Tianhuang formula regulates adipocyte mitochondrial function by AMPK/MICU1 pathway in STZ-induced T2DM mice

Abstract

Background: Tianhuang formula (THF) is a Chinese medicine prescription that is patented and clinically approved, has been shown to improve energy metabolism, but the underlying mechanism remains poorly understood. The purpose of this study is to clarify the potential mechanisms of THF in the treatment of type 2 diabetes mellitus (T2DM). Methods: A murine model of T2DM was induced by high-fat diet (HFD) feeding combined with low-dose streptozocin (STZ) injections, and the diabetic mice were treated with THF by gavaging for consecutive 10 weeks. Fasting blood glucose (FBG), serum insulin, blood lipid, mitochondrial Ca2+ (mCa2+) levels, mitochondrial membrane potential (MMP) as well as ATP production were analyzed. The target genes and proteins expression of visceral adipose tissue (VAT) was tested by RT-PCR and western blot, respectively. The underlying mechanism of the regulating energy metabolism effect of THF was further explored in insulin resistance model of 3T3-L1 adipocytes cultured with dexamethasone (DXM). Results: THF restored impaired glucose tolerance and insulin resistance in diabetic mice. Serum levels of lipids were significantly decreased, as well as fasting blood glucose and insulin in THF-treated mice. THF regulated mCa2+ uptake, increased MMP and ATP content in VAT. THF increased the mRNA and protein expression of AMPK, phosphorylated AMPK (p-AMPK), MICU1, sirtuin1 (SIRT1) and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α). THF could increase the mCa2+ level of 3T3-L1 adipocytes and regulate mitochondrial function. The protein expression of AMPK, p-AMPK, mCa2+ uniporter (MCU) and MICU1 decreased upon adding AMPK inhibitor compound C to 3T3-L1 adipocytes and the protein expression of MCU and MICU1 decreased upon adding the MCU inhibitor ruthenium red. Conclusions: These results demonstrated that THF ameliorated glucose and lipid metabolism disorders in T2DM mice through improvement of AMPK/MICU1 pathway-dependent mitochondrial function in adipose tissue.