Time‐course expression of polypeptides carrying blood group antigens during human erythroid differentiation

Abstract

The time course expression of blood group antigens was examined by flow cytometry using a two-phase liquid culture system that supports the proliferation and maturation of human erythroid progenitors from adult peripheral blood. The progression towards erythroid differentiation was followed by the expression changes of the transferrin receptor (CD71++) and glycophorin A (GPA+). Four main categories of blood group markers were identified: (i) those characterized by an early expression like ABO (A), Kell (K:2) and Rh50 which were detected in the Epo-independent phase 1, (ii) those including GPC (Gerbich, Ge antigens) and Fy6 which were expressed in the late phase 1, (iii) GPA (MN antigens), Wrb (Band 3/GPA interaction), Rh(D, Cc/Ee) and LW which appeared during the Epo-dependent phase 2 and (iv) those like Jk3 and Lub which were expressed in late phase 2. Regarding blood group molecules exhibiting adhesive properties (LW/ICAM-4, Oka and Lu) the most significant event was a sharp decrease of Oka (neurothelin) expression with the concomitant loss of ICAMs expression during the later stage of differentiation. These studies suggest that Oka, ICAMs and LW might contribute to the adhesive interactions involved in the formation of erythroblastic islands and attachment to stroma cells and the extracellular matrix. We also noted an asynchronous expression of the proteins that compose the core of the Rh complex, since Rh50 glycoprotein was expressed earlier than Rh(D, CE) proteins.

Modulation of erythroid adhesion receptor expression by hydroxyurea in children with sickle cell disease

“…Mononuclear cells from peripheral blood were isolated and cultured using a two-phase liquid culture system as described before 27 with some modifications during the erythropoietin-dependent phase. Indeed, erythroid progenitors were cultured in normal oxygen conditions (21% O2) during this phase to avoid sickling of precursors, 28 although it is known that cell growth is favored in hypoxic conditions (5% O2).…”

Investigating the key membrane protein changes during in vitro erythropoiesis of protein 4.2 (-) cells (mutations Chartres 1 and 2)

“…42 It contradicts an earlier report showing late expression of protein 4.2 during erythropoiesis (orthochromatic stage), with band 3 expressed earlier at the basophilic stage. 40,41 However, an earlier simultaneous expression profile is in keeping with the known dependence of protein 4.2 on band 3 expression. 8,[10][11][12] The level and association of band 3 and protein 4.2 remains constant after 72h (normoblast stage) suggesting that the majority of protein 4.2 may already be associated with band 3 early in differentiation.…”

Red blood cell-incompatible allogeneic hematopoietic progenitor cell transplantation

“…In vitro studies, for example, demonstrated the expression of ABO and Kell antigens during the EPO-independent phase of colony growth, with other antigens such as Rh developing during the later EPO-dependent phase and Jk and Lu antigens appearing last. 79,80 The primary consequence of delayed red cell recovery is the need for continued red cell transfusions and the development of transfusional hemachromatosis. The risk appears to be higher in recipients of older age, a donor of blood group A, and use of certain (but not all) non-myeloablative conditioning regimens.…”