Thyroxin Analogs in Tadpoles

Kollros, Jerry J.; Frieden, Earl

doi:10.1126/science.134.3482.892

Cited by 4 publications

(1 citation statement)

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“…Metamorphosis is a significant transitional period in amphibian development, marked by drastic thyroid hormone-driven changes in physiology and gene expression across the body, including the central nervous system ( Kollros and Frieden 1961 ; Yaoita and Nakajima 2018 ). In the amphibians Microhyla fissipes ( Zhao et al 2016 ) and Ambystoma velasci ( Palacios-Martinez et al 2020 ), transcriptomic profiles over the metamorphic period have been collected and compared to illuminate how gene expression changes as metamorphosis proceeds.…”

Section: Introductionmentioning

confidence: 99%

Temporal and spatial transcriptomic dynamics across brain development in Xenopus laevis tadpoles

Huang

McKeown

et al. 2021

G3 Genes|Genomes|Genetics

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Amphibian metamorphosis is a transitional period that involves significant changes in the cell-type populations and biological processes occurring in the brain. Analysis of gene expression dynamics during this process may provide insight into the molecular events underlying these changes. We conducted differential gene expression analyses of the developing Xenopus laevis tadpole brain during this period in two ways: first, over stages of the development in the midbrain and, second, across regions of the brain at a single developmental stage. We found that genes pertaining to positive regulation of neural progenitor cell proliferation as well as known progenitor cell markers were upregulated in the midbrain prior to metamorphic climax; concurrently, expression of cell cycle timing regulators decreased across this period, supporting the notion that cell cycle lengthening contributes to a decrease in proliferation by the end of metamorphosis. We also found that at the start of metamorphosis, neural progenitor populations appeared to be similar across the fore-, mid-, and hindbrain regions. Genes pertaining to negative regulation of differentiation were upregulated in the spinal cord compared to the rest of the brain, however, suggesting that different programs may regulate neurogenesis there. Finally, we found that regulation of biological processes like cell fate commitment and synaptic signaling follow similar trajectories in the brain across early tadpole metamorphosis and mid- to late-embryonic mouse development. By comparing expression across both temporal and spatial conditions, we have been able to illuminate cell-type and biological pathway dynamics in the brain during metamorphosis.

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