Time and temperature before processing influence the recovery of umbilical cord blood hematopoietic progenitors

Tsagias, Nikolaos; Kouzi-Koliakos, Kokkona; Karagiannis, Vasileios; Alamdari, Daryoush Hamidi; Koliakos, George

doi:10.1111/j.1537-2995.2007.01351.x

Cited by 11 publications

(14 citation statements)

References 7 publications

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“…Published data are inconsistent and show either storage at ambient temperature for 24 h [21] or storage at 4 °C being superior [22]. On the contrary and consistent with our data, other publications reported no differences between refrigerated storage and storage at room temperature [23,24].…”

Section: Discussioncontrasting

confidence: 55%

Evaluation of Quality Parameters for Cord Blood Donations

Salge-Bartels

Huber²,

Kleiner³

et al. 2009

Transfus Med Hemother

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Background: Umbilical cord blood (CB) is widely used for hematopoietic stem cell transplantation and holds promise for the development of innovative medicinal products. In order to find out whether the conditions for collection and storage before processing might have an impact on the quality of CB preparations, viability and the clonogenic potential were assessed. Methods: CB was collected under field conditions. Flow cytometry was used to determine leukocytes, CD34/CD45+ cells, viability, and nucleated red blood cells (NRBC). Clonogenic activity was determined using isolated mononuclear cells (MNC). Results: Neither plasma citrate concentrations nor storage temperature (within 24 h) affected cell viability or colony formation. After storage for 49–80 h, leukocyte viability declined by about 16% compared to CB stored up to 24 h. In contrast, the clonogenic activity and CD34/CD45+ cell content were not affected. A higher gestational age was associated with a lower yield of clonogenic activity compared to midterm deliveries. NRBC varied widely (median 7.3%; range 0.63–17.3%) without relation to gestational age or colony formation. There was a close correlation between the percentage of viable CD34/CD45+ cells and colony formation (r = 0.77 for CFU-GM; r = 0.75 for CFU-C). Conclusions: The content of viable CD34/CD45+ cells represents the clonogenic activity of CB preparations. Therefore, determination of viable CD34/CD45+ cells should be generally performed as a routine quality control assay.

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Section: Discussioncontrasting

confidence: 55%

Evaluation of Quality Parameters for Cord Blood Donations

Salge-Bartels

Huber²,

Kleiner³

et al. 2009

Transfus Med Hemother

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“…The extension of the time from collection to processing was associated with a decrease of cell viability. These findings are in accord with the results from other studies [5,6,13,21,26,27]. Some authors [28] considered that temperature is a key parameter and extended …”

Section: Discussionsupporting

confidence: 95%

“…Thus, the storage temperature of UCB units from collection to cryopreservation seems to be an essential factor that influences the cell viability and contributes to maintaining the final product quality. The controversial results show that UCB units must be stored before processing at 4°C [4][5][6][7] or at room temperature [8][9][10]. Other authors consider that there are no differences reflected in the quality of transplant units depending on the storage temperature [11][12][13].…”

Section: Introductionmentioning

confidence: 89%

Factors which can influence the quality related to cell viability of the umbilical cord blood units

Dulugiac

Horeanga²,

Torcatoru³

et al. 2014

Transfusion and Apheresis Science

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“…It is unclear how the addition of heparin or ACD‐A contributes to lactic acid secretion or affects other aspects of cellular metabolism. Studies of storage media on the cell viability and recovery of short‐term storage of PBSCs have been reported, but none addressed the issue of lactic acid build‐up, cell viability, and stem cell function 12‐15 …”

Section: Discussionmentioning

confidence: 99%

“…Studies of storage media on the cell viability and recovery of short-term storage of PBSCs have been reported, but none addressed the issue of lactic acid build-up, cell viability, and stem cell function. [12][13][14][15] A number of previous studies have indicated that short-term storage of PBSCs at a refrigerated temperature does not affect TNC and CFU recovery nor trypan blue viability. [16][17][18][19][20] Many also found refrigerated temperatures to be optimal for recovery of colony-forming and long-term culture-initiating cells during short-term storage.…”

Section: Number Of Productsmentioning

confidence: 99%