Tightly controlled expression of OsbHLH35 is critical for anther development in rice

Ortolan, Francieli; Fonini, Leila Spagnolo; Pastori, Tamara; Mariath, Jorge Ernesto de Araújo; Saibo, Nelson J. M.; Margis‐Pinheiro, Márcia; Lazzarotto, Fernanda

doi:10.1016/j.plantsci.2020.110716

Cited by 11 publications

(6 citation statements)

References 53 publications

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“…Some canonical and novel miRNA-TF regulation patterns that participated in flower development were identified, such as lch-miR156/157-SPLs/SBPs, Novel104/113/401-SPLs, lch-miR159a/c-GAMYBs, Novel32/394-GAMYB, lch-miR167a-ARF6, lch-miR172e-AP2, Novel107-AP2, Novel3-bHLH35, and Novel301-bHLH62 (Figure 9). These miRNA-targeted TFs are involved in floral organ morphogenesis, floral organ maturation, and flowering time regulation (Wu et al, 2006;Wollmann et al, 2010;Li et al, 2013;Xu et al, 2016;Ding et al, 2020;Ortolan et al, 2021;Zhou et al, 2021). Although these miRNAs were involved in flower development, their expression patterns were different owing to their different roles.…”

Section: Analysis Of Microrna-transcription Factor Regulatory Patterns In Liriodendron Chinensementioning

confidence: 99%

“…For example, lch-miR157a and Novel3 miRNA were strongly expressed in the stamen, whereas lch-miR159a and lch-miR159c were weakly expressed in the stamen (Figures 4A-C,S). The high expression of Novel3 in the stamen would inevitably lead to the low expression of bHLH35 in the stamen, which is essential for anthers development (Ortolan et al, 2021). During anther development, GAMYBs expression levels increased as miR159 levels decreased; therefore, the low expression level of lch-miR159a/c in stamens may be essential for anther development (Tsuji et al, 2006).…”

Section: Analysis Of Microrna-transcription Factor Regulatory Patterns In Liriodendron Chinensementioning

confidence: 99%

“…Moreover, Novel3-bHLH35, lch-miR159a/c-GAMYBs, and lch-miR167a-ARF6 may be related to male and female fertility. In rice, the overexpression of bHLH35 resulted in small and curved anthers, and miR159 targeted GAMYB to regulate anther development (Tsuji et al, 2006;Ortolan et al, 2021). In A. thaliana, miR167 targets ARF6 to control female and male reproduction (Wu et al, 2006).…”

Section: Liriodendron Chinensementioning

confidence: 99%

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The Roles of microRNA-Long Non-coding RNA-mRNA Networks in the Regulation of Leaf and Flower Development in Liriodendron chinense

Xia

Yang

et al. 2022

Front. Plant Sci.

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The leaf and the flower are vital plant organs owing to their roles in photosynthesis and reproduction. Long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and transcription factors (TFs) are very important to the development of these organs. Liriodendron chinense is a common ornamental tree species in southern China with an unusual leaf shape and tulip-like flowers. The genetic mechanisms underlying leaf and flower development in L. chinense and the miRNA-lncRNA-TF regulatory networks are poorly studied. Through the integration and analysis of different types of sequencing data, we identified the miRNA-lncRNA-TF regulatory networks that were related to leaf and flower development. These networks contained 105 miRNAs, 258 lncRNAs, 393 TFs, and 22 endogenous target mimics. Notably, lch-lnc7374-miR156h-SPL3 and lch-lnc7374-miR156j-SPL9 were potential regulators of stamen and pistil development in L. chinense, respectively. miRNA-lncRNA-mRNA regulatory networks were shown to impact anther development, male and female fertility, and petal color by regulating the biosynthesis of phenylpropanoid metabolites. Phenylpropanoid metabolite biosynthesis genes and TFs that were targeted by miRNAs and lncRNAs were differentially expressed in the leaf and flower. Moreover, RT-qPCR analysis confirmed 22 differentially expressed miRNAs, among which most of them showed obvious leaf or flower specificity; miR157a-SPL and miR160a-ARF module were verified by using RLM-RACE, and these two modules were related to leaf and flower development. These findings provide insight into the roles of miRNA-lncRNA-mRNA regulatory networks in organ development and function in L. chinense, and will facilitate further investigation into the regulatory mechanisms of leaf and flower development in L. chinense.

