Tight Control of Matrix Metalloproteinase-1 Activity in Human Skin¶

Südel, Kirstin M.; Venzke, Kirsten; Knußmann-Hartig, Elke; Moll, Ingrid; Stäb, Franz; Wenck, Horst; Wittern, Klaus‐Peter; Gercken, Günther; Gallinat, Stefan

doi:10.1562/0031-8655(2003)078<0355:tcomma>2.0.co;2

Cited by 42 publications

(33 citation statements)

References 21 publications

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“…We observed that MMP-1 mRNA expression was activated within 24 h after UVB irradiation followed by a decline to the level in cells (not shown data). Südel et al 2) reported that after UV irradiation, MMP-1 was time-dependently induced on the mRNA level in dermal fibroblasts in vitro/in vivo in human skin and then a strong induction of MMP-1 mRNA at 24 h after UV irradiation as similar as our results. We also found that UVB-induced MMP-1 mRNA expression showed a negative relationship with fucoidan levels followed by a 24 h incubation period.…”

Section: Discussionsupporting

confidence: 91%

“…Südel et al 2) suggested that time after UV exposure in vitro directly determines the extent of damage that is inflicted on MMP-1 expression. Di Girolamo et al 18) reported that MMP-1 expression increases both as a function of time and dose in cultured cells in response to UV exposure.…”

Section: Discussionmentioning

confidence: 99%

“…The photoaging process increases skin fragility, laxity, blister formation, leathery appearance and formation of wrinkles. 2) UVB induces the production of matrix metalloproteinases (MMPs) by activating cellular signaling transduction pathways 3) ; MMPs are responsible for the degradation or synthesis inhibition of collagenous extracellular matrix in connective tissues.…”

Section: )mentioning

confidence: 99%

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Fucoidan Inhibits UVB-Induced MMP-1 Expression in Human Skin Fibroblasts

Moon

Lee

Shim

et al. 2008

Biological & Pharmaceutical Bulletin

119

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Skin can age in two ways: (i) intrinsic or chronologic aging, which is the process of senescence that affects all body organs; and (ii) extrinsic aging which occurs as a consequence of exposure to environmental factors including sunlight and cigarette smoke. 1)The most important of these factors is sunlight, particularly exposure to ultraviolet (UV)B irradiation, which causes photoaging. The photoaging process increases skin fragility, laxity, blister formation, leathery appearance and formation of wrinkles. 2)UVB induces the production of matrix metalloproteinases (MMPs) by activating cellular signaling transduction pathways 3) ; MMPs are responsible for the degradation or synthesis inhibition of collagenous extracellular matrix in connective tissues.3) Collagen represents the main component of the extracellular matrix of dermal connective tissue, and its concentration decreases in chronoaging and photoaging.All matrix macromolecules in the dermis can be digested by MMPs acting alone or in combination. The MMPs form a family of structurally and functionally related zinc endopeptidases that exhibit various substrate specificities. 4) Once collagen is initially cleaved by MMP-1, MMP-3 and other MMPs, collagen breakdown is further promoted. The enzyme mainly responsible for collagen breakdown in skin is, MMP-1 (fibroblast collagenase), which cleaves type I, III, VII, VIII and X collagen.Human fibroblast collagenase was the first vertebrate collagenase both purified to homogeneity as a protein, and cloned as a cDNA. This enzyme has been designated as matrix metalloproteinase-1 (MMP-1) and has served as the prototype for all the interstitial collagenases. MMP-1 is also known as collagenase-1. Starting from the N terminus the following features of domain organization are observed. 5)The pre-domain specifies a signal rich in hydrophobic amino acids that destines the synthesized polypeptide to the endoplasmic reticulum where it is removed during the transportation of the molecule from the cell to the outside. The propeptide domain indicates a sequence that is responsible of keeping the pro-form inactive. It presents a cysteine residue 73 that is located in a conserved sequence PRCGVPD opposite to a zinc atom at the active-form site and coordinated to it through an -SH group. The enzymatic activity of the proform enzyme is turned on by the displacement of this cysteine residue ("cysteine switch") and occurs by proteolytic cleavage, or by chemical disruption as in the case of oxidation or treatment with mercurial compounds. 6)Recent studies have shown that hairless mice exposed to UVB developed wrinkled skin and significantly enhanced MMP-1 mRNA expression. However, they have shown that the inihibition of MMP-1 activities by a specific MMP inhibitor suppresses UVB-induced wrinkle formation.7) This evidence suggests that MMP-1 plays a major role in the process of photoaging.Brown seaweed has been a staple of both Korean and Japanese diets and has also been documented as being used in traditional Chinese medicine for over ...

