Time course gene expression data in colon of mice after exposure to food-grade E171

Abstract

We investigated gene expression responses in BALB/c mice exposed by gavage to 5 mg/kg bw/day of E171 for 2, 7, 14 and 21 days. Food additive E171 (titanium dioxide) has been shown to induce oxidative stress and DNA damage in vitro as well as facilitating growth of colorectal tumours in vivo. Full genome expression changes of the colon of mice were investigated by using Agilent SurePrint G3 mouse Gene exp 60kv2 microarrays slides. The data presented in this DiB include all differentially expressed for each time point with EntrezGeneID, gene symbols, gene names and Log2FC as well as genes included in pathways after over-representation analysis in ConsensusPathDataBase. The functions of these genes in relation to the colon were described in our associated article (Proquin et al., 2017 in press) [1]. Raw and normalized gene expression data are available through NCBI GEO (GEO accession: GSE92563).

EFSA statement on the review of the risks related to the exposure to the food additive titanium dioxide (E 171) performed by the French Agency for Food, Environmental and Occupational Health and Safety (ANSES)

“…Although with several limitations, ANSES considered that some of the effects seem consistent with previous observations (Urrutia‐Ortega et al., ; Bettini et al., ) of a potential tumour‐promoting potential of titanium dioxide and, like those two studies, the study by Proquin et al. (,b,c) would deserve further investigation in specifically designed and adequately conducted studies.…”

“…With respect to the new findings identified in the current review by ANSES, there are reportedly gene expression changes in an initiation/inflammation model in mice as well as epigenetic effects suggested primarily by histone expression changes that may ultimately facilitate tumour induction and promotion (Proquin et al., ,b,c). Although with several limitations, ANSES considered that some of the effects seem consistent with previous observations (Urrutia‐Ortega et al., ; Bettini et al., ) of a potential tumour‐promoting potential of titanium dioxide and, like those two studies, the study by Proquin et al.…”

“…On the basis of the effects reported in the Proquin et al. (,b,c) and, previously, in other studies (Urrutia‐Ortega et al., ; Bettini et al., ), ANSES recommended to further investigate the tumour‐promoting potential of E 171 in the colon. This concern is expected to be addressed by the above mentioned 100‐day study commissioned by industry.…”

Gene expression profiling in colon of mice exposed to food additive titanium dioxide (E171)

“…Furthermore in the same family as GPCRs, DEG were regulated in molecular pathways such as olfactory signalling, olfactory transduction and odorant GPCRs. More details about the fold change and the direction of expression of the genes are presented in Table 5, in ref (Proquin et al, 2017b).…”

Nanoparticle Effects on Stress Response Pathways and Nanoparticle–Protein Interactions

“…Regarding TiO 2 NPs, treatment of male CD-1 mice with anatase TiO 2 NPs at 64 and 320 mg/kg body-weight/day for 14 weeks was detrimental, raised the blood glucose level without changing the blood insulin level, induced oxidative stress and inflammation, and showed disruption of the insulin signaling pathway with increased IRS-1 phosphorylation and decreased phosphorylation of Akt [ 289 ]. Additionally, ingestion of the TiO 2 food additive, T171, at 5 mg/kg body-weight/day by BALB/c mice resulted in altered gene expression in the mouse colon cells for several insulin related genes [ 290 ]. Microarray analysis of human colon carcinoma Caco-2 cells treated with T171 altered gene expression in the insulin processing pathway [ 42 ].…”