Tight junction between endothelial cells: the interaction between nanoparticles and blood vessels

Zhang, Yue; Yang, Wan-Xi

doi:10.3762/bjnano.7.60

Cited by 36 publications

(14 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NPs may also transport across ECs to targets via exocytosis (Oh and Park, ; Frohlich, ) or paracellular pathways through the regulation of junction proteins (Fig. ) (Zhang and Yang, ). Exocytosis by HUVECs has been observed after exposure to mesoporous silica NPs (Slowing et al, ), peptide functionalized Au NPs (Bartczak et al, ) and silicon QD (Ohta et al, ).…”

Section: Interactions Between Nps and Huvecsmentioning

confidence: 99%

The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review

Cao

Gong

Liu

et al. 2017

J of Applied Toxicology

229

173

View full text Add to dashboard Cite

With the rapid development of nanotechnologies, nanoparticles (NPs) are increasingly produced and used in many commercial products, which could lead to the contact of human blood vessels with NPs. Thus, it is necessary to understand the adverse effects of NPs to relevant cells lining human blood vessels, especially endothelial cells (ECs) that cover the lumen of blood vessels. Human umbilical vein endothelial cells (HUVECs) are among one of the most popular models used for ECs in vitro. In the present review, we discussed studies that have used HUVECs as a model to investigate the EC-NP interactions, the toxic effects of NPs on ECs and the mechanisms. The results of these studies indicated that NPs could be internalized into HUVECs by the endocytosis pathway as well as transported across HUVECs by exocytosis and paracellular pathways. Exposure of HUVECs to NPs could induce cytotoxicity, genotoxicity, eNOS uncoupling and endothelial activation, which could be explained by NP-induced oxidative stress, inflammatory response and dysfunction of organelles. In addition, some studies have also evaluated the influences of microenvironment (e.g. the presence of proteins and excessive nutrients), the physiological and/or pathological stimuli related to the diversity of ECs (e.g. shear stress, cyclic stretch and inflammatory stimuli), and the physicochemical properties of NPs on the responses of ECs to NP exposure. In conclusion, it has been suggested that HUVECs could be considered as a relatively reliable and simple in vitro model for ECs to predict and evaluate the toxicity of NPs to endothelium. Copyright © 2017 John Wiley & Sons, Ltd.

show abstract

Section: Interactions Between Nps and Huvecsmentioning

confidence: 99%

The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review

Cao

Gong

Liu

et al. 2017

J of Applied Toxicology

229

173

View full text Add to dashboard Cite

show abstract

“…[21][22][23] Among these, endothelial cells constitute one of the very first barriers that nanomedicines need to overcome in order to reach the targeted organ following administration into the blood stream. 24,25 In order to control access to the underlying tissue and impair the passage of pathogens and undesired molecules, endothelial cells are typically organized in cellular barriers expressing tight junctions among cells, and with a clear spatial and functional separation between their apical and basolateral membranes. 26 As a result, endocytic pathways are differentially expressed between the two sides of the cell monolayer.…”

Section: Introductionmentioning

confidence: 99%

Effect of the development of a cell barrier on nanoparticle uptake in endothelial cells

2018

View full text Add to dashboard Cite

In order to improve the current success of nanomedicine, a better understanding of how nano-sized materials interact with and are processed by cells is required. Typical in vitro nanoparticle-cell interaction studies often make use of cells cultured at different cell densities. However, in vivo, for their successful delivery to the target tissue, nanomedicines need to overcome several barriers, such as endothelial and epithelial cell barriers. Unlike sub-confluent or confluent cell cultures, cell barriers are tight cell monolayers, expressing a series of specialized tight junction proteins between adjacent cells to limit paracellular transport and ensure close cell-to-cell interactions. A clear understanding on how the development of cells into a cell barrier may affect the uptake of nano-sized drug carriers is still missing. To this aim, here, human primary umbilical vein endothelial cells (HUVEC) are used as a model cell line to form endothelial cell barriers. Then, nanoparticle uptake is assessed in the developed endothelial barriers and compared to the uptake in sub-confluent or confluent HUVEC cultures. The results clearly show that the organization of cells into a cell barrier leads to a differential gene expression of endocytic markers, and - interestingly - this is accompanied by reduced nanoparticle uptake levels. Transport inhibitors are used to characterise the mechanisms involved in the uptake. However, we show that some of them can strongly compromise barrier integrity, thus impairing the interpretation of the outcomes, and overall, only a partial inhibition of nanoparticle uptake could be obtained.

show abstract

“…They can also induce inflammation and cytotoxicity in endothelial cells through the activation of potassium channels [103]. Moreover, it has been suggested that nanoparticles can regulate the barrier function of tight junction (occludins, claudins, and ZO proteins) because they interact with key kinases, altering not only the oxidative status around the junction between endothelial cells but also altering the blood flow into the vessel [110]. These data indicate that nanoparticle/endothelial cell interaction can modify the function of the blood vessel, whether in the site of injection at the moment of intravenous administration, during the distribution process, or when directed toward specific targets.…”

Section: Interaction Of Nanomaterials With the Vascular Endotheliummentioning

confidence: 99%

Interaction of Nanoparticles with Blood Components and Associated Pathophysiological Effects

Cruz¹,

Rodríguez-Fragoso²,

JorgeReyes-Esparza³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

View full text Add to dashboard Cite

Nanotechnology currently plays a pivotal role in several fields and has enabled substantial advances in a relatively short time. In biomedicine, nanomaterials can be potentially employed as a tool for early diagnosis and an innovative mode of drug delivery. Novel nanomaterials are currently widely manipulated without a full assessment of their potential health risks. It is commonly thought that nanomaterials' first contact with the organism is through the different components of the immune system. However, if the entry route is intravenous, the first contact will be with the blood's components (erythrocytes, platelets, white cells, plasma and complement proteins). The presence of nanomaterials within a dynamic environment such as the bloodstream can produce potential harmful effects following interaction with several blood components. The design of innovative strategies leading to the development of more hemocompatible nanomaterials is also necessary.

show abstract

Tight junction between endothelial cells: the interaction between nanoparticles and blood vessels

Cited by 36 publications

References 103 publications

The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review

The use of human umbilical vein endothelial cells (HUVECs) as an in vitro model to assess the toxicity of nanoparticles to endothelium: a review

Effect of the development of a cell barrier on nanoparticle uptake in endothelial cells

Interaction of Nanoparticles with Blood Components and Associated Pathophysiological Effects

Contact Info

Product

Resources

About