Thyrotropin-Releasing Hormone Effects on Guinea Pig Antrum

Oouchi, Michiko; Ichikawa, Santa

doi:10.1016/s0016-5085(85)80070-6

Cited by 8 publications

(8 citation statements)

References 26 publications

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“…15 The GI response to TRH administered subcutaneously in the guinea pig has not been studied in detail; however, one in vitro study showed that TRH stimulates motility of the gastric antrum, small intestine and taenia coli of the guinea pig. 16 In our study, TRH increased upper GI transit and the number and weight of fecal pellet expulsion, although the effect did not reach statistical significance. In the altered guinea pig model induced by TRH, SKI3246 significantly decreased number and weight of fecal pellets at 300 mg/kg and 600 mg/kg doses; however, it did not affect upper GI transit in this model.…”

Section: Discussioncontrasting

confidence: 74%

The Effects of an Extract ofAtractylodes JaponicaRhizome, SKI3246 on Gastrointestinal Motility in Guinea Pigs

Park

Chon

Lee

et al. 2015

J Neurogastroenterol Motil

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Background/AimsThere are limited therapeutic options available for irritable bowel syndrome with diarrhea (IBS-D). We tested the effects of Atractylodes japonica rhizom e, a perennial plant native to North Asia, on both upper and lower gastrointestinal (GI) motility in guinea pigs. MethodsThe extract of A. japonica rhizome was administered orally at different doses to test its effects on upper GI motility as determined from charcoal transit in native guinea pigs and in guinea pigs pretreated with thyrotropin-releasing hormone or mustard oil. Regarding its effect on lower GI motility, the removed guinea pig colon was suspended in a chamber containing Krebs-Henseleit solution and the transit time of artificial feces was measured with various dilutions of the extract. As for in vivo assay, weight and number of fecal pellets expelled were determined under the same drug preparation used in upper GI motility experiment. ResultsThe extract of A. japonica rhizome had no significant effect on upper GI motility in either normal or altered physiological states. However, the extract increased colonic transit time in the in vitro model. In the fecal expulsion study, the cumulative weight and number of pellets did not differ significantly between the control group and groups treated with the extracts. In the animals pretreated in vivo with thyrotropin-releasing hormone, however, the weight and number of fecal pellets were significantly decreased in animals treated with 300 mg/kg and 600 mg/kg doses of extract. Conclusions

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Section: Discussioncontrasting

confidence: 74%

The Effects of an Extract ofAtractylodes JaponicaRhizome, SKI3246 on Gastrointestinal Motility in Guinea Pigs

Park

Chon

Lee

et al. 2015

J Neurogastroenterol Motil

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“…In vitro, several reports showed that the TRH-induced response of gastrointestinal motility was mediated by myenteric neurons, because the response to TRH was abolished by tetrodotoxin [7,9]. Oouchi and Ichikawa [7] concluded that TRH stimulated cholinergic and nonadrenergic, noncholinergic inhibitory neurons in the myenteric plexus of the guinea pig antrum to enhance the release of acetylcholine and some transmitter as yet un known.…”

Section: Discussionmentioning

confidence: 99%

“…Oouchi and Ichikawa [7] concluded that TRH stimulated cholinergic and nonadrenergic, noncholinergic inhibitory neurons in the myenteric plexus of the guinea pig antrum to enhance the release of acetylcholine and some transmitter as yet un known. Bruce et al [8] proposed that TRH stimulated rat gastric antral tissue through a noncholinergic excitatory mechanism, probably a histaminergic pathway.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro. TRH stimulates the motility of the gastric antrum in guinea pig [7] and rat [8], and of the duodenum and the taenia coli in guinea pig [9], On the other hand, Tonoue et al [10] reported that TRH has an inhibitory effect on rat duodenum. While the excitatory effects of TRH are apparently neurally mediated, the inhibitory effect seems to be exerted directly on the smooth muscle cells.…”

