Tiller Number1 encodes an ankyrin repeat protein that controls tillering in bread wheat

Dong, Caixia; Zhang, Lichao; Zhang, Qiang; Yang, Yuxin; Li, Danping; Xie, Zhencheng; Cui, Guoqing; Chen, Yaoyu; Wu, Lifen; Li, Zhan; Liu, Guoxiang; Zhang, Xueying; Liu, Cuimei; Chu, Jinfang; Zhao, Guangyao; Xia, Chuan; Jia, Jizeng; Sun, Jiaqiang; Kong, Xiuying; Liu, Xu

doi:10.1038/s41467-023-36271-z

Cited by 22 publications

(13 citation statements)

References 71 publications

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“…4), as reported by other studies in literature 46,83 . Additionally, the E-cadherin expression observed in epithelial cells covering the multiacinar cavity closely resembled the epithelial cell polarity observed in vivo 65,84 .…”

Section: Discussionsupporting

confidence: 66%

3Din vitromodeling of the exocrine pancreatic unit using tomographic volumetric bioprinting

Sgarminato

Madrid‐Wolff

Boniface

et al. 2023

Preprint

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Pancreatic ductal adenocarcinoma (PDAC) is the most frequent type of pancreatic cancer, one of the leading causes of cancer-related deaths worldwide. PDAC is marked by a dense, fibrous tumor microenvironment, in which stromal fibroblasts surround the cancerous ductal epithelial cells. The crosstalk between pancreatic cells and the surrounding fibroblasts leads to inflammation and stiffening of the surrounding tissue, which is believed to hinder anti-cancer drugs' uptake and effectiveness. In vitro, fully human models of the pancreatic cancer microenvironment are needed to foster the development of new, more effective therapies; but it is still challenging to make these models anatomically and functionally relevant. Here, we use tomographic volumetric bioprinting, a novel method to fabricate cell-laden viable constructs within less than a minute, to produce anatomically relevant fibroblast-laden gelatine methacrylate-based pancreatic models, including a duct and an acinus. We then seeded human pancreatic ductal epithelial (HPDE) cells, wild type or cancerous, into these models and evaluated the progression of the constructs for several days after printing. We show that the 3D pancreatic duct models remain viable for up to 14 days after bioprinting. We used immunofluorescence to evaluate the surrounding fibroblasts' activation by quantifying the relative expressions of alpha smooth muscle actin (αSMA) vs. actin in the fibroblasts. αSMA is known to be overexpressed in inflamed tumor-associated fibroblasts. We show that αSMA expression is significantly higher in fibroblasts co-cultured with cancerous than with wild-type HPDE cells, that this expression increases with time, and that it is higher in fibroblasts that lay closer to HPDE cells than in those deeper into the model. These models could potentially be used in drug development or to shed light on the early progression of the disease.

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Section: Discussionsupporting

confidence: 66%

3Din vitromodeling of the exocrine pancreatic unit using tomographic volumetric bioprinting

Sgarminato

Madrid‐Wolff

Boniface

et al. 2023

Preprint

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“…The plants that may perceive the functional loss of apical dominance via sugar and/or hormonal changes could produce tillers. Several phytohormones, such as auxin, cytokinin, strigolactone, and abscisic acid, may play important roles in apical tiller formation (Dong et al, 2023; Kotov et al, 2021). The polar auxin transport is known to block lateral growth.…”

Section: Discussionmentioning

confidence: 99%

“…Tillering is a complex process, consisting of initiation of the auxiliary meristem, bud formation, and tiller elongation (Wang et al, 2018). Previous studies show that tillering is controlled through complicated interactions among plant hormones, such as auxin, cytokinin, strigolactone, and abscisic acid (Dong et al, 2023; Kotov et al, 2021). Nevertheless, research on tiller formation in sorghum remains limited, especially under stress conditions (Chen et al, 2018; Govindarajulu et al, 2021; Kebrom & Mullet 2016).…”

Section: Introductionmentioning

confidence: 99%

The effects of heat stress on male reproduction and tillering in Sorghum bicolor

Smith,

Gentile,

Xin

et al. 2023

Food and Energy Security

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Due to global climate change, heat stress is increasingly harming the growth, development, overall biomass, and grain yield of numerous crops. Heat stress impairs pollen development and thus reduces seed set in Sorghum bicolor (L.) Moench; however, the effects of heat stress on anther development at specific stages and tiller formation remain incompletely understood. Here we report that exposure to heat stress [42°C/32°C (day/night)] at pollen mother cell (PMC) and booting stages profoundly disrupts tapetum and pollen development, resulting in a significant decrease in grain yield in sorghum. Sorghum plants subjected to 9 days or less of heat stress at the PMC stage exhibited normal pollen viability, but 12 days of heat stress caused almost complete loss of grain yield and the formation of nonviable pollen grains. Similarly, sorghum plants that were heat‐stressed for 3 days at the booting stage produced few seeds. Further analysis revealed that aberrant tapetum and pollen development contributed to the sterility of pollen in these heat‐stressed plants. Notably, in addition to inhibiting plant height, a 12‐day heat stress at the PMC stage promoted the formation of basal tillers, whereas a 3‐day heat stress at the booting stage stimulated the formation of apical tillers, which helped salvage seed yield under heat stress conditions. Moreover, the application of exogenous auxin promoted the formation of apical tillers and leaf numbers per apical tiller. Collectively, our findings suggest that sorghum is susceptible to heat stress during both early and late anther development, and auxin might be involved in governing the formation of apical tillers.

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“…A genetic complementation test and CRISPR/Cas9-mediated genome editing confirmed TN1 gene is responsible for the low-tillering phenotype of the tn1 mutant. (Dong et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Efficient cloning of genes for wheat yield component traits from QTLs via sequencing of the RIL population

Zhang,

Han,

Wang

et al. 2024

Preprint

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In wheat (Triticum aestivumL.), yield component traits (YCTs) are the most important yield traits. Only several genes for YCTs have been originally cloned. The efficient cloning of genes for YCTs directly from wheat remains a challenge. Here, we proposed a strategy for cloning genes from quantitative trait loci (QTLs) by sequencing of recombinant inbred lines (RILs) (QTL-Seq-RIL). Using the ‘TN18 × LM6’ RIL population as an example, we identified 138 candidate unigenes (CUGs) for YCTs from 77 stable QTLs. The average of CUGs per QTL was 1.8, which enabled us to confirm the CUGs directly. We have confirmed seven CUGs,TaIFABPL, TaDdRp, TaRLK, TaTD, TaTFC3, TaKMTandTaSPL17, via the CRISPR/Cas9 system. Of these, six genes were found firstly to regulate YCTs in crops except forTaSPL17. Five CUGs (includeTaSPL17) for which orthologous genes have been cloned previously with the same or similar agronomic functions. It is to say, 11 CUGs were preliminarily validated using a single RIL population. QTL-Seq-RIL provides an efficient method for rapid gene cloning using existing RIL populations.

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Tiller Number1 encodes an ankyrin repeat protein that controls tillering in bread wheat

Cited by 22 publications

References 71 publications

3Din vitromodeling of the exocrine pancreatic unit using tomographic volumetric bioprinting

3Din vitromodeling of the exocrine pancreatic unit using tomographic volumetric bioprinting

The effects of heat stress on male reproduction and tillering in Sorghum bicolor

Efficient cloning of genes for wheat yield component traits from QTLs via sequencing of the RIL population

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