Tick-borne encephalitis virus strains of Western Siberia

Бахвалова, В. Н.; Рар, В. А.; Tkachev, Sergey; Матвеев, В. Н.; Matveev, Lev A.; Karavanov, A.S; Dobrotvorsky, Andrey K.; Морозова, О. В.

doi:10.1016/s0168-1702(00)00174-x

Cited by 36 publications

(24 citation statements)

References 14 publications

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“…Total nucleic acids were extracted from ticks with an IsoQuick nucleic acid extraction kit (ORCA Research, Bothell, Wash.), which was employed according to the manufacturer's instructions, followed by phenol-chloroform extraction and isopropanol precipitation (22). The presence of each pathogen was determined by using PCR with primer sets that have previously been used to detect the pathogens concerned and that are specific for genomic regions known to be present in all the isolates (3,21,24,25). The primers to detect the bmp genes were used to confirm the results of previous studies indicating that these genes are present in all B. burgdorferi sensu lato strains and appear in the following order: bmpD, bmpC, bmpA, and bmpB (12).…”

mentioning

confidence: 99%

PCR Detection of Borrelia burgdorferi Sensu Lato, Tick-Borne Encephalitis Virus, and the Human Granulocytic Ehrlichiosis Agent in Ixodes persulcatus Ticks from Western Siberia, Russia

et al. 2002

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PCR assays were used to test adult Ixodes persulcatus ticks from Western Siberia, Russia, for Borrelia burgdorferi sensu lato, tick-borne encephalitis virus (TBEV), and the human granulocytic ehrlichiosis (HGE) agent. Of the 150 ticks that were studied, 38% were infected with B. burgdorferi, 46% were infected with TBEV, and 8% were infected with the HGE agent. These three pathogens were distributed in the ticks independently of one another.

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confidence: 99%

PCR Detection of Borrelia burgdorferi Sensu Lato, Tick-Borne Encephalitis Virus, and the Human Granulocytic Ehrlichiosis Agent in Ixodes persulcatus Ticks from Western Siberia, Russia

et al. 2002

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“…TBE occurs widely across Europe, Russia, and Far-Eastern Asia, including Japan (Blaskovic et al, 1967; Korenberg and Kovalevskii, 1999; Lindgren and Gustafson, 2001;Ormaasen et al, 2001;Roggendorf et al, 1981), and more than 10,000 cases of the disease are reported annually. TBEV can be divided into three subtypes: 1) the Far-Eastern subtype, known as Russian spring summer encephalitis (RSSE) virus; 2) the European subtype, known as Central European encephalitis (CEE) virus; and 3) the Siberian subtype (Bakhvalova et al, 2000;Ecker et al, 1999). TBEV is transmitted by tick bites and is maintained in the zoonotic transmission cycle between ticks and wild vertebrate hosts, with humans acting as accidental hosts.…”

