Time Course of Contrast Enhancement by Micro-CT with Dedicated Contrast Agents in Normal Mice and Mice with Hepatocellular Carcinoma

Rothe, Jessica; Rudolph, Ines; Rohwer, Nadine; Kupitz, Dennis; Gregor-Mamoudou, Betina; Derlin, Thorsten; Furth, Christian; Amthauer, Holger; Brenner, Winfried; Buchert, Ralph; Cramer, Thorsten; Apostolova, Ivayla

doi:10.1016/j.acra.2014.07.022

Cited by 30 publications

(28 citation statements)

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“…). In accord to our results, Rothe et al reported that ExiTron nano 12 000 yielded the strongest CE in the blood pool compared to eXIA 160 and ExiTron nano 6000 . The CAs eXIA 160, eXIA 160XL, Fenestra VC and LC showed a CE just shortly after injection.…”

Section: Discussionsupporting

confidence: 92%

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Comparison of small animal CT contrast agents

Mannheim

Kuebler

Quintanilla-Martı́nez

et al. 2016

Contrast Media & Molecular

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Non-invasive in vivo small animal computed tomography (CT) imaging provides high resolution bone scans but cannot differentiate between soft tissues. For most applications injections of contrast agents (CAs) are necessary. Aim of this study was to uncover the advantages and disadvantages of commercially available CT CAs (ExiTron nano 12 000 and 6000, eXIA 160 and 160XL, Fenestra VC and LC) regarding their pharmacokinetics, toxicological side-effects and the influence of anesthesia on the biodistribution, based on an injection volume of 100 μL/25 g body weight. The pharmacokinetics of the CAs were determined for up to five days. The CA-induced toxicological/physiological side-effects were evaluated by determining blood counts, liver enzymes, thyroxine and total protein values, pro-inflammatory mediators (messenger ribonucleic acid (mRNA)), histology and immunohistochemistry. ExiTron nano 12 000 and 6000 yielded a long-term contrast enhancement (CE) in the liver and spleen for up to five days. Some of the evaluated CAs did not show any CE at all. Anesthesia did not impair the CAs' biodistribution. The CAs differentially affected the body weight, blood counts, liver enzymes, thyroxine and total protein values. ExiTron nano 12 000 and 6000 induced histiocytes in the liver and spleen. Moreover, ExiTron nano 12 000 and eXIA 160 enhanced tumor necrosis factor (TNF) mRNA expression levels in the kidneys. Thus, we recommend ExiTron nano 12 000 and 6000 when multiple injections should be avoided. We recommend careful selection of the employed CA in order to achieve an acceptable CE in the organs of interest and to avoid influences on the animal physiology. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Discussionsupporting

confidence: 92%

“…The relative expression was normalized to the housekeeping gene Aldolase and the control group (n = 2). pool compared to eXIA 160 and ExiTron nano 6000 (30). The CAs eXIA 160, eXIA 160XL, Fenestra VC and LC showed a CE just shortly after injection.…”

Section: Discussionmentioning

confidence: 90%

Comparison of small animal CT contrast agents

Mannheim

Kuebler

Quintanilla-Martı́nez

et al. 2016

Contrast Media & Molecular

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“…The blood half‐life in C57BL/6 mice is ~4 hours with complete clearance from the blood in about 24 hours. Clearance is mainly a result of uptake by Kuppfer cells in the liver (supplier information). Previous applications have mostly been angiography and liver imaging …”

Section: Methodsmentioning

confidence: 99%

Bleed volume of experimental knee haemarthrosis correlates with the subsequent degree of haemophilic arthropathy

et al. 2019

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Background Haemophilic arthropathy is the main morbidity of haemophilia. The individual pathological response to the same number of clinically evident joint bleeds is highly variable; thus, it remains unknown if certain joint bleeding characteristics are critical for the development of arthropathy. Aim To study the relation between bleed volume and subsequent development of arthropathy, we aimed to develop quantitative in vivo imaging of active joint bleeds in a mouse model of haemophilia. Methods Haemophilia A (F8‐KO) and wild‐type (WT) mice were IV‐dosed with a micro‐CT blood pool contrast agent prior to an induced knee haemarthrosis or sham procedure. The mice were micro‐CT scanned five times the following 2 days to characterise and quantify the induced haemarthrosis in vivo. On Day 14, the mice were euthanized and pathological changes evaluated by histology and micro‐CT. Additionally, bleeding characteristics in vehicle‐treated F8‐KO mice were compared with those of recombinant FVIII (rFVIII)‐treated F8‐KO mice. Results F8‐KO mice had a significantly larger bleed volume than WT mice at all scan time points. The bleed volume 12 hours after induction of haemarthrosis correlated with the subsequent degree of arthropathy. Presence of µCT‐detectable bone pathology was associated with a significantly increased bleed volume among F8‐KO mice. rFVIII treatment significantly reduced bleed volume in F8‐KO mice. Conclusion Quantitative in vivo contrast‐enhanced micro‐CT imaging can be used to characterize and quantify joint bleeds in a mouse model of haemophilic arthropathy. The bleed volume correlates with the subsequent degree of arthropathy.

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“…Measurements of tumor vascular density in early-phase imaging and total contrast accumulation in late-phase imaging have also been used in two mouse models of lung cancer to differentiate different aggressiveness levels in tumors (Badea et al 2012). Iodine-containing nanoparticle contrast agents have also been used for tumor imaging in two other models of lung cancer (Kindlmann et al 2005, Anayama et al 2013) and a mouse model of liver cancer (Rothe et al 2015). Gold nanoparticles have also been used for passive tumor targeting in mouse models of breast and brain cancer (Hainfeld et al 2006(Hainfeld et al , 2013.…”

Section: Cancer Imagingmentioning

confidence: 99%

Small Animal X-ray Computed Tomography

Badea¹

2017

Handbook of X-Ray Imaging

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Time Course of Contrast Enhancement by Micro-CT with Dedicated Contrast Agents in Normal Mice and Mice with Hepatocellular Carcinoma

Cited by 30 publications

References 31 publications

Comparison of small animal CT contrast agents

Comparison of small animal CT contrast agents

Bleed volume of experimental knee haemarthrosis correlates with the subsequent degree of haemophilic arthropathy

Small Animal X-ray Computed Tomography

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