Thyroid Transcription Factor 1 Is Calcium Modulated and Coordinately Regulates Genes Involved in Calcium Homeostasis in C Cells

Suzuki, Koichi; Lavaroni, Stefano; Mori, Atsumi; Okajima, Fumikazu; Kimura, Shioko; Katoh, Ryohei; Kawaoi, Akira; Kohn, Leonard D.

doi:10.1128/mcb.18.12.7410

Cited by 74 publications

(72 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells were grown in Coon's modified F-12 medium (Sigma Chemical Co) containing 5% heat-treated, mycoplasma-free calf serum (GIBCO Laboratories Life Technologies), 1 mM nonessential amino acids (GIBCO), and supplemented with six hormones or growth factors (termed 6H) that included bovine TSH (1 10 10 M), insulin (10 µg/ml), cortisol (0·4 ng/ml), transferrin (5 µg/ml), glycyl--histidyl--lysine acetate (10 ng/ml), and somatostatin (10 ng/ml). Bovine Tg was added directly to the culture medium as described previously (Royaux et al 2000, Suzuki et al 1998c, 1999c.…”

Section: Cellsmentioning

confidence: 99%

“…Total RNA isolation and Northern blot analysis were performed as previously described (Suzuki et al 1998b(Suzuki et al , 1998c. RNA was prepared using an RNeasy Mini Kit (Qiagen).…”

Section: Rna Isolation and Northern Blot Analysismentioning

confidence: 99%

“…Rat thyroid tissues were fixed with neutral-buffered formalin and immunostained as previously described (Suzuki et al , 1992(Suzuki et al , 1998a(Suzuki et al , 1998c. Briefly, deparaffinized and rehydrated sections were treated with 3% H 2 O 2 for 10 min at room temperature, washed in PBS, incubated with 10% normal goat serum, and incubated with rabbit anti-rat Tg antibody (a gift from Dr A Kawaoi, Yamanashi Medical University, Yamanashi, Japan) diluted 1:1000.…”

Section: Immunohistochemistrymentioning

confidence: 99%

See 2 more Smart Citations

Differential regulation of apical and basal iodide transporters in the thyroid by thyroglobulin

Suzuki

Kohn²

2006

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

We have shown that thyroglobulin (Tg) is a potent autocrine regulator of thyroid-specific gene expression, and proposed that the accumulated follicular Tg within the colloid is a major factor in determining follicular function. In the present report, we examined the effect of Tg on the action of TSH/cAMP and iodine with special focus on the regulation of basolateral and apical iodide transporters; the sodium/iodide symporter (NIS) and the pendred syndrome gene (PDS) by Tg. We show that expression of NIS and PDS are differentially regulated by Tg concentration and exposure time. In addition, we found that PDS gene was induced by TSH/cAMP and iodide in the presence of Tg. Based on these results, we propose a model for the physiological turnover of follicular function that is dynamically regulated by Tg.

show abstract

Section: Cellsmentioning

confidence: 99%

“…Total RNA isolation and Northern blot analysis were performed as previously described (Suzuki et al 1998b(Suzuki et al , 1998c. RNA was prepared using an RNeasy Mini Kit (Qiagen).…”

Section: Rna Isolation and Northern Blot Analysismentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

See 1 more Smart Citation

Differential regulation of apical and basal iodide transporters in the thyroid by thyroglobulin

Suzuki

Kohn²

2006

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

show abstract

“…surfactant proteins and the Clara cell secretory protein) genes (2-7). TTF-1 has been reported to be expressed also in the adult rat parathyroid cells and several other adult tissues (including skin, esophagus, retina, anterior pituitary, cerebellum, and hippocampus) (8,9). Recently, it has been shown that postnatal hypothalamic TTF-1 expression is strongly and transiently up-regulated immediately before puberty in female rats and is associated with the neuroendocrine process of female sexual development (10).…”

mentioning

confidence: 99%

Nestin Is a Neuroepithelial Target Gene of Thyroid Transcription Factor-1, a Homeoprotein Required for Forebrain Organogenesis

