Tight gene co-expression in BCB positive cattle oocytes and their surrounding cumulus cells

Walker, Bailey N.; Nix, Jada; Wilson, Chace; Marrella, Mackenzie A.; Speckhart, Savannah L.; Wooldridge, Lydia K.; Yen, Con‐Ning; Bodmer, Jocelyn S.; Kirkpatrick, Laila T.; Moorey, Sarah E.; Gerrard, David E.; Ealy, Alan D.; Biase, Fernando H.

doi:10.1186/s12958-022-00994-3

Cited by 8 publications

(3 citation statements)

References 83 publications

(133 reference statements)

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“…A recently published transcriptomic dataset [ 26 ] from bovine cumulus cells and denuded oocytes harvested immediately after COC retrieval from slaughterhouse-derived ovaries (i.e., not matured) was used to examine the presence or absence of expression for IL6 , IL11 , and LIF, and their receptors ( Table 2 ). No IL6 , IL11 , or LIF transcripts were detected in the oocyte and cumulus cells, but transcripts for their receptors was detected.…”

Section: Resultsmentioning

confidence: 99%

Influences of Supplementing Selective Members of the Interleukin-6 Cytokine Family on Bovine Oocyte Competency

McKinley,

Speckhart,

Keane

et al. 2023

Animals

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This work explored whether supplementing selective members of the interleukin-6 (IL6) cytokine family during in vitro bovine oocyte maturation affects maturation success, cumulus–oocyte complex (COC) gene expression, fertilization success, and embryo development potential. Human recombinant proteins for IL6, IL11, and leukemia inhibitory factor (LIF) were supplemented to COCs during the maturation period, then fertilization and embryo culture commenced without further cytokine supplementation. The first study determined that none of these cytokines influenced the rate that oocytes achieved arrest at meiosis II. The second study identified that LIF and IL11 supplementation increases AREG transcript abundance. Supplementation with IL6 supplementation did not affect AREG abundance but reduced HAS2 transcript abundance. Several other transcriptional markers of oocyte competency were not affected by any of the cytokines. The third study determined that supplementing these cytokines during maturation did not influence fertilization success, but either LIF or IL11 supplementation increased blastocyst development. No effect of IL6 supplementation on subsequent blastocyst development was detected. The fourth experiment explored whether each cytokine treatment affects the post-thaw survivability of cryopreserved IVP blastocysts. None of the cytokines supplemented during oocyte maturation produced any positive effects on post-thaw blastocyst re-expansion and hatching. In conclusion, these outcomes implicate IL11 and LIF as potentially useful supplements for improving bovine oocyte competency.

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Section: Resultsmentioning

confidence: 99%

Influences of Supplementing Selective Members of the Interleukin-6 Cytokine Family on Bovine Oocyte Competency

McKinley,

Speckhart,

Keane

et al. 2023

Animals

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“…Indeed, inhibition of CDC5L by siRNA and antibody microinjection impaired porcine oocyte maturation. More recently, BCB stained bovine oocytes at the mature metaphase II stage have been investigated by single-cell RNAseq, but there were no significant differences in gene expression profiles between BCB+ and BCB− oocytes [ 34 ]. Therefore, it is still largely unknown why mature metaphase II BCB− oocytes have low competency and how mtDNA deficiency contributes to oocyte quality, especially at the transcript level and whether the mitochondrial genome can influence the oocyte’s nuclear genome.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA Deficiency and Supplementation in Sus scrofa Oocytes Influence Transcriptome Profiles in Oocytes and Blastocysts

Okada

McIlfatrick

John

2023

IJMS

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Mitochondrial DNA (mtDNA) deficiency correlates with poor oocyte quality and fertilisation failure. However, the supplementation of mtDNA deficient oocytes with extra copies of mtDNA improves fertilisation rates and embryo development. The molecular mechanisms associated with oocyte developmental incompetence, and the effects of mtDNA supplementation on embryo development are largely unknown. We investigated the association between the developmental competence of Sus scrofa oocytes, assessed with Brilliant Cresyl Blue, and transcriptome profiles. We also analysed the effects of mtDNA supplementation on the developmental transition from the oocyte to the blastocyst by longitudinal transcriptome analysis. mtDNA deficient oocytes revealed downregulation of genes associated with RNA metabolism and oxidative phosphorylation, including 56 small nucleolar RNA genes and 13 mtDNA protein coding genes. We also identified the downregulation of a large subset of genes for meiotic and mitotic cell cycle process, suggesting that developmental competence affects the completion of meiosis II and first embryonic cell division. The supplementation of oocytes with mtDNA in combination with fertilisation improves the maintenance of the expression of several key developmental genes and the patterns of parental allele-specific imprinting gene expression in blastocysts. These results suggest associations between mtDNA deficiency and meiotic cell cycle and the developmental effects of mtDNA supplementation on Sus scrofa blastocysts.

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“…BCB staining is a reliable method for selecting cumulus–oocyte complexes (COCs) without adversely affecting oocyte quality in mammals, such as mice, cattle, goats, and pigs [ 5 ]. Multiple molecular and physical differences exist between BCB+ and BCB− oocytes, such as diameter, lipid content, mitochondrial function, and gene expression [ 6 ]. Notably, BCB+ oocytes exhibit significantly higher in vitro embryo development than BCB− oocytes, indicating that the quality of BCB− oocytes is lower than that of BCB+ oocytes [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Restoration of Developmental Competence in Low-Quality Porcine Cumulus–Oocyte Complexes through the Supplementation of Sonic Hedgehog Protein during In Vitro Maturation

Jeong

Kang

Song

et al. 2023

Animals

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The sonic hedgehog (SHH) pathway is an important signaling pathway for mammalian ovarian folliculogenesis and oocyte maturation. A previous study demonstrated that low-quality porcine cumulus–oocyte complexes (COCs) have low developmental competence, with lower SHH signaling protein expression before and after in vitro maturation (IVM) than high-quality COCs. However, there is no reported evidence on the restorative effects of SHH protein supplementation during the IVM of low-quality porcine COCs. Therefore, this study investigated the effects of SHH protein supplementation on the IVM of low-quality porcine COCs, as assessed by brilliant cresyl blue (BCB) staining. To examine this, we designed four groups: (i) BCB− (low-quality), (ii) BCB− + SHH, (iii) BCB+ (high-quality), and (iv) BCB+ + SHH. While the supplementation of SHH protein with high-quality COCs had no effect, supplementation with low-quality COCs significantly improved cumulus cell expansion, metaphase II rate, and subsequent embryo development following parthenogenetic activation. Our results provide the first evidence that the low developmental competence of low-quality porcine COCs can be improved by supplementation with the SHH protein. These results indicate that an active SHH signaling pathway is required for the acquisition of developmental competence in porcine COCs.

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Tight gene co-expression in BCB positive cattle oocytes and their surrounding cumulus cells

Cited by 8 publications

References 83 publications

Influences of Supplementing Selective Members of the Interleukin-6 Cytokine Family on Bovine Oocyte Competency

Influences of Supplementing Selective Members of the Interleukin-6 Cytokine Family on Bovine Oocyte Competency

Mitochondrial DNA Deficiency and Supplementation in Sus scrofa Oocytes Influence Transcriptome Profiles in Oocytes and Blastocysts

Restoration of Developmental Competence in Low-Quality Porcine Cumulus–Oocyte Complexes through the Supplementation of Sonic Hedgehog Protein during In Vitro Maturation

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