Time and temperature dependent changes of ADP- and collagen-induced and “spontaneous” aggregation

Breddin, K; Ziemen, M.; Bauer, O.; Herrmann, Wolfgang; Schaudinn, L.; Schlösser, U. G.; Winterhagen, A.; Krzywanek, H. J.

doi:10.1016/0049-3848(80)90034-1

Cited by 32 publications

(12 citation statements)

References 22 publications

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“…To prepare platelet rich plasma (PRP), citrate anticoagulated blood was centrifuged at 120g for 15 minutes (Megafuge, Heraeus, Thermo) [12]. The supernatant (PRP) was carefully removed and transferred to plastic test tubes.…”

Section: Platelet Rich Plasmamentioning

confidence: 99%

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Interaction of thrombocytes with poly(ether imide): The influence of processing

Braune¹,

Lange²,

Richau³

et al. 2010

Clinical Hemorheology and Microcirculation

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The processing of polymers for blood contacting devices can have a major influence on surface properties. In this study, we fabricated poly(ether imide) (PEI) membranes and films to investigate the effects of the processing on physicochemical surface properties by atomic force microscopy (AFM), scanning electron microscopy, contact angle as well as zeta potential measurements. A static platelet adhesion test was performed to analyze the thrombogenicity of both devices. While contact angle measurements showed similar levels of hydrophobicity and zeta potential values were equivalent, mean surface roughness as well as surface energies in the dispersive part were found to be increased for the PEI membrane. The static platelet adhesion test showed a significantly decreased number of adherent platelets per surface area on the PEI film (178.98 ± 102.70/45000 m 2 ) compared to the PEI membrane (504 ± 314.27/45000 m 2 ) and, consequently, revealed evidence for higher thrombogenicity of the PEI membrane. This study shows that processing can have a significant effect on platelet adhesion to biomaterials, even though, molar weight was identical. Thrombogenicity of polymer-based cardiovascular devices, therefore, have to be evaluated at the final product level, following the entire processing procedure.A wide range of polymers like polyurethanes, silicones or PEG-based copolymers have received growing attention for biomedical applications due to their better hemocompatibility and processing features compared to metals [26], whereupon the ideal hemocompatible surface inhibits any platelet adhesion [20]. Several parameters seem to influence hemocompatibility. Chapman et al. concluded that hydrophilic, noncharged surfaces, without hydrogen-donors are more resistant to protein adsorption and are able to prevent platelet adherence [13]. In addition the processing itself can lead to changes e.g. in surface roughness which can influence platelet adhesion as well [16,17,31,33,42].In the present study the influence of different processing of poly(ether imide) (PEI, Ultem 1000 ® , General Electric, USA) on the interaction of blood platelets with poly(ether imide) (PEI) was analyzed. PEI is non-toxic, sterilizable [1], processable to different formed-bodies [4,5], and enables a wide range of surface modifications by straightforward wet chemistry [2, 3] and was already evaluated as a biomaterial [6,24,32,35,36] suitable for applications in regenerative medicine [30,41].Since platelet interactions are controlled by physical and chemical characteristics [26], including their possible distribution in submicroscopical domains and surface roughness [37], characteristic surface parameters were analyzed. To reveal the influences of processing on surface properties, membranes and films from PEI were fabricated and the effects on the physicochemical properties and thrombogenicity investigated. Material and methods Polymer preparationAll processed devices were fabricated from the same charge of the polymer. PEI (Ultem 1000, General Electric, USA)...