Lonigro

Donnini

Zappia

et al. 2001

Journal of Biological Chemistry

View full text Add to dashboard Cite

Thyroid transcription factor-1 (TTF-1, also known as NKX2.1 and T/EBP), a transcription factor belonging to the NKX-2 family of homeodomain-containing genes, plays an essential role in the organogenesis of the thyroid gland, lung, and ventral forebrain. Nestin is an intermediate filament protein strongly expressed in multipotential neuroepithelial stem cells and rapidly down-regulated during postnatal life. Here we show that stable fibroblastic clones expressing TTF-1 acquire a phenotype reminiscent of neuroepithelial cells in culture and up-regulate the endogenous nestin gene. TTF-1 transactivates in HeLa and NIH3T3 cells a reporter gene driven by a central nervous system-specific enhancer element from the second intron of the rat nestin gene, where it recognizes a DNA-binding site (NestBS) whose sequence resembles a nuclear hormone/cAMP-responsive element very different from canonical TTF-1 binding sites. Nuclear extracts from the head of mouse embryos form a retarded complex with NestBS of the same mobility of the extracts obtained from TTF1-expressing clones, which is either abolished or supershifted in the presence of two different antibodies recognizing the TTF-1 protein. Thus, the neuroepithelial marker nestin is a direct central nervous system-specific target gene of TTF-1, leading to the hypothesis that it might be the effector through which TTF-1 plays its role in the organogenesis of the forebrain.Thyroid transcription factor-1 (TTF-1), 1 also known as NKX2.1 and T/EBP, is a member of the NKX family of homeodomain-containing genes related to NK Drosophila genes (1). TTF-1 plays a fundamental role in the tissue-specific expression of several thyroid-specific (i.e. thyroglobulin, thyroperoxidase, thyrotropin receptor, and sodium iodide symporter) and lung-specific (i.e. surfactant proteins and the Clara cell secretory protein) genes (2-7). TTF-1 has been reported to be expressed also in the adult rat parathyroid cells and several other adult tissues (including skin, esophagus, retina, anterior pituitary, cerebellum, and hippocampus) (8,9). Recently, it has been shown that postnatal hypothalamic TTF-1 expression is strongly and transiently up-regulated immediately before puberty in female rats and is associated with the neuroendocrine process of female sexual development (10).TTF-1 is expressed at the onset of thyroid and lung organogenesis, and in restricted areas of the developing forebrain, namely within the diencephalon (i.e. in the hypothalamus and neurohypophysis) and the telencephalon (i.e. in the medial ganglionic eminence) (11). The crucial role exerted by TTF-1 in thyroid, lung, and ventral forebrain organogenesis has been directly demonstrated by the analysis of the phenotype of TTF-1 Ϫ/Ϫ mice. In fact, homozygous mutant mice were born dead and lacked completely the thyroid gland, lung parenchyma, and the entire pituitary, and extensive defects were found in the ventral region of the forebrain (12). In addition, it has been reported that, in TTF-1 knockout mice, a ventral-to-dorsal transformat...

show abstract

“…The column was equilibrated in buffer A (30 mM HEPES, 0.1 mM EGTA, 0.5 mM DTT, 0.5 mM PMSF) þ 80 mM NaCl and loaded with the nuclear extract prepared from HeLa cells overexpressing Pax 8. The nuclear extract, prepared according to Suzuki et al (1998), was diluted with buffer A to decrease the salt concentration to 80 mM. The column was washed with buffer A þ 80 mM NaCl and eluted with buffer A þ 1 M NaCl.…”

Section: Affinity Column and Band-shift Assaymentioning

confidence: 99%

Retinoblastoma protein acts as Pax 8 transcriptional coactivator

Miccadei¹,

Provenzano

Mojžíšek

et al. 2005

Oncogene

View full text Add to dashboard Cite

Control of cell proliferation and differentiation by the retinoblastoma protein (pRb) depends on its interactions with key cellular substrates. Available data indicate that pRb and the transcription factor Pax 8 play a crucial role in the differentiation of thyroid follicular cells. In this study, we show that pRb takes part in the complex assembled on the thyroperoxidase gene promoter acting as a transcriptional coactivator of Pax 8. Accordingly, pRb interacts with and potentiates Pax 8 transcriptional activity. In addition, we show that the downregulation of pRb gene expression, in thyrocytes, through RNA interference results in a reduction of the thyroperoxidase gene promoter activity mediated by the Pax 8-binding site. In agreement with these results and with the ability of the adenoviral protein E1A to bind pRb, we show that E1A downregulates Pax 8 activity and that such inhibition requires the E1A-Rb interaction. Furthermore, we show that the Pax 8/pRb synergy plays a role on the sodium/ iodide symporter gene expression as well.

show abstract

Thyroid Transcription Factor 1 Is Calcium Modulated and Coordinately Regulates Genes Involved in Calcium Homeostasis in C Cells

Cited by 74 publications

References 67 publications

Differential regulation of apical and basal iodide transporters in the thyroid by thyroglobulin

Differential regulation of apical and basal iodide transporters in the thyroid by thyroglobulin

Nestin Is a Neuroepithelial Target Gene of Thyroid Transcription Factor-1, a Homeoprotein Required for Forebrain Organogenesis

Retinoblastoma protein acts as Pax 8 transcriptional coactivator

Contact Info

Product

Resources

About