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Section: Analysis Of Microrna-transcription Factor Regulatory Patterns In Liriodendron Chinensementioning

confidence: 99%

Section: Analysis Of Microrna-transcription Factor Regulatory Patterns In Liriodendron Chinensementioning

confidence: 99%

Section: Liriodendron Chinensementioning

confidence: 99%

See 1 more Smart Citation

The Roles of microRNA-Long Non-coding RNA-mRNA Networks in the Regulation of Leaf and Flower Development in Liriodendron chinense

Xia

Yang

et al. 2022

Front. Plant Sci.

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“…The bHLH protein is the most extensive class of transcription factors in eukaryotes, which can regulate gene expression through interaction with specific motif in target genes. The bHLH TF is not only universally involved in plant growth and metabolism, but also plays an important role in plant response to stress ( He et al, 2021 ; Ortolan et al, 2021 ; Qiu et al, 2020 ; Sun, Wang & Sui, 2018 ). However, little is known about bHLH protein in tung tree.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and transcriptional profiling of the basic helix-loop-helix gene family in tung tree (Vernicia fordii)

Liu

Zhuang

et al. 2022

PeerJ

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The basic helix-loop-helix (bHLH) transcription factor gene family is one of the largest gene families and is extensively involved in plant growth, development, biotic and abiotic stress responses. Tung tree (Vernicia fordii) is an economically important woody oil plant that produces tung oil rich in eleostearic acid. However, the characteristics of the bHLH gene family in the tung tree genome are still unclear. Hence, VfbHLHs were first searched at a genome-wide level, and their expression levels in various tissues or under low temperature were investigated systematically. In this study, we identified 104 VfbHLHs in the tung tree genome, and these genes were classified into 18 subfamilies according to bHLH domains. Ninety-eight VfbHLHs were mapped to but not evenly distributed on 11 pseudochromosomes. The domain sequences among VfbHLHs were highly conserved, and their conserved residues were also identified. To explore their expression, we performed gene expression profiling using RNA-Seq and RT-qPCR. We identified five, 18 and 28 VfbHLH genes in female flowers, male flowers and seeds, respectively. Furthermore, we found that eight genes (VfbHLH29, VfbHLH31, VfbHLH47, VfbHLH51, VfbHLH57, VfbHLH59, VfbHLH70, VfbHLH72) were significant differential expressed in roots, leaves and petioles under low temperature stress. This study lays the foundation for future studies on bHLH gene cloning, transgenes, and biological mechanisms.

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“…Furthermore, rice TIP2 and EAT1 belong to subfamily II, which indicate that the bHLH genes in subfamily II may act as the key transcriptional regulators in anther development ( Table 1 ). The overexpression of OsbHLH35 endows plants with small and curved anthers, which result in a reduction in the seed production ( Figure 2 ) [ 71 ]. In this process, three Growth Regulating Factor (GRF) family members, including OsGRF3, OsGRF4, and OsGRF11, act as the transcriptional regulators of OsbHLH35 , and OsGRF11 acts as a negative regulator of OsbHLH35 in rice.…”

Section: The Bhlh Transcription Factor Family In Plantsmentioning

confidence: 99%

Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

Zuo

Lee

Kang

2023

IJMS

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Plant basic helix-loop-helix (bHLH) transcription factors are involved in many physiological processes, and they play important roles in the abiotic stress responses. The literature related to genome sequences has increased, with genome-wide studies on the bHLH transcription factors in plants. Researchers have detailed the functionally characterized bHLH transcription factors from different aspects in the model plant Arabidopsis thaliana, such as iron homeostasis and abiotic stresses; however, other important economic crops, such as rice, have not been summarized and highlighted. The bHLH members in the same subfamily have similar functions; therefore, unraveling their regulatory mechanisms will help us to identify and understand the roles of some of the unknown bHLH transcription factors in the same subfamily. In this review, we summarize the available knowledge on functionally characterized bHLH transcription factors according to four categories: plant growth and development; metabolism synthesis; plant signaling, and abiotic stress responses. We also highlight the roles of the bHLH transcription factors in some economic crops, especially in rice, and discuss future research directions for possible genetic applications in crop breeding.

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Tightly controlled expression of OsbHLH35 is critical for anther development in rice

Cited by 11 publications

References 53 publications

The Roles of microRNA-Long Non-coding RNA-mRNA Networks in the Regulation of Leaf and Flower Development in Liriodendron chinense

The Roles of microRNA-Long Non-coding RNA-mRNA Networks in the Regulation of Leaf and Flower Development in Liriodendron chinense

Genome-wide identification and transcriptional profiling of the basic helix-loop-helix gene family in tung tree (Vernicia fordii)

Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses

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