Tickborne Arbovirus Surveillance in Market Livestock, Nairobi, Kenya

Sang, Rosemary; Onyango, Clayton; Gachoya, J.; Mabinda, Ernest; Konongoi, Samson L.; Ofula, Victor; Dunster, L. M.; Okoth, Fred; Coldren, Rodney L.; Tesh, Robert B.; Rosa, Amélia Travassos da; Finkbeiner, Stacy R.; Wang, David; Crabtree, Mary B.; Miller, Barry R.

doi:10.3201/eid1207.060253

Cited by 69 publications

(63 citation statements)

References 24 publications

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“…in Cameroon and Cote d'Ivoire and a human in the Central African Republic, and Barur virus (BARV), which has been isolated from ticks and a rodent (Rattus rattus) in India, ticks, fleas and mosquitoes of several species in Kenya and ticks in Somalia. [9][10][11][12][13][14][15] Phylogenetic analysis of partial N (nucleoprotein) protein sequences indicates that KCV is also related to several other viruses that have been isolated from bats in Africa and Asia. 16,17 These include Mount Elgon bat virus (MEBV) isolated in 1964 from a horseshoe bat (Rhinolophus hildebrandtii) in Kenya, Oita virus (OITAV) isolated in 1972 also from a horseshoe bat (Rhinolophus cornutus) in Japan, Kolente virus (KOLEV) isolated in 1985 from a roundleaf bat (Hipposideros jonesi) and a pool of ticks (Amblyomma (Theileriella) variegatum) in Guinea, and Fikirini virus (FKRV) isolated in 2011 from a roundleaf bat (Hipposideros vittatus) in Kenya.…”

Section: Introductionmentioning

confidence: 99%

Ledantevirus: A Proposed New Genus in the Rhabdoviridae has a Strong Ecological Association with Bats

Blasdell¹,

Guzmán²,

Widen³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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The Le Dantec serogroup of rhabdoviruses comprises Le Dantec virus from a human with encephalitis and Keuriliba virus from rodents, each isolated in Senegal. The Kern Canyon serogroup comprises a loosely connected set of rhabdoviruses many of which have been isolated from bats, including Kern Canyon virus from California, Nkolbisson virus from Cameroon, Central African Republic, and Cote d'Ivoire, Kolente virus from Guinea, Mount Elgon bat and Fikirini viruses from Kenya, and Oita virus from Japan. Fukuoka virus isolated from mosquitoes, midges, and cattle in Japan, Barur virus from a rodent in India and Nishimuro virus from pigs in Japan have also been linked genetically or serologically to this group. Here, we analyze the genome sequences and phylogenetic relationships of this set of viruses. We show that they form three subgroups within a monophyletic group, which we propose should constitute the new genus Ledantevirus.

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Section: Introductionmentioning

confidence: 99%

Ledantevirus: A Proposed New Genus in the Rhabdoviridae has a Strong Ecological Association with Bats

Blasdell¹,

Guzmán²,

Widen³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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“…DUGV has been reportedly isolated from several tick species, including A. gemma and R. pulchellus, the species from which Kupe virus was isolated (19,23,24). In the 1999 Kenya abattoir survey, DUGV was isolated from 4 species of ticks, A. variegatum, A. gemma, A. lepidum, and R. pulchellus (3). Although ≈1,000 specimens each of A. variegatum and A. lepidum were collected and tested in that study, no isolates of Kupe virus were found in those species, which suggested that vector hosts for DUGV and Kupe virus may differ (3).…”

Section: Discussionmentioning

confidence: 99%

“…Isolates of viruses were obtained from pools of ticks collected at abattoirs in Nairobi, Kenya, as described (3). The K611 isolate used in this study was obtained from a pool of Amblyomma gemma ticks in October 1999.…”

Section: Methodsmentioning

confidence: 99%

“…Full-length sequence was obtained by using a previously described method of primer walking and the 5′/3′ Rapid Amplification of cDNA Ends (RACE) Kit (Roche), which was used to determine the sequence of the segment ends (5). Fragments of the S (nt 413-916), M (nt 408-2372), and L (nt 6656-8185) segments from other Kupe virus isolates were also sequenced for comparison (3). Additionally, fragments of the S, M, and L segments from isolates of DUGV collected in 1999 from the Nairobi abattoirs were sequenced by using primers designed from the published sequence of DUGV (3).…”

Section: Nucleic Acid Sequencingmentioning

confidence: 99%

“…In a recent survey of ticks infesting market livestock in Nairobi, Kenya, we identifi ed 26 isolates of DUGV and additionally obtained several isolates of a virus that was identifi ed as a nairovirus related most closely to DUGV (3). We report further characterization of the K611 isolate of this virus, including the full-length genome.…”

mentioning

confidence: 99%

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Kupe Virus, a New Virus in the FamilyBunyaviridae, GenusNairovirus, Kenya

Crabtree¹,

Sang²,

Miller³

2009

Emerg. Infect. Dis.

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We have previously described isolation and preliminary identifi cation of a virus related to Dugbe virus (DUGV), family Bunyaviridae, genus Nairovirus. Six isolates of the virus were obtained from pools of Amblyomma gemma and Rhipicephalus pulchellus ticks collected from hides of cattle in Nairobi, Kenya, in October 1999. We report results of further characterization of this virus, including growth kinetics in cell culture and full-length genome sequencing and genetic characterization, which show it to be distinct from DUGV. We suggest that this is a new virus in the family Bunyaviridae, genus Nairovirus, and we propose that it be designated Kupe virus.

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