Time- and Cost-effective Production of Untagged Recombinant Mva by Flow Virometry and Direct Virus Sorting
Boselli Daniela,
Maddalena Panigada,
Simona Di Terlizzi
et al.
Abstract:Background
Recombinant MVAs (rMVAs) are widely used both in basic and clinical research. Our previously developed Red-to-Green Gene Swapping Method (RGGSM), a cytometry-based Cell-Sorting protocol, revolves around the transient expression of a green fluorescent cytoplasmic marker, to subsequently obtain purified untagged rMVA upon loss of that marker by site-specific recombination. The standard RGSSM is quite costly in terms of bench work, reagents, and Sorting Facility fees. Although faster than other method… Show more
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