Section: Textmentioning

confidence: 99%

Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus

Yoshii

Ikawa

Chiba

et al. 2009

Journal of Virological Methods

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SummaryPreviously, a system for packaging tick-borne encephalitis virus (TBEV) subgenomic replicon RNAs into single-round infectious virus-like particles (VLPs) was developed. In the present study, VLPs were applied to measuring the levels of neutralizing antibodies against TBEV as an alternative to performing neutralization tests with live virus. As markers of VLP infection, the genes for GFP and luciferase were inserted into the TBEV replicon, which was then packaged into VLPs. The reporter genes were expressed in cells that were infected with the VLPs, and this infection was inhibited by neutralizing antibodies to TBEV. Serum samples from wild rodents were used to evaluate the neutralization test using VLPs. All the sera that were positive in the conventional neutralization test were also found to be positive in the neutralization test using VLPs, and there were highly significant correlations between the neutralization titres obtained using the native virus and those using VLPs.These results indicate that VLPs that express reporter genes represent a useful and safe alternative to conventional neutralization testing using live virus.Keywords: tick-borne encephalitis, virus-like particles, and neutralization test 3 TextTick-borne encephalitis virus (TBEV), which belongs to the family Flaviviridae genus Flavivirus, causes fatal encephalitis in humans with serious sequelae (Dumpis et al., 1999). TBE occurs widely across Europe, Russia, and Far-Eastern Asia, including Japan (Blaskovic et al., 1967; Korenberg and Kovalevskii, 1999; Lindgren and Gustafson, 2001;Ormaasen et al., 2001;Roggendorf et al., 1981), and more than 10,000 cases of the disease are reported annually. TBEV can be divided into three subtypes: 1) the Far-Eastern subtype, known as Russian spring summer encephalitis (RSSE) virus; 2) the European subtype, known as Central European encephalitis (CEE) virus; and 3) the Siberian subtype (Bakhvalova et al., 2000;Ecker et al., 1999). TBEV is transmitted by tick bites and is maintained in the zoonotic transmission cycle between ticks and wild vertebrate hosts, with humans acting as accidental hosts. The major tick vector of the European subtype is Ixodes ricinus, whereas I. persulcatus is the major tick vector for the other two viral subtypes (Ecker et al., 1999;Gaunt et al., 2001;Hayasaka et al., 2001;Lundkvist et al., 2001).Effective diagnosis of TBE infection relies on the detection of specific antibodies. However, the presence of cross-reactive antigenic structures among flaviviruses makes it difficult to differentiate between TBEV and other flavivirus infections/vaccinations using IgG-ELISA and the HI test (Holzmann et al., 1996). For cases that involve contact with other flaviviruses or vaccinations, a neutralizing test, which is the most specific serological test, is required to confirm the diagnosis of TBE infection. However, since TBEV is classified as a biosafety level 3 or 4 virus, a high-level biocontainment facility is required to handle the live virus required for neutralization testing. V...

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“…Among those proteins, NS1 is one of the most conserved flaviviral proteins, with 37-44% homology among different flaviviruses [4,5]. The molecular weight of NS1 protein is about 42 kDa, but in the JEV-infected cells the size of NS1 is approximately 48 kDa.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Japanese Encephalitis Virus NS1 Protein Expression in Cell by Small Interfering RNAs

et al. 2006

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Japanese encephalitis virus (JEV), a serious mosquitoborne flavivirus, causes an acute infection of the central system resulting in encephalitis of humans and many kinds of animals. A high proportion of the survivors exhibit neurogical and psychiatric sequelae. NS1 is one of important non-structural proteins, which was found to be associated with viral RNA replication. To inhibit NS1 expression, four small interfering RNAs (siRNAs) expression plasmids (pS-NS1A, pS-NS1B, pS-NS1C and pS-NS1D) were generated to target four different coding regions of the NS1 gene, and were separately co-transfected into Vero cells with an NS1-EGFP fusion expression plasmid pNS1-EGFP. NS1 expression was evaluated by fluorescence microscope, flow cytometry assay, Western blot and RT-PCR. The results revealed that pS-NS1B, pS-NS1C and pS-NS1D could effectively and specifically inhibit NS1 expression in Vero cells. Our data suggested that these siRNAs could be used to inhibit JEV replication by silencing NS1 protein expression in further study.

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Tick-borne encephalitis virus strains of Western Siberia

Cited by 36 publications

References 14 publications

PCR Detection of Borrelia burgdorferi Sensu Lato, Tick-Borne Encephalitis Virus, and the Human Granulocytic Ehrlichiosis Agent in Ixodes persulcatus Ticks from Western Siberia, Russia

PCR Detection of Borrelia burgdorferi Sensu Lato, Tick-Borne Encephalitis Virus, and the Human Granulocytic Ehrlichiosis Agent in Ixodes persulcatus Ticks from Western Siberia, Russia

Establishment of a neutralization test involving reporter gene-expressing virus-like particles of tick-borne encephalitis virus

Inhibition of Japanese Encephalitis Virus NS1 Protein Expression in Cell by Small Interfering RNAs

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