistance pattern of Cd > Pb = Mn > Cu > Cr > Hg was observed. Scanning electron microscopic (SEM) results exhibited altered cell surface morphology under the influence of copper metal compared to that with control. The determination of physiological and biochemical changes manifested that progressive copper treatment significantly increased antioxidative reagents content and enzymes activity in the red yeast, which quench the active oxygen species to maintain the intercellular balance of redox state and ensure the cellular fission and growth. Comparative proteomic analysis revealed that, under 2 mM copper stress, 95 protein spots were tested reproducible changes of at least 10-fold in cells. Among 95 protein spots, 43 were elevated and 52 were decreased synthesis. After MALDI TOF MS/MS analysis, 51 differentially expressed proteins were identified successfully and classified into six functional groups, including carbohydrate and energy metabolism, nucleotide and protein metabolism, protein folding, antioxidant system, signaling, and unknown function proteins. Function analysis indicated that carbohydrate and energy metabolism-, nucleotide and protein metabolism-, and protein folding-related proteins played central role to the heavy metal resistance of Antarctic yeast. Generally, the results revealed that the yeast has a great capability to cope with heavy metal stress and activate the physiological and protein mechanisms, which allow more efficient recovery after copper stress. Our studies increase understanding of the molecular resistance mechanism of polar yeast to heavy metal, which will be benefitted for the sea-ice isolates to be a potential candidate for bioremediation of metal-contaminated environments.

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Section: Discussionmentioning

confidence: 99%

Section: Ta B L E 1 (Continued)mentioning

confidence: 99%

Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic

et al. 2018

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“…There are several environmental proteomics reports indicating low amounts of proteins, possibly related to the biological complexity of the organisms or the proteome range methodologies available 83 , resulting in low correlations between RNA and protein levels. Many ecotoxicological reports refer to around 100 or less proteins identified in chitin based invertebrate species 86 – 89 but also in other invertebrates 90 , 91 . Despite the low correlation rates observed here, the information provided by these two levels of datasets revealed to be complementary and corroborative.…”

Section: Discussionmentioning

confidence: 99%

An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide

Simões

Novais

Natal-da-Luz

et al. 2018

Sci Rep

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The use of integrative molecular approaches can aid in a comprehensive understanding of the effects of toxicants at different levels of biological organization, also supporting risk assessment. The present study aims to unravel the toxicity mechanisms of a widely used herbicide to the arthropod Folsomia candida exposed in a natural soil, by linking effects on reproduction, proteomics and genome-wide gene expression. The EC50 effects on reproduction over 4 weeks was 4.63 mg glyphosate/kg of soil. The formulation included a polyethoxylated tallowamine as an adjuvant, which at 50% effect on reproduction had an estimated concentration of 0.87–1.49 mg/kg of soil. No effects were observed on survival and reproduction when using the isolated active substance, pointing the toxicity of the formulated product to the co-formulant instead of the active ingredient, glyphosate. RNA sequencing and shotgun proteomics were applied to assess differential transcript and protein expressions between exposed and control organisms in time, respectively. Specific functional categories at protein and transcriptome levels were concordant with each other, despite overall limited correlations between datasets. The exposure to this formulation affected normal cellular respiration and lipid metabolism, inducing oxidative stress and leading to impairment in biological life cycle mechanisms such as molting and reproduction.

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Influence of Delipidation on Hg Analyses in Biological Tissues: A Case Study for an Antarctic Ecosystem

Cipro

Bustamante

Montone

2017

Water Air Soil Pollut

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The use of low-polarity organic solvents is widespread in cleanup/extraction processes in order to carry compounds of interest, remove interferences and separate phases, among other uses. A large number of studies have used delipidation to remove excess of lipids to analyse carbon stable isotopes in biological tissues for trophic and behavioural ecology investigations. In this context, the primary aim of this study is to assess the influence of one delipidation process on the results of total mercury (Hg) analyses and the possible use of delipidated samples from previous analyses, such as for stable isotopes, in Hg level determination. Samples of vegetation (angiosperm, lichens and mosses), invertebrates (krill and limpets), fish (marbled and black rockcod), bird liver and eggs (Antarctic, Gentoo and Adélie penguins, kelp gull, Antarctic tern, cape petrel and giant southern petrel) and pinniped tissues (Weddell seal, crabeater seal, southern elephant seal and Antarctic fur seal) were analysed for Hg before and after delipidation by cyclohexane. The difference between the two measurements ranged individually from −63 to 136% (in the moss Sanionia uncinata) and the averages ranged from −60 to 66% (in pinniped tissues). The proportion of organic Hg, which presents considerable lipophilicity, but also high affinity for sulfhydryl groups in proteins, might be responsible for such variability. Given the limitations of our study, we think it is safe to say that delipidated samples could not be used to infer total Hg values in non-delipidated ones.

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Time course of lead induced proteomic changes in gill of the Antarctic limpet Nacella Concinna (Gastropoda: Patellidae)

Cited by 8 publications

References 105 publications

Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic

Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic

An integrative omics approach to unravel toxicity mechanisms of environmental chemicals: effects of a formulated herbicide

Influence of Delipidation on Hg Analyses in Biological Tissues: A Case Study for an Antarctic Ecosystem

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