An investigation is conducted regarding the influence of surface roughness on the flashover strength of an insulator in vacuum. A series of experiments is first presented, showing the relationship between surface roughness and flashover voltage, combined with measurement of surface potential distribution. It is found that surface charging on a roughened dielectric is suppressed remarkably; also the flashover voltage threshold depicts a rise-and-fall trend with roughness increasing, exhibiting a voltage summit at a certain roughness value. In addition, optical observation is implemented to draw a parallel with an electron trajectory in vacuum. A proposal can therefore be made that multipactor expansion tends to be mitigated as electron bombardment on a dielectric occurs with a lower secondary electron yield. Then a theoretical model considering the microscopic physical process is established to explain the above phenomena, consisting of an internal charge migration layer, an interfacial electron absorption layer and an external electron obstruction layer. The validity of this theoretical model can be confirmed by obtaining the surface trap distribution, microscopic morphology and net secondary electron yield.