TiAl6V4 particles promote osteoclast formation via autophagy-mediated downregulation of interferon-beta in osteocytes

Wang, Zhenheng; Deng, Zhong‐Liang; Gan, Jingjing; Zhou, Gang; Shi, Tongguo; Wang, Zhenzhen; Huang, Zhen; Hu, Qian; Bao, Nirong; Guo, Tao; Chen, Jiangning; Zhang, Junfeng; Liu, Feng; Dong, Lei; Zhao, Jianning

doi:10.1016/j.actbio.2016.11.020

Cited by 41 publications

(37 citation statements)

References 54 publications

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“…The wear particle-induced calvarial osteolysis model in 8-week-old C57BL/J6 mice has been described previously. 17,18 Briefly, the mice were anesthetized, and the cranial periosteum was separated from the calvarium by sharp dissection. Forty microliters (40 mg/mL) of the CoPs suspension was embedded under the periosteum around the middle suture of the calvaria.…”

Section: Particle Preparationmentioning

confidence: 99%

Probiotics protect mice from CoCrMo particles-induced osteolysis

Wang¹,

Xue²,

Bai³

et al. 2017

IJN

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Wear particle-induced inflammatory osteolysis is the primary cause of aseptic loosening, which is the most common reason for total hip arthroplasty (THA) failure in the med- and long term. Recent studies have suggested an important role of gut microbiota (GM) in modulating the host metabolism and immune system, leading to alterations in bone mass. Probiotic bacteria administered in adequate amounts can alter the composition of GM and confer health benefits to the host. Given the inflammatory osteolysis that occurs in wear debris-induced prosthesis loosening, we examined whether the probiotic Lactobacillus casei could reduce osteolysis in a mouse calvarial resorption model. In this study, L. casei markedly protected mice from CoCrMo particles (CoPs)-induced osteolysis. Osteoclast gene markers and the number of osteoclasts were significantly decreased in L. casei -treated mice. Probiotic treatment decreased the M1-like macrophage phenotype indicated by downregulation of tumor necrosis factor α (TNF-α), interleukin (IL)-6 and inducible nitric oxide synthase (iNOS) and increased the M2-like macrophage phenotype indicated by upregulation of IL-4, IL-10 and arginase. Collectively, these results indicated that the L. casei treatment modulated the immune status and suppressed wear particle-induced osteolysis in vivo. Thus, probiotic treatment may represent a potential preventive and therapeutic approach to reduced wear debris-induced osteolysis.

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Section: Particle Preparationmentioning

confidence: 99%

Probiotics protect mice from CoCrMo particles-induced osteolysis

Wang¹,

Xue²,

Bai³

et al. 2017

IJN

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“…The TiAl6V4 particles (TiPs) used in the present study were produced and described as previously described . The particles were washed with absolute alcohol for five times followed by five washes with sterile water, before autoclaved for 15 minutes at 121°C and 15 psi for sterilization.…”

Section: Methodsmentioning

confidence: 99%

“…The TiAl6V4 particles (TiPs) used in the present study were produced and described as previously described. 16,17 The particles were washed with absolute alcohol for five times followed by five washes with sterile water, before autoclaved for 15 minutes at 121°C and 15 psi for sterilization. A quantitative Limulus Amebocyte Lysate (LAL) Assay (Charles River, Grand Island, UK) was used to determine endotoxin level in the particles, and only negative testing for endotoxin particles at a detection level of <0.25 EU/mL were suspended in sterile phosphate-buffered saline (PBS) and used in the study.…”

Section: Particle Preparationmentioning

confidence: 99%

“…Sirtuin 1 (SIRT1), a highly conserved nicotinamide adenine dinucleotide (NAD)‐dependent histone deacetylase, has recently been reported to be involved in aging, inflammation and cell cycle . Studies have suggested that H 2 S may regulate the expression and activity of SIRT1.…”

mentioning

confidence: 99%

“…15 Sirtuin 1 (SIRT1), a highly conserved nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase, has recently been reported to be involved in aging, inflammation and cell cycle. 16 Studies have suggested that H 2 S may regulate the expression and activity of SIRT1. Recently, Zhu et al reported that the expression of SIRT1 was decreased under oxidative stress, and H 2 S could elevate its level in dose and time dependent manner in H9c2 cardiomyocytes.…”

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confidence: 99%

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Hydrogen sulfide protects against particle‐induced inflammatory response and osteolysis via SIRT1 pathway in prosthesis loosening

et al. 2020

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Wear debris‐induced osteolysis and ensuing aseptic loosening is the main cause of implant failure and revision surgery. Wear debris‐induced inflammatory response plays key roles in peri‐implant osteolysis. Recently, substantial of evidence suggests that hydrogen sulfide (H2S), the third gasotransmitter, is a critical player regulating inflammation. However, the role and therapeutic potential of H2S in wear debris‐induced inflammation and osteolysis remains to be defined. In the present study, we investigated the effect of H2S on wear debris‐induced pro‐inflammatory cytokines expression and osteolysis in vitro and in vivo. With a slow‐releasing H2S donor GYY4137, our study demonstrated that H2S attenuated wear debris‐induced osteolysis and osteoclastogenesis in murine calvaria resorption models. The expression of tumor necrosis factor‐alpha (TNF‐α), interleukin‐1β (IL‐1β), and interleukin‐6 (IL‐6) that stimulated by wear particles were significantly reduced by GYY4137. Further, the level of sirtuin 1 (SIRT1), which possesses anti‐inflammation property, was examined in vivo and in macrophages. And we found that wear debris decreased the expression of SIRT1. Cotreated macrophages with GYY4137 in part reversed the decline of SIRT1. More importantly, with the SIRT1 recombinant lentivirus and small interfering RNAs (siRNA) against SIRT1, our data indicated that SIRT1 mediated the inhibitory effects of GYY4137 on wear debris‐induced inflammation. Collectively, these results suggested that exogenous H2S production (via H2S donors) may represent a potential approach for the treatment of wear particle‐induced osteolysis.

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Pro-inflammatory Cytokines and Osteocytes

Zhou

Pathak

2019

Curr Osteoporos Rep

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TiAl6V4 particles promote osteoclast formation via autophagy-mediated downregulation of interferon-beta in osteocytes

Cited by 41 publications

References 54 publications

Probiotics protect mice from CoCrMo particles-induced osteolysis

Probiotics protect mice from CoCrMo particles-induced osteolysis

Hydrogen sulfide protects against particle‐induced inflammatory response and osteolysis via SIRT1 pathway in prosthesis loosening

Pro-inflammatory Cytokines and Osteocytes

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