Time and space resolved uptake study of silicananoparticles by human cells

Shapero, Kayle; Fenaroli, Federico; Lynch, Iseult; Cottell, David C.; Salvati, Anna; Dawson, Kenneth A.

doi:10.1039/c0mb00109k

Cited by 215 publications

(265 citation statements)

References 22 publications

(23 reference statements)

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“…9 Although we did not observe nanoparticle agglomeration (Table 2), at least in the first 6 hours, this was not the same in serum free conditions and, even if in all cases the uptake in serum free conditions was higher than in cMEM, the internalisation levels were very difficult to reproduce quantitatively in independent experiments.…”

Section: Resultsmentioning

confidence: 58%

“…We may conclude that uptake was an energy dependent process, however the extent of the decrease was not as strong as when performing the same experiment in cMEM. 9 Further EM and confocal imaging clearly explained this difference (also in Supplementary Figure S3). There, it was possible to see, that in serum free conditions, large clusters of nanoparticles were present on the cell membrane and this could affect the flow cytometry results.…”

Section: Resultsmentioning

confidence: 91%

“…9 This was particularly clear after long exposure time (24 h), where high level of colocalisation could be seen with lysosomal marker (LAMP1) positive structures, even though in these conditions not all of the nanoparticles were (yet) found there. This could also be related to the presence of nanoparticles which seemed free in the cytosol at EM analysis.…”

Section: Resultsmentioning

confidence: 93%

“…1,9,26,[40][41][42][43][44] Figure 1 shows a comparison of the kinetics of uptake of silica nanoparticles in A549 cells in serum free and complete media, obtained by flow cytometry, as described in the Methods. As anticipated in Supplementary Figure S1, the kinetic study also clearly indicated that uptake was always higher when the nanoparticles were exposed to cells in SF.…”

Section: Resultsmentioning

confidence: 99%

“…A series of details of typical nanoparticle locations inside the cells at larger magnification is given in The time resolved electron microscopy analysis showed that in serum free conditions, for the lower concentration and shorter incubation times silica nanoparticles were mainly found engulfed in vesicles (such as in cMEM) 9 along the endo-lysosomal pathway. Nanoparticles free in the cytosol were also observed (most of them closer to the cell membrane, as in the images in Figure 3), especially at longer exposure times or at higher nanoparticle concentration.…”

Section: Resultsmentioning

confidence: 99%

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Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells

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Section: Resultsmentioning

confidence: 58%

Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells

et al. 2012

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Silica, Amorphous

Gräf

2018

Kirk-Othmer Encyclopedia of Chemical Technology

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Amorphous or noncrystalline silica is silicon dioxide ( SiO 2 ) that does not have a crystalline structure. Amorphous silica can be naturally occurring or synthetic, and has a wide range of applications. The chemical structure and chemical, physical, and particulate characteristics of amorphous silica are given. Polymerization processes and further modifications and important applications, including silica sols and colloidal silica, silica gel, precipitated silica, pyrogenic silica (fumed silica), and fused silica and fused quartz, are discussed. Formation, properties, processing, modification approaches, and applications of naturally occurring amorphous silica are presented as well. Finally, health effects and safety issues of amorphous silica materials are introduced, including exposure and uptake, biological effects, and classification and labeling according to international regulations.

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Considerations for the Uptake Characteristic of Inorganic Nanoparticles into Mammalian Cells—Insights Gained by TEM Investigations

2018

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between individual uptake mechanisms for respective nanoparticle systems. Cells internalizing nanoparticles respond to the nanoparticles and different intracellular processes will be initiated, possibly resulting in the impairment of the cellular key functions. [4] Even though these investigations provide data with statistical relevance, they can merely provide an overall picture of the uptake mechanism, as issues of different uptake mechanisms in anisotropic nanoparticle suspensions are barely assessable. Simultaneously, the influence of the biological environment, e.g., the presence of serum proteins, salts, or altered pH conditions, may have a severe impact on the particle properties, which can entail various biological implications. As such, the role of high resolution imaging techniques, which provide a clear evidence for the nanoparticles' fate in the cellular environment, [5] their localization and targeting to different cellular organelles, as well as evidence for uptake and release mechanisms, have gained increasing importance. In particular, transmission electron microscopy (TEM) investigations provide attractive possibilities to study the interaction of particles with biological barriers, i.e., the extracellular membrane or the nuclear membrane, their accumulation into cellular organelles or their spatial distribution on the scale of individual nanoparticles [6] (e.g., uptake as individual nanoparticles or in form of large clusters, etc.). In addition TEM can simultaneously image also the cellular membranes and organelles to determine respective morphological changes [7] upon nanoparticle interaction. [8][9][10][11] Thus, attractive possibilities to acquire additional information on the particle fate inside the cell emerge from TEM investigations.It can be stated here, that the utilization of electron microscopy has been conducted during the last decades [5] and, in view of some extent electron microscopy investigations of particle uptake, can be regarded in this sense as a routinely applied technique. However, frequently TEM is only utilized to characterize the nanoparticle system or to prove particle internalization. Only a fraction of reported studies utilize TEM analysis to reveal details of nanoparticle uptake and internalization or even deduce uptake pathways from these studies. This might be related to the fact that sometimes the interpretation of TEM data is not straightforward but a number of experimental limitations have to be taken into account for such studies. In a recent review, the advantages and disadvantages of TEM investigations in the context of the The internalization of nanoparticles into mammalian cells relies on a complex interplay of several parameters, which enable and dictate uptake and fate of nanoparticles in the cellular system. Due to the complexity of the involved processes, a careful experimental design has to be developed to elucidate peculiarities of the uptake processes of new nanoparticle systems into cells. Individual parameters can be hardly considered alone but h...

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Time and space resolved uptake study of silicananoparticles by human cells

Cited by 215 publications

References 22 publications

Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells

Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells

Silica, Amorphous

Considerations for the Uptake Characteristic of Inorganic Nanoparticles into Mammalian Cells—Insights Gained by TEM Investigations

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