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confidence: 99%

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Direct Inhibitory Effect of Thyrotropin-Releasing Hormone on Isolated Cecal Circular Smooth Muscle Cells of Guinea Pig

et al. 1996

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Smooth muscle cells isolated from cecal circular smooth muscle of the guinea pig were used to determine whether thyrotropin-releasing hormone (TRH) can inhibit the contractile response produced by 10^-6M carbachol by exerting a direct action on muscle cells. In addition, the inhibitory effect of 2´,5´-dideoxy-adenosine (an inhibitor of adenylate cyclase), phorbol 12-myristate 13-acetate (an inhibitor of particulate guanylate cyclase), 6-anilinoquinoline-5,8-quinone (an inhibitor of soluble guanylate cyclase) and Nω-nitro-L-arginine methyl ester (an inhibitor of nitric oxide synthase) on the TRH-induced relaxation of cecal circular smooth muscle cells was examined. TRH inhibited the contractile response produced by 10^-6M carbachol in a concentration-dependent manner, with an IC50 value of 4 nM. 2´,5´-Dideoxyadenosine and phorbol 12-myristate 13-acetate did not have any significant effect on the TRH-induced relaxation. On the other hand, 6-anilinoquinoline-5,8-quinone and Nω-nitro-L-arginine methyl ester significantly inhibited the relaxation produced by TRH. Our findings show that TRH has a direct inhibitory effect on the isolated cecal circular smooth muscle cells via activation of nitric oxide synthase and soluble guanylate cyclase.

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“…It has also been found that the motor actions of TRH are inhibitory and/or stimulatory, depending on the species and the region of the digestive system under study. The rat duodenum longitudinal muscle is relaxed by TRH via an action directly on the muscle (10) while the effects of the peptide on gastrointestinal muscle preparations from the guinea pig are mediated by nerves (11,12).…”

Section: Introductionmentioning

confidence: 99%

Thyrotropin‐Releasing Hormone Potently Modulates Peristaltic Motor Activity of the Guinea Pig Small Intestine

Holzer

Pertl

Wolf

1989

Neurogastroenterology Motil

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Thyrotropin‐releasing hormone (TRH) is present in enteric neurons of the guinea pig intestine, which indicates it may have a role in the control of gut motility. Isolated segments of the guinea pig duodenum‐jejunum and ileum were used to examine the effect of TRH on intestinal peristalsis. The peristaltic waves in the proximal small intestine were triggered at a higher intraluminal pressure and exhibited a higher frequency and a lower amplitude than those in the ileum. TRH (0.3–300 nM) induced a concentration‐related increase in the pressure threshold at which peristaltic waves were elicited and a moderate decrease in the amplitude of the peristaltic waves in both regions. However, while the frequency of the peristaltic waves was increased by the peptide in the ileum, it was diminished by TRH (0.3 and 3 nM) in the duodenum‐jejunum. Since the contractile effect of carbachol on the circular muscle of the duodenum‐jejunum remained unaffected by TRH, it appears that the peptide does not act on the muscle directly but influences peristalsis by acting on enteric neurons. The data further suggest that TRH released from enteric neurons may play a physiologic role in the regulation of propulsive motor activity of the gut.

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Thyrotropin-Releasing Hormone Effects on Guinea Pig Antrum

Cited by 8 publications

References 26 publications

The Effects of an Extract ofAtractylodes JaponicaRhizome, SKI3246 on Gastrointestinal Motility in Guinea Pigs

The Effects of an Extract ofAtractylodes JaponicaRhizome, SKI3246 on Gastrointestinal Motility in Guinea Pigs

Direct Inhibitory Effect of Thyrotropin-Releasing Hormone on Isolated Cecal Circular Smooth Muscle Cells of Guinea Pig

Thyrotropin‐Releasing Hormone Potently Modulates Peristaltic Motor Activity of the Guinea Pig Small Intestine

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