Tidy dataset of the experimental design of the optimization of the alkali degumming process of Bombyx mori silk

Bucciarelli, Alessio; Greco, Gabriele H.; Corridori, Ilaria; Motta, Antonella; Pugno, Nicola

doi:10.1016/j.dib.2021.107294

Cited by 12 publications

(9 citation statements)

References 14 publications

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“…Second, since silk is a viscoelastic material 33 , forced silking procedures and spinning conditions affect the silk tensional states, the secondary structure content of the fibres, and thus their mechanical properties 34 , 35 . The same is true for artificial silk fibres as well as silk fibroin fibres, where the spinning protocols or the degumming process affect the mechanical properties of the fibres 23 , 36 , 37 . Third, a major cause for the variability may be found in the fact that the calculation of the stress is dependent on a precise measurement of the fibre cross-sectional area.…”

Section: Introductionmentioning

confidence: 90%

Artificial and natural silk materials have high mechanical property variability regardless of sample size

Greco

Mirbaha

Schmuck

et al. 2022

Sci Rep

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Silk fibres attract great interest in materials science for their biological and mechanical properties. Hitherto, the mechanical properties of the silk fibres have been explored mainly by tensile tests, which provide information on their strength, Young’s modulus, strain at break and toughness modulus. Several hypotheses have been based on these data, but the intrinsic and often overlooked variability of natural and artificial silk fibres makes it challenging to identify trends and correlations. In this work, we determined the mechanical properties of Bombyx mori cocoon and degummed silk, native spider silk, and artificial spider silk, and compared them with classical commercial carbon fibres using large sample sizes (from 10 to 100 fibres, in total 200 specimens per fibre type). The results confirm a substantial variability of the mechanical properties of silk fibres compared to commercial carbon fibres, as the relative standard deviation for strength and strain at break is 10–50%. Moreover, the variability does not decrease significantly when the number of tested fibres is increased, which was surprising considering the low variability frequently reported for silk fibres in the literature. Based on this, we prove that tensile testing of 10 fibres per type is representative of a silk fibre population. Finally, we show that the ideal shape of the stress–strain curve for spider silk, characterized by a pronounced exponential stiffening regime, occurs in only 25% of all tested spider silk fibres.

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Section: Introductionmentioning

confidence: 90%

Artificial and natural silk materials have high mechanical property variability regardless of sample size

Greco

Mirbaha

Schmuck

et al. 2022

Sci Rep

Self Cite

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“…The degumming process was done following a procedure previously described [41,42] and shown in Figure 1A. Briefly, delaminated silk cocoons were placed in a bath of Na2CO3 aqueous solution, with a ratio 1.1 g of salt every liter of deionized water, at 98 °C for 90 minutes.…”

Section: Regenerated Silk Fibroin Preparationmentioning

confidence: 99%

“…Finally, the material was wrapped in a double layer of paper and dried under the hood for at least 48 hours. To ensure the complete sericin removal the weight loss was checked to be between 25 % and 30 % as established by previous works [41,42].…”

Section: Regenerated Silk Fibroin Preparationmentioning

confidence: 99%

Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition Of Citric Acid

Bucciarelli¹,

Vighi²,

Bossi³

et al. 2023

Preprint

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Thermoplastic molded regenerated silk was proposed as structural material in tissue engineering applications, mainly for application in bone. The protocol allows to obtain a compact non-porous material with a compression modulus in the order of a Giga Pascal starting from silk fibroin by compressing a silk fibroin lyophilized sponge of powder in mold at temperature higher than the glass transition temperature (Tg). The main purpose of the produced resin was the osteofixation and other structural applications in which the lack of porosity was not an issue. In this work, we introduced the use of citric acid in the thermoplastic molding protocol of SF to obtain a porosity inside the structural material. In addition to the previously developed protocols the addition of citric acid allowed us to obtain a structural material. The CA powder during the compression acted as a template for the pore formation. In addition, the CA was able to effectively crosslink the SF chain improving the mechanical strength. This effect was proved both evaluating the compression modulus and by studying the spectra obtained by Fourier infrared spectroscopy (FTIR). This protocol may be applied in the near future to the production of a structural bone scaffold.

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“…SF is the internal protein of silk fiber and is extracted from it in form of an aqueous solution (regenerated Silk Fibroin, rSF) by chemical processing [13,14]. SF have an excellent mechanical strength [15], is biocompatible [16,17], bioresorbable [18,19], and in forms of gels and films it is transparent [20][21][22].…”

Section: Of 21mentioning

confidence: 99%

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

Bucciarelli¹

2022

Preprint

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Methacrylated silk (Sil-MA) is a chemically modified silk fibroin specifically designed to be crosslinkable under UV light. This allows the structuring of this material throught additive manufacturing techniques and then to easily prototype patient specific construct. In this study we used Sil-MA to produce single layer crosslinked structures that can be withdrawal and ejected recovering their shape after rehydration. A complete chemical and physical characterization of the material has been conducted. Additionally, we tested the material biocompatibility according to the International Standard Organization protocols (ISO 10993) ensuring the possibility to use it in future trials. The material was also tested to verify its ability to support the osteogenesis. Two different additive manufacturing techniques have been tested (a Digital Light Processing (DLP) UV projector and a pneumatic extrusion technique) to develop Sil-MA grid. Finally, we provide a proof-of-concept that the printed Sil-MA structures are injectable.

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Tidy dataset of the experimental design of the optimization of the alkali degumming process of Bombyx mori silk

Cited by 12 publications

References 14 publications

Artificial and natural silk materials have high mechanical property variability regardless of sample size

Artificial and natural silk materials have high mechanical property variability regardless of sample size

Porous Thermoplastic Molded Regenerated Silk Crosslinked by the Addition Of Citric Acid

Methacrylated Silk Fibroin Additive Manufacturing of Shape Memory Constructs with Possible Application in Bone Regeneration

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