Thyrotropin inhibits osteogenic differentiation of human periodontal ligament stem cells

Abstract

Background and Objective: Periodontal ligament stem cells (PDLSCs) are derived from the periodontal ligament and have the characteristics of pluripotent differentiation, including osteogenesis, and are one of the important seed cells in oral tissue engineering. Thyrotropin (TSH) has been shown to regulate bone metabolism independently of thyroid hormone, including the fate of osteoblasts and osteoclasts, but whether it affects osteogenic differentiation of PDLSCs is unknown. Materials and Methods:PDLSCs were isolated and cultured from human periodontal ligament and grown in osteogenic medium (containing sodium β-glycerophosphate, ascorbic acid, and dexamethasone). Recombinant human TSH was added to the culture medium. Osteogenic differentiation of PDLSCs was assessed after 14 days by staining with alkaline phosphatase and alizarin red and by detection of osteogenic differentiation genes. Differentially expressed genes (DEGs) in PDLSCs under TSH were detected by high-throughput sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyzed the biological functions and signaling pathways involved in DEGs. Results:We found that osteogenic differentiation of PDLSCs was significantly inhibited in the presence of TSH: including decreased calcium nodule formation, decreased alkaline phosphatase levels, and decreased collagen synthesis. Using high-throughput sequencing, we found changes in the expression of some osteogenesis-related genes, which may be the reason that TSH inhibits osteogenic differentiation of PDLSCs. Conclusion:Unless TSH is ≥10 mU/L, patients with subclinical hypothyroidism usually do not undergo thyroxine supplementation therapy. However, in this work, we found that elevated TSH inhibited the osteogenic differentiation of PDLSCs. Therefore,

The role of microRNAs in pathophysiology and diagnostics of metabolic complications in obstructive sleep apnea patients

“…Given the fact that OSA patients generally have worse progress in diabetic ulcer treatment, the protective effect of upregulated miRNA-31 on wound healing may work only in some cases or be a part of a specific subset responsible for the repair (Maltese et al, 2018). The same miRNA was upregulated in the retina (Kovacs et al, 2011) and periodontal ligament (Zhen et al, 2017) of diabetic rats, and in endothelial progenitor cells obtained from T2DM patients (Lian et al, 2018). (Freude et al, 2012).…”

Periodontitis and thyroid function: A bidirectional Mendelian randomization study

“…A scoping review also found a positive relationship between hypothyroidism and periodontitis 6 . In turn, there is evidence that thyroid dysfunction was significantly linked to the development of periodontitis, independent of various risk factors for periodontitis 7–9 . However, the evidence for causal relation concerning periodontitis and thyroid function is limited, with only few observational studies available.…”

Orally Derived Stem Cell-Based Therapy in Periodontal Regeneration: A Systematic Review and Meta-Analysis of Randomized Clinical Studies

“…The expression of specific surface antigens, including CD44, CD73, CD90, and CD105, helps characterize MSCs, while lacking hematopoietic and endothelial markers [30]. Indeed, MSCs are defined by their plastic adherence, capacity of self-renovation, and the potential for differentiation in vitro into different types of cells, like osteoblasts, adipocytes, and chondroblasts, under specific stimuli [31,32]. Many intraoral and dental sources of MSCs are available, for example, dental pulp, periodontal ligament, bone marrow from alveolar bone, dental follicle, gingival connective tissue, or apical papilla [33][34][35][36].…”