TIIA attenuates LPS-induced mouse endometritis by suppressing the NF-κB signaling pathway

Abstract

Endometritis is one of the main diseases that harms the dairy cow industry. Tanshinone IIA (TIIA), a fat-soluble alkaloid isolated from Salviae miltiorrhizae, has been reported to have potent anti-inflammatory properties. However, the anti-inflammatory effects of TIIA on a mouse model of lipopolysaccharide (LPS)-induced endometritis remain to be elucidated. The purpose of the present study was to investigate the effects of TIIA on LPS-induced mouse endometritis. TIIA was intraperitoneally injected 1 h before and 12 h after perfusion of LPS into the uterus. A histological examination was then performed, and the concentrations of myeloperoxidase (MPO) and nitric oxide (NO) in the uterine tissue were determined. The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in a homogenate of the uterus were detected by enzyme-linked immunosorbent assay. The extent of phosphorylation of IκBα and p65 was detected by Western blotting. TIIA markedly reduced the infiltration of neutrophils, suppressed MPO activity and the concentration of NO, and attenuated the expression of TNF-α and IL-1β. Furthermore, TIIA inhibited the phosphorylation of the nuclear factor-kappa B (NF-κB) p65 subunit and the degradation of its inhibitor IκBα. All the results suggest that TIIA has strong anti-inflammatory effects on LPS-induced mouse endometritis.

The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway

“…Recently, study also showed the infiltration of neutrophils into the endometrium of mice with lipopolysaccharides (LPS)-induced endometritis [34]. When mass neutrophils reach the tissue at the site of infection, they will release abundant inflammatory factors, oxygen free radical and proteolytic enzyme to kill pathogens.…”

Baicalin Alleviates Lipopolysaccharide-Induced Liver Inflammation in Chicken by Suppressing TLR4-Mediated NF-κB Pathway

“…In recent years, isolating and developing naturally occurring monomers from medicinal plants have become resurgence of interest (Zheng et al, 2010 ). Furthermore, numerous Chinese herbal medicines have been widely used in clinical case, which are useful to treat inflammatory diseases, including mastitis, acute lung injury and endometritis (Lv et al, 2015 ).…”

Sodium tanshinone IIA sulfonate prevents lipopolysaccharide-induced inflammation via suppressing nuclear factor-κB signaling pathway in human umbilical vein endothelial cells

“…Sodium tanshinone IIA sulfonate (STS, molecular structure shown in Wu et al 2009), inhibit inflammatory response (Li et al 2015;Lu et al 2016;Yang et al 2016) and prevent atherosclerosis (Chang et al 2014;Chen et al 2012;Chen and Xu 2014;Fang et al 2008) as well as cardiac injury (Gao et al 2012;Jiang et al 2009) and hypertrophy (Feng and Zheng 2008;Weng et al 2015;Yang et al 2007). Tanshinone IIA has also been reported to prevent LPS-induced inflammation response in acute lung injury (Xu et al 2015) and in RAW264.7 macrophages (Fan et al 2016;Jang et al 2006) via inhibition of the NF-κB pathway.…”