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Section: Platelet Rich Plasmamentioning

confidence: 99%

“…An elementary screening method to estimate the hemocompatibility of cardiovascular implant materials is the thrombocyte spreading test [11,12]. This static test evaluates the adhesion, activation, spreading and aggregation of thrombocytes on the surface of materials (thrombosis testing, see ISO 10993-4).…”

Section: Introductionmentioning

confidence: 99%

Interaction of thrombocytes with poly(ether imide): The influence of processing

Braune¹,

Lange²,

Richau³

et al. 2010

Clinical Hemorheology and Microcirculation

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“…A performance of the labeling procedure at room tempera ture was preferred to a procedure at 37 °C, since the capacity of platelets to react to ADP-induced aggregation at room tempera ture remains almost unaffected for several hours [3]. Furthermore the ATP level essen tial for the maintenance of the platelet me tabolism shows the same negligible degree of reduction at room temperature as well as at 37 °C [4,20], Finally the survival time of platelets stored at room temperature is nor mal [17], the morphological changes are small [6] and reversible [22], A partial activation of blood platelets dur ing the different steps of the labeling proce dure cannot completely be excluded, but any possible activation can be counteracted by using acid citrate as anticoagulant and by adjusting the platelet-rich plasma or the platelet suspensions, respectively, to pH 6.5 during labeling [13].…”

Section: Discussionmentioning

confidence: 99%

Maintenance of Platelet Viability after Platelet-Labeling with Fluorescein Isothiocyanate

Klaverkamp¹,

Völkl²

1984

Pathophysiol Haemos Thromb

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Possible effects of an ex vivo fluorescent labeling procedure of blood platelets on the platelet viability were examined by different function tests. The degree of the impairment of the platelet function proved to be principally correlated with the dye concentration used during the labeling procedure. However, the physiological properties of the labeled platelets obviously remain unrestricted by using intraplatelet fluorescein isothiocyanate (FITC) concentrations below 0.55 mg/10¹¹ platelets, corresponding to 8.5 × 10⁶ FITC molecules bound to one platelet. A procedure for fluorescent labeling of blood platelets is described which is considered to be preferably suitable to prepare platelets for vital microscopic platelet observations.

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“…Duke's bleeding time, ADP and collagen-induced platelet aggregation [8], plate let spreading [9], platelet ADP and ATP content [10], platelet shape change [11], factor-VIII-related antigen [VIIIR-Ag) [12], plasma ristocetin-cofactor (VIIIR:RC) [13], factor-VIII-coagulant activity (VIII-Q [14], platelet adhesivenes [15]. Platelet volume dis tribution was measured using a Clay Adams Ultraflo 100 with a Nuclear Data Channelizer and a Commo dore 8032 microcomputer with printer.…”

Section: Methodsmentioning

confidence: 99%

On the Retraction of Collagen and Fibrin Induced by Normal, Defective and Modified Platelets

Jeleńska¹,

Kopeć²,

Breddin³

1985

Pathophysiol Haemos Thromb

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Fibrin clot retraction (FCR) and collagen gel retraction (CGR) were studied in patients with inherited platelet defects, i.e. in Glanzmann’s thrombasthenia, Hermansky-Pudlak syndrome, May-Hegglin anomaly, giant platelet syndrome, as well as in patients with von Willebrand disease and factor XIII deficiency. FCR was abnormal only in thrombasthenia, while CGR was found to be reduced in 2 patients with Hermansky-Pudlak syndrome and in 4 out of 5 cases with von Willebrand disease. Both FCR and CGR were normal in May-Hegglin anomaly, giant platelet syndrome and severe factor XIII deficiency. In none of the examined bleeding disorders was a concomitant FCR and CGR reduction detected. Natural polyamines and anti-fibronectin antibodies did not affect platelet potency in FCR or CGR. Peroxidation of platelet membrane components by sodium periodate abolished the platelet-induced FCR and CGR. This effect was reversed by subsequent reduction by sodium borohydride.

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Time and temperature dependent changes of ADP- and collagen-induced and “spontaneous” aggregation

Cited by 32 publications

References 22 publications

Interaction of thrombocytes with poly(ether imide): The influence of processing

Interaction of thrombocytes with poly(ether imide): The influence of processing

Maintenance of Platelet Viability after Platelet-Labeling with Fluorescein Isothiocyanate

On the Retraction of Collagen and Fibrin Induced by Normal, Defective and Modified Platelets

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