Tight control of growth and cell differentiation in photoautotrophically growing moss (Physcomitrella patens) bioreactor cultures

Hohe, Annette; Decker, Eva L.; Gorr, Gilbert; Schween, Gabriele; Reski, Ralf

doi:10.1007/s00299-002-0463-y

Cited by 71 publications

(39 citation statements)

References 15 publications

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“…In contrast to vascular plants, where autotrophy is usually only achieved with elevated levels of CO 2 (Kozai 1991) and increased light levels, many bryophyte species can grow autotrophically (Takami et al 1988;Hohe et al 2002a;Rowntree 2006). Therefore, addition of sucrose to bryophyte cultures is not strictly necessary.…”

Section: In Vitro Culture Of Bryophytesmentioning

confidence: 91%

In vitro conservation of European bryophytes

Rowntree

Pressel

Ramsay

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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The use of in vitro techniques for conservation has been rising steadily since their inclusion in The Convention on Biological Diversity and The Global Strategy for Plant Conservation. Unfortunately, bryophytes are often overlooked in conservation initiatives, but they are important in a number of large-scale ecosystem processes, i.e. nutrient, water and carbon cycling. There is a long history of the use of tissue culture in cultivating bryophytes, and many species respond well to in vitro techniques. For 6 yr (2000)(2001)(2002)(2003)(2004)(2005)(2006), The Royal Botanic Gardens, Kew and the UK statutory conservation agencies supported a project for the ex situ conservation of bryophytes. Living and cryopreserved collections of UK threatened species were successfully established and the cryopreserved collection continues to be maintained. Other in vitro conservation collections are maintained over Europe, at botanic gardens, museums and by individual university researchers, but there is no coherent European collection of bryophytes for conservation, or standardisation of techniques. A major issue for many in vitro collections is the maintenance of within species genetic diversity. Such diversity is considered to be important, as it is the basis by which populations of species can adapt to new conditions and evolve. We are proposing to establish a European network for in vitro conservation of bryophytes. We envisage that this will include living collections, cryopreserved collections and spore collections. Conservation of genetic diversity would be a priority and the collections would provide a valuable resource for conservation initiatives and support research into rare and threatened species.

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Section: In Vitro Culture Of Bryophytesmentioning

confidence: 91%

In vitro conservation of European bryophytes

Rowntree

Pressel

Ramsay

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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“…was cultured as protonemal tissue, either on agar medium overlain with cellophanes (15) (Leeds) or in liquid culture (16) (Freiburg). The techniques for protoplast isolation, transformation and regeneration of stably transformed plants have been described previously (4,17–19). …”

Section: Methodsmentioning

confidence: 99%

The mechanism of gene targeting in Physcomitrella patens: homologous recombination, concatenation and multiple integration

Kamisugi

Schlink

Rensing

et al. 2006

Nucleic Acids Research

123

109

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The model bryophyte Physcomitrella patens exhibits high frequencies of gene targeting when transformed with DNA constructs containing sequences homologous with genomic loci. ‘Targeted gene replacement’ (TGR) resulting from homologous recombination (HR) between each end of a targeting construct and the targeted locus occurs when either single or multiple targeting vectors are delivered. In the latter instance simultaneous, multiple, independent integration of different transgenes occurs at the targeted loci. In both single gene and ‘batch’ transformations, DNA can also be found to undergo ‘targeted insertion’ (TI), integrating at one end of the targeted locus by HR with one flanking sequence of the vector accompanied by an apparent non-homologous end-joining (NHEJ) event at the other. Untargeted integration at nonhomologous sites also occurs, but at a lower frequency. Molecular analysis of TI at a single locus shows that this occurs as a consequence of concatenation of the transforming DNA, in planta, prior to integration, followed by HR between a single site in the genomic target and two of its repeated homologues in the concatenated vector. This reinforces the view that HR is the major pathway by which transforming DNA is integrated in Physcomitrella.

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“…Plants were subcultured in 7 dintervals. For protoplast isolation, protonema was grown in semi-continuous bioreactor cultures under a controlled pH of 4.5 (Hohe et al, 2002a). For knockout analysis, the moss plants were cultivated under different light conditions including white light, which was provided continuously by four Osram L18W/25 and three Lfluora L18W/77 fluorescent tubes; light flux of 38 mmol s À1 m À2 , red light with 658 nm, energy fluence rate of 6.5 W m À2 and far-red light with 730 nm, and energy fluence rate of 3.5 W m À2 (Sch.…”

Section: Methodsmentioning

confidence: 99%