Time and concentration dependency of MacroGard® induced apoptosis

Miest, Joanna J.; Hoole, D.

doi:10.1016/j.fsi.2014.11.009

Cited by 10 publications

(13 citation statements)

References 43 publications

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“…For various immunostimulating substances, potential negative effects on cellular homoeostasis have been addressed (Kepka, Verburg-van Kemenade, Homa & Chadzinska, 2014;Miest & Hoole, 2015). However, PHB did not affect the expression of the studied stress-and apoptosis-related genes (cat, hsp70, hsp90; casp3, casp9).…”

Section: Discussionmentioning

confidence: 99%

Immunostimulatory effects of dietary poly-β-hydroxybutyrate in European sea bass postlarvae

et al. 2017

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The stable production of high‐quality fry in marine aquaculture is still hampered by unpredictable mortality caused by infectious diseases during larval rearing. Consequently, the development of new biocontrol agents is crucial for a viable aquaculture industry. The bacterial energy storage compound poly‐β‐hydroxybutyrate (PHB) has been shown to exhibit beneficial properties on aquatic organisms such as enhanced survival, growth, disease resistance and a controlling effect on the gastrointestinal microbiota. However, the effect of PHB on the developing immune system of fish larvae has so far not been investigated. In this study, the effect of feeding PHB‐enriched Artemia nauplii on survival, growth and immune response in European sea bass (Dicentrarchus labrax) postlarvae was examined. Amorphous PHB was administered to 28‐day‐old sea bass postlarvae over a period of 10 days. The survival and growth performance were monitored, and the expression of 29 genes involved in immunity, growth, metabolism and stress‐response was measured. While the expression of the insulin‐like growth factor 1 (igf1), an indicator of relative growth, was upregulated in response to feeding PHB, the larval survival and growth performance remained unaffected. After 10 days of PHB treatment, the expression of the antimicrobial peptides dicentracin (dic) and hepcidin (hep) as well as mhc class IIa and mhc class IIb was elevated in the PHB fed postlarvae. This indicates that PHB is capable of stimulating the immune system of fish early life stages, which may be the cause of the increased resistance to diseases and robustness observed in previous studies.

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Section: Discussionmentioning

confidence: 99%

Immunostimulatory effects of dietary poly-β-hydroxybutyrate in European sea bass postlarvae

et al. 2017

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“…Since caspase is an indicator of apoptosis [25], this result indicated that β-glucans administered as supplementary diet to honeybees do not induce apoptosis. Previous studies on apoptosis were performed on fish using 1,3 β-glucans instead of 1,3-1,6 β-glucans; those elements could be the reason for the differences on apoptosis obtained in honeybees [23,24].…”

Section: Discussionmentioning

confidence: 99%

“…The response triggered by β-glucans can be remarkably different depending on the target organism and the dose administered [17,22]. Recent studies demonstrated that high concentration of 1,3 β-glucan is linked to the production of Reactive oxygen species (ROS) in carp leukocytes and the reduction of the immunostimulant effect inducing apoptosis in vitro and in vivo [23][24][25]. Caspase-3 (programmed cell death-activated endonuclease) is one of the key executioners of apoptosis [25].…”

Section: Introductionmentioning

confidence: 99%

Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)

Felicioli

Forzan

Sagona

et al. 2020

Veterinary Sciences

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Honeybee pathogens have an important role in honeybee colony mortality and colony losses; most of them are widely spread and necessitate worldwide solutions to contrast honeybee’s decline. Possible accepted solutions to cope with the spread of honeybee’s pathogens are focused on the study of experimental protocols to enhance the insect’s immune defenses. Honeybee’s artificial diet capable to stimulate the immune system is a promising field of investigation as ascertained by the introduction of 1,3-1,6 β-glucans as a dietary supplement. In this work, by collecting faecal samples of honeybees exposed to different dietary conditions of 1,3-1,6 β-glucans (0.5% and 2% w/w), it has been possible to investigate the Deformed wing virus (DWV) viral load kinetic without harming the insects. Virological data obtained by a one-step TaqMan RT-PCR highlighted the ability of 1,3-1,6 β-glucans to reduce the viral load at the 24th day of rearing. The results indicated that the diet supplemented with 1,3-1,6 β-glucans was associated with a dose-dependent activation of phenoloxidase. The control group showed a higher survival rate than the experimental groups. This research confirmed 1,3-1,6 β-glucans as molecules able to modulate honeybees’ defense pathways, and this is the first report in which the kinetic of DWV infection in honeybee faeces has been monitored by a RT-qPCR.

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“…Lower lysozyme concentrations were recorded at day 15 in fish of groups BG1-0.5 and BG2-0.5. Actually, effects of dietary β-glucan are time and dose dependent, that is, higher levels of inclusion associated to long periods of administration can actually result in reduction of immune response or even inactivation of immune system [31]. Although mechanism leading to this phenomenon are not yet clearly understood, studies have registered in vitro and in vivo leukocytes apoptosis [32] and induction of apoptosis-related genes [33] on glucan trials with fish.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulation of Juvenile Pacu, Piaractus mesopotamicus, by Different β(1-3)(1-6)-D glucan Products

Sabioni

Zanuzzo

Cyrino

2019

Braz. arch. biol. technol.

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Stress in intensive fish farming hamper immune function of fish and cause losses by disease outbreaks, a situation that can be minimized, but cannot be completely circumvented, by the use of immunomodulators. Addition of immunomodulators to aquafeeds has thus become a common practice. β-glucan (BG) is one of most studied and effective immunomodulators, aquaculture purposes included. Extracted from cell walls of bacteria, fungi and selected cereals, BG activity depends on the source and extraction methods. This study evaluated effects of two BG products (BG1 and BG2), extracted from Saccharomyces cerevisiae under varying extraction methods and with different immune activity, on the feeding of pacu Piaractus mesopotamicus juveniles. BG1 provided higher leukocytes respiratory activity when fed at 0.5% inclusion for 10 days and 0.1% inclusion for 15 days. Both products seems to cause negative effect on lysozyme concentration and monocytes profile when fed to pacu for 15 days at 0.5% inclusion. Although the results for HIGHLIGHTS • Two β-glucan products, with different production process, were tested in pacu feed. • Bacterial challenge and immunological assays was used to measure β-glucans activity in pacu. • A similar response was observed comparing a product with less β-glucan with the conventional.

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Time and concentration dependency of MacroGard® induced apoptosis

Cited by 10 publications

References 43 publications

Immunostimulatory effects of dietary poly-β-hydroxybutyrate in European sea bass postlarvae

Immunostimulatory effects of dietary poly-β-hydroxybutyrate in European sea bass postlarvae

Effect of Oral Administration of 1,3-1,6 β-Glucans in DWV Naturally Infected Newly Emerged Bees (Apis mellifera L.)

Immunomodulation of Juvenile Pacu, Piaractus mesopotamicus, by Different β(1-3)(1-6)-D glucan Products

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