Thyroid signaling in immune organs and cells of the teleost fish rainbow trout (Oncorhynchus mykiss)

Quesada-García, Alba; Valdehíta, Ana; Kropf, Christian; Casanova-Nakayama, Ayako; Segner, Helmut; Navas, José M.

doi:10.1016/j.fsi.2014.03.016

Cited by 36 publications

(20 citation statements)

References 68 publications

(81 reference statements)

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“…Transcript changes for the thyroid receptors alpha and beta clearly differed between both treatments: lower levels of TBBPA up-regulated the thyroid receptor genes while PTU down-regulated these genes. These findings confirm the current knowledge about the effects of both substances on the fish thyroid system (De Wit et al, 2008;Quesada-García et al, 2014) and show that they were disrupting the thyroid system in a different, partly even contrasting manner. This was the initial starting point for our further investigations about the eye morphology and physiology of treated larvae.…”

Section: Discussionsupporting

confidence: 88%

Thyroid disruption in zebrafish (Danio rerio) larvae: Different molecular response patterns lead to impaired eye development and visual functions

et al. 2016

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The vertebrate thyroid system is important for multiple developmental processes, including eye development. Thus, its environmentally induced disruption may impact important fitnessrelated parameters like visual capacities and behaviour. The present study investigated the relation between molecular effects of thyroid disruption and morphological and physiological changes of eye development in zebrafish (Danio