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: )mentioning

confidence: 99%

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Fucoidan Inhibits UVB-Induced MMP-1 Expression in Human Skin Fibroblasts

Moon

Lee

Shim

et al. 2008

Biological & Pharmaceutical Bulletin

119

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“…Suction blisters were obtained from the volar forearms of 10 healthy male volunteers, as described previously [40]. Suction blister roofs were taken and immediately stored at −20°C.…”

Section: Methodsmentioning

confidence: 99%

Aging and Chronic Sun Exposure Cause Distinct Epigenetic Changes in Human Skin

et al. 2010

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Epigenetic changes are widely considered to play an important role in aging, but experimental evidence to support this hypothesis has been scarce. We have used array-based analysis to determine genome-scale DNA methylation patterns from human skin samples and to investigate the effects of aging, chronic sun exposure, and tissue variation. Our results reveal a high degree of tissue specificity in the methylation patterns and also showed very little interindividual variation within tissues. Data stratification by age revealed that DNA from older individuals was characterized by a specific hypermethylation pattern affecting less than 1% of the markers analyzed. Interestingly, stratification by sun exposure produced a fundamentally different pattern with a significant trend towards hypomethylation. Our results thus identify defined age-related DNA methylation changes and suggest that these alterations might contribute to the phenotypic changes associated with skin aging.

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“…For UV radiation, an Oriel solar simulator (Oriel Instruments, Stratford, CT, USA, 1000 W UV Solar Simulator, 8 · 8 inch beam size, Model: 91294, Newport, linaer UV spectrum Fig. Suction blister fluids that have been proven as a valuable source for the study of soluble biomarkers (22,23) were raised immediately and 24 h after irradiation, as described (24)(25)(26). The individual minimal erythema dose (MED) was determined in a defined area on the volar forearms (2 · 4 cm).…”

Section: Generation Of Suction Blister Fluidsmentioning

confidence: 99%

UV radiation induces the release of angiopoietin‐2 from dermal microvascular endothelial cells

Navid

Kolbe

Stäb

et al. 2011

Experimental Dermatology

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In human skin, ultraviolet radiation (UVR)-induced erythema is characterized by the inflammatory and angiogenic activation of dermal endothelial cells. Recently, it has been shown that the release of angiopoietin-2 (Ang-2) from cytoplasmic storages of activated endothelial cells is crucial for the induction of inflammation and angiogenesis. Therefore, we hypothesized that UVR exposure induces the release of Ang-2 from endothelial cells controlling the early steps of erythema formation. In an in vivo study, suction blister fluids generated from UV-irradiated skin showed significantly increased concentrations of Ang-2, vascular endothelial growth factor (VEGF) and tumor necrosis factor-α (TNFα). Likewise, in vitro UVR exposure of human dermal microvascular endothelial cells (HDMECs) triggered the release of Ang-2 that enhanced the pro-inflammatory response of these cells and facilitated their detachment from smooth muscle cells as evidenced by employing a three-dimensional co-culture spheroid model. These effects were inhibited by angiopoietin-1 (Ang-1), which competes with Ang-2 for binding the endothelial cell Tie2 receptor. Collectively, these observations suggest that UVR triggers the release of endothelial Ang-2 which may promote the destabilization and pro-inflammatory phenotype of the microvascular endothelium. As Ang-1 counteracts UVR-induced effects, stimulating the Ang-1 activity may represent a strategy to stabilize the dermal microcirculatory system, thus protecting against UVR-induced skin damages.

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Tight Control of Matrix Metalloproteinase-1 Activity in Human Skin¶

Cited by 42 publications

References 21 publications

Fucoidan Inhibits UVB-Induced MMP-1 Expression in Human Skin Fibroblasts

Fucoidan Inhibits UVB-Induced MMP-1 Expression in Human Skin Fibroblasts

Aging and Chronic Sun Exposure Cause Distinct Epigenetic Changes in Human Skin

UV radiation induces the release of angiopoietin‐2 from dermal microvascular endothelial cells

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