Time course of Ca2+ concentration triggering exocytosis in neuroendocrine cells.

Chow, Robert H.; Klingauf, Jürgen; Neher, Erwin

doi:10.1073/pnas.91.26.12765

Cited by 178 publications

(149 citation statements)

References 30 publications

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“…It was shown that depolarization lasting between 2 and 40 ms support an increase in C m in rat melanotrophs (48). Dense-core vesicles that undergo depolarization-induced exocytosis require around 5 M Ca 2ϩ (49). The time constant of increase of the rapid C m component of around 30-40 ms (Fig.…”

Section: Discussionmentioning

confidence: 99%

Rapid regulated dense-core vesicle exocytosis requires the CAPS protein

Rupnik

Kreft

Sikdar

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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Although many proteins essential for regulated neurotransmitter and peptide hormone secretion have been identified, little is understood about their precise roles at specific stages of the multistep pathway of exocytosis. To study the function of CAPS (Ca 2؉ -dependent activator protein for secretion), a protein required for Ca 2؉ -dependent exocytosis of dense-core vesicles, secretory responses in single rat melanotrophs were monitored by patch-clamp membrane capacitance measurements. Flash photolysis of caged Ca 2؉ elicited biphasic capacitance increases consisting of rapid and slow components with distinct Ca 2؉ dependencies. A threshold of Ϸ10 M Ca 2؉ was required to trigger the slow component, while the rapid capacitance increase was recorded already at a intracellular Ca 2؉ activity < 10 M. Both kinetic membrane capacitance components were abolished by botulinum neurotoxin B or E treatment, suggesting involvement of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor)-dependent vesicle fusion. The rapid but not the slow component was inhibited by CAPS antibody. These results were further clarified by immunocytochemical studies that revealed that CAPS was present on only a subset of dense-core vesicles. Overall, the results indicate that dense-core vesicle exocytosis in melanotrophs occurs by two parallel pathways. The faster pathway exhibits high sensitivity to Ca 2؉ and requires the presence of CAPS, which appears to act at a late stage in the secretory pathway.capacitance ͉ pituitary cells ͉ rat melanotrophs

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Section: Discussionmentioning

confidence: 99%

Rapid regulated dense-core vesicle exocytosis requires the CAPS protein

Rupnik

Kreft

Sikdar

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

“…Future indepth studies to address this question are warranted, perhaps, using a combination of patch-clamp, high-resolution Ca 2ϩ imaging and amperometry of vesicle-loaded oxidizable "false transmitters," similar to that described for other cells (Chow et al, 1994;Zhang and Zhou, 2002;Sakaba et al, 2005).…”

Section: Molecular Basis For the Inhibition By /Ea Of Capsaicin-triggmentioning

confidence: 99%

Activation of TRPV1 Mediates Calcitonin Gene-Related Peptide Release, Which Excites Trigeminal Sensory Neurons and Is Attenuated by a Retargeted Botulinum Toxin with Anti-Nociceptive Potential

Meng¹,

Ovsepian²,

Wang³

et al. 2009

J. Neurosci.

220

193

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“…Neural secretion is a superlinear function of Ca 2ϩ concentration (30,31), implicating a possible synergism between sparks and Ca 2ϩ entry in controlling exocytosis. To appraise this possibility, we measured depolarization-induced vesicle secretion in the absence and presence of a RyR antagonist (whole-cell dialysis of 50 M ryanodine).…”

Section: Drg Sparks Directly Trigger Exocytosismentioning

confidence: 99%

Ca ²⁺ sparks and secretion in dorsal root ganglion neurons

Ouyang

Zheng

Qin

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Ca 2؉ sparks as the elementary intracellular Ca 2؉ release events are instrumental to local control of Ca 2؉ signaling in many types of cells. Here, we visualized neural Ca 2؉ sparks in dorsal root ganglion (DRG) sensory neurons and investigated possible role of DRG sparks in the regulation of secretion from the somata of the cell. DRG sparks arose mainly from type 3 ryanodine receptor Ca 2؉ release channels on subsurface cisternae of the endoplasmic reticulum, rendering a striking subsurface localization. Caffeine-or 3,7-dimethyl-1-(2-propynyl)xanthine-induced store Ca 2؉ release, in the form of Ca 2؉ sparks, triggered exocytosis, independently of membrane depolarization and external Ca 2؉ . The spark-secretion coupling probability was estimated to be between 1 vesicle per 6.6 sparks and 1 vesicle per 11

show abstract

Time course of Ca2+ concentration triggering exocytosis in neuroendocrine cells.

Cited by 178 publications

References 30 publications

Rapid regulated dense-core vesicle exocytosis requires the CAPS protein

Rapid regulated dense-core vesicle exocytosis requires the CAPS protein

Activation of TRPV1 Mediates Calcitonin Gene-Related Peptide Release, Which Excites Trigeminal Sensory Neurons and Is Attenuated by a Retargeted Botulinum Toxin with Anti-Nociceptive Potential

Ca ²⁺ sparks and secretion in dorsal root ganglion neurons

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Time course of Ca2+ concentration triggering exocytosis in neuroendocrine cells.

Cited by 178 publications

References 30 publications

Rapid regulated dense-core vesicle exocytosis requires the CAPS protein

Rapid regulated dense-core vesicle exocytosis requires the CAPS protein

Activation of TRPV1 Mediates Calcitonin Gene-Related Peptide Release, Which Excites Trigeminal Sensory Neurons and Is Attenuated by a Retargeted Botulinum Toxin with Anti-Nociceptive Potential

Ca 2+ sparks and secretion in dorsal root ganglion neurons

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Ca ²⁺ sparks and secretion in dorsal root ganglion neurons