Tiaoshen Tongluo Attenuates Fibrosis by Modulating the TGF-β1/Smad Pathway in Endometrial Stromal Cells and a Rat Model of Intrauterine Adhesion

Niu, Hongping; Miao, Xiangshui; Zhan, Xingxiu; Zhou, Xiaona; Li, Xingyan; Jiang, Lijuan

doi:10.1155/2021/6675329

Cited by 9 publications

(8 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Feng et al also proved that Bushen Huoxue decoction was effective in the treatment of unexplained recurrent spontaneous abortion via activation of the PI3K/AKT pathway [ 16 ]. The traditional Chinese drug Tiaoshen Tongluo, which contained ingredients associated with the alleviation of fibrosis, was confirmed to attenuate endometrial fibrosis in a rat with IUA through TGF- β 1/Smad Pathway [ 22 ]. Similarly to Bushen Huoxue decoction, Guishen Huoxue decoction is a traditional drug formulation, consisting of Paeonia lactiflora , Ligusticum wallichii , the root of red-rooted salvia, Angelica sinensis , fruit of Chinese wolfberry, Astragalus membranaceus , Spatholobus stem, fruit of glossy privet, Chinese yam, fruit of medicinal cornel, prepared rehmannia root, seed of Chinese seed of Chinese dodder, improves renal function and promotes blood circulation [ 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Potential Molecular Mechanism of Guishen Huoxue Decoction against Intrauterine Adhesion Based on Network Pharmacology

Zhang

Yuan

Huang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Objective. Intrauterine adhesion (IUA) represents an endometrial repair disorder that is associated with menstrual disorders, recurrent pregnancy loss, and infertility. This study aimed to explore the underlying biological mechanisms of Guishen Huoxue decoction for the treatment of IUA based on network pharmacology. Methods. The selection of active compounds for Guishen Huoxue decoction and prediction of relevant targets were performed by the TCMSP and Swiss Target Prediction databases, respectively. The targets of IUA were obtained by three databases, including Online Mendelian Inheritance in Man (OMIM), DisGeNET, and GeneCards. The drug-disease regulatory network was constructed via Cytoscape software, following the acquisition of common genes of active compounds of drug Guishen Huoxue decoction and disease IUA, which was carried out through Venny software. Protein-protein interaction (PPI) network and function enrichment analyses were performed. Results. According to the data obtained from TCMSP, a total of 200 potential active compounds of Guishen Huoxue decoction and their related targets (1068) were screened by the Swiss Target Prediction database. 1303 disease targets and 134 common targets were identified. The drug-disease regulatory network showed that 165 active compounds were found to be involved in the treatment of IUA. Among 134 common targets, AKT1, SRC, TP53, VEGFA, and IL-6 were predicted as core genes against IUA. PI3K-Akt, Rap1, Ras, and AGE-RAGE were the main signaling pathways that participated in the treatment of Guishen Huoxue decoction for IUA. Conclusion. The active compounds of Guishen Huoxue decoction confer therapeutic effects against IUA by regulating fibrosis, inflammation, and oxidative stress through major signaling pathways such as PI3K-Akt and AGE-RAGE.

show abstract

Section: Discussionmentioning

confidence: 99%

Potential Molecular Mechanism of Guishen Huoxue Decoction against Intrauterine Adhesion Based on Network Pharmacology

Zhang

Yuan

Huang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…According to a report by Niu et al, the Tiaoshen Tongluo recipe, a traditional Chinese medicine herbal formula, can upregulate Smad7 and downregulate TGF-β1 to alleviate fibrotic tissue formation in an IUA rat model [ 88 ]. Transplantation of decellularized and lyophilized amniotic membranes ameliorated the degree of fibrosis by suppressing TGF-β1 expression [ 89 ].…”

Section: Discussionmentioning

confidence: 99%

Extracorporeal Shock Wave Therapy Combined with Platelet-Rich Plasma during Preventive and Therapeutic Stages of Intrauterine Adhesion in a Rat Model

et al. 2022

View full text Add to dashboard Cite

Intrauterine adhesion (IUA) is caused by artificial endometrial damage during intrauterine cavity surgery. The typical phenotype involves loss of spontaneous endometrium recovery and angiogenesis. Undesirable symptoms include abnormal menstruation and infertility; therefore, prevention and early treatment of IUA remain crucial issues. Extracorporeal shockwave therapy (ESWT) major proposed therapeutic mechanisms include neovascularization, tissue regeneration, and fibrosis. We examined the effects of ESWT and/or platelet-rich plasma (PRP) during preventive and therapeutic stages of IUA by inducing intrauterine mechanical injury in rats. PRP alone, or combined with ESWT, were detected an increased number of endometrial glands, elevated vascular endothelial growth factor protein expression (hematoxylin-eosin staining and immunohistochemistry), and reduced fibrosis rate (Masson trichrome staining). mRNA expression levels of nuclear factor-kappa B, tumor necrosis factor-α, transforming growth factor-β, interleukin (IL)-6, collagen type I alpha 1, and fibronectin were reduced during two stages. However, PRP alone, or ESWT combined with PRP transplantation, not only increased the mRNA levels of vascular endothelial growth factor (VEGF) and progesterone receptor (PR) during the preventive stage but also increased PR, insulin-like growth factor 1 (IGF-1), and IL-4 during the therapeutic stage. These findings revealed that these two treatments inhibited endometrial fibrosis and inflammatory markers, thereby inhibiting the occurrence and development of intrauterine adhesions.

show abstract

“…For example, microRNA‐29b hindered endometrial fibrosis and inhibited EMT by reducing TGF‐β and p‐SMAD3 expression in a rat model of intrauterine adhesion 47 . TGF‐β, p‐SMAD2, and p‐SMAD3 levels were notably increased in the endometrial tissues of rats with intrauterine adhesion relative to the sham group 48,49 . Moreover, endometrial tissues of rats with intrauterine adhesion had serious endometrial tissue damage and reduced vimentin expression compared to the sham group 48 .…”

Section: Discussionmentioning

confidence: 99%

“…TGF‐β, p‐SMAD2, and p‐SMAD3 levels were notably increased in the endometrial tissues of rats with intrauterine adhesion relative to the sham group 48,49 . Moreover, endometrial tissues of rats with intrauterine adhesion had serious endometrial tissue damage and reduced vimentin expression compared to the sham group 48 . Hence, the effect of SNHG6 ORF#2‐encoded peptide on the TGF‐β/SMAD pathway was examined in endometrial cells.…”

Section: Discussionmentioning

confidence: 99%

A short peptide encoded by long non‐coding RNA small nucleolar RNA host gene 6 promotes cell migration and epithelial–mesenchymal transition by activating transforming growth factor‐beta/SMAD signaling pathway in human endometrial cells

Zhou¹,

Du²,

Zhou³

et al. 2022

J of Obstet and Gynaecol

View full text Add to dashboard Cite

Background Endometrial dysfunction is closely correlated with the development of multiple severe gynecological disorders including intrauterine adhesion. Accumulating evidence supports that some long non‐coding RNAs (lncRNAs) have peptide‐coding potential. In this text, the peptide‐coding ability of lncRNA SNHG6 was examined. Also, the effects of an SNHG6‐encoded peptide on the viability and migration of human endometrial stromal cells (hESCs) and human endometrial epithelial cells (hEECs) and related molecular mechanisms were explored. Methods The peptide‐encoding potential of SNHG6 was predicted by FuncPEP and getorf databases and validated by western blot assay. Cell viability was tested by cell counting kit‐8 assay. Cell migratory ability was examined by wound healing and transwell migration assays. Protein levels of genes were measured by western blot assay. Results Prediction analysis suggested that SNHG6 had the potential peptide‐coding ability and multiple open‐reading frames (ORFs). Western blot validated that SNHG6 ORF#1 and ORF#2 could translate into short peptides. SNHG6 ORF#2 overexpression facilitated cell migration and epithelial–mesenchymal transition (EMT) in hESCs and hEECs, while these effects were abrogated by transforming growth factor‐beta (TGF‐β)/SMAD signaling inhibitor GW788388. Moreover, GW788388 inhibited the increase of p‐SMAD2 and p‐SMAD3 levels induced by SNHG6 ORF#2 in hESCs. SNHG6 ORF#2‐encoded peptide did not influence endometrial stromal and epithelial cell viability. Conclusions LncRNA SNHG6 ORF#1 and ORF#2 could translate into small peptides and SNHG6 ORF#2 overexpression promoted cell migration and EMT by activating the TGF‐β/SMAD pathway in hESCs and hEECs, suggesting the potential roles of SNHG6‐encoded peptides in the development of endometrial stromal and epithelial cells and related gynecological diseases.

show abstract

Tiaoshen Tongluo Attenuates Fibrosis by Modulating the TGF-β1/Smad Pathway in Endometrial Stromal Cells and a Rat Model of Intrauterine Adhesion

Cited by 9 publications

References 44 publications

Potential Molecular Mechanism of Guishen Huoxue Decoction against Intrauterine Adhesion Based on Network Pharmacology

Potential Molecular Mechanism of Guishen Huoxue Decoction against Intrauterine Adhesion Based on Network Pharmacology

Extracorporeal Shock Wave Therapy Combined with Platelet-Rich Plasma during Preventive and Therapeutic Stages of Intrauterine Adhesion in a Rat Model

A short peptide encoded by long non‐coding RNA small nucleolar RNA host gene 6 promotes cell migration and epithelial–mesenchymal transition by activating transforming growth factor‐beta/SMAD signaling pathway in human endometrial cells

Contact Info

Product

Resources

About