Time-course gene expression profiling data of Triticum aestivum treated by supercritical CO2 garlic extract encapsulated in nanoscale liposomes

Kutasy, Barbara; Decsi, Kincső; Kiniczky, Márta; Hegedűs, Géza; Virág, Eszter

doi:10.1016/j.dib.2022.108287

Cited by 4 publications

(10 citation statements)

References 11 publications

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“…The mechanism of action was investigated by using genome-wide transcriptional profiling of genes associated with the defense response triggered by the ‘Garlic-lipo’. The results presented here are based on the QuantSeq 3′ mRNA sequencing experiments, which data have been previously reported by Kutasy et al (2022) [ 39 ].…”

Section: Introductionmentioning

confidence: 74%

“…The Illumina Gene Expression Profiling (GEx) library preparation, RNA sequencing, sequence read pre-processing, gene-level quantification, and differentially expressed gene (DEG) analysis were performed as described in our previous papers [ 39 , 41 ]. The RNA libraries were sequenced with a final output single-end, with 14–26 M x 85 bp long reads.…”

Section: Methodsmentioning

confidence: 99%

“…This transcript dataset was deposited in the Transcriptome Shotgun Assembly (TSA) database at DBJ/EMBL/GenBank under the accession GJUY00000000.1. To estimate transcript abundances, CountTable was created [ 39 ], which is accessible in Mendeley Data ( , accessed on 17 March 2022). DEGs were calculated based on the CountTable by applying a pairwise differential expression analysis as described by Hegedűs et al (2022) [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

“…A time-course expression analysis of the count data arising from the RNA-seq was performed to detect genomic features with significant temporal expression changes and with significant differences between the experimental groups [ 39 ]. The genes with significantly different expression levels were divided into nine clusters.…”

Section: Methodsmentioning

confidence: 99%

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‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (Triticum aestivum)

Kutasy

Kiniczky²,

Decsi

et al. 2023

Plants

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Recently, environmentally friendly crop improvements using next-generation plant biostimulants (PBs) come to the forefront in agriculture, regardless of whether they are used by scientists, farmers, or industries. Various organic and inorganic solutions have been investigated by researchers and producers, focusing on tolerance to abiotic and biotic stresses, crop quality, or nutritional deficiency. Garlic has been considered a universal remedy ever since antiquity. A supercritical carbon dioxide garlic extract encapsulated in nanoscale liposomes composed of plant-derived lipids was examined as a possible PB agent. The present study focused on the characterization of the genes associated with the pathways involved in defense response triggered by the liposome nanoparticles that were loaded with supercritical garlic extracts. This material was applied to Triticum aestivum in greenhouse experiments using foliar spraying. The effects were examined in a large-scale genome-wide transcriptional profiling experiment by collecting the samples four times (0 min, used as a control, and 15 min, 24 h, and 48 h after spraying). Based on a time-course expression analysis, the dynamics of the cellular response were determined by examining differentially expressed genes and applying a cluster analysis. The results suggested an enhanced expression of abscisic acid (ABA) pathway and pathogenesis-related (PR) genes, of which positive regulation was found for the AP2-, C2H2-, HD-ZIP-, and MYB-related transcription factor families.

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Section: Introductionmentioning

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (Triticum aestivum)

Kutasy

Kiniczky²,

Decsi

et al. 2023

Plants

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“…The development of products with biologically active agents focuses on improving yield and abiotic stress tolerance therefore, in this study, the effects of the newly developed liposomal formulation of SC-CO 2 botanical extracts (ELICE16INDURES) were examined. This formulation technology was successfully applied in our previous research, suggesting the easier uptake of the active ingredients [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Liposomal Formulation of Botanical Extracts may Enhance Yield Triggering PR Genes and Phenylpropanoid Pathway in Barley (Hordeum vulgare)

Hegedűs

Kutasy

Kiniczky³

et al. 2022

Plants

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This work aimed to study the plant conditioning effect and mode of action of a plant-based biostimulant used in organic farming. This new generation plant biostimulant, named ELICE16INDURES®, is rich in plant bio-active ingredients containing eleven supercritical botanical extracts encapsulated in nano-scale liposomes. The dose–response (10 to 240 g ha−1) relationship was tested in a field population of autumn barley (Hordeum vulgare) test crop, and underlying molecular mechanisms were studied. Applying nanotechnology, cell-identical nanoparticles may help the better uptake and delivery of active ingredients increasing resilience, vitality, and crop yield. The amount of harvested crops showed a significant increase of 27.5% and 39.9% interconnected to higher normalized difference vegetation index (NDVI) of 20% and 25% after the treatment of low and high dosages (20 and 240 g ha−1), respectively. Illumina NextSeq 550 sequencing, gene expression profiling, and KEGG-pathway analysis of outstanding dosages indicated the upregulation of pathogenesis-related (PR) and other genes—associated with induced resistance—which showed dose dependency as well.

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Liposomes for delivery of substances for other (non-therapeutic) applications

Logigan,

Peptu,

Peptu

2024

Liposomes in Drug Delivery

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Time-course gene expression profiling data of Triticum aestivum treated by supercritical CO2 garlic extract encapsulated in nanoscale liposomes

Cited by 4 publications

References 11 publications

‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (Triticum aestivum)

‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (Triticum aestivum)

Liposomal Formulation of Botanical Extracts may Enhance Yield Triggering PR Genes and Phenylpropanoid Pathway in Barley (Hordeum vulgare)

Liposomes for delivery of substances for other (non-therapeutic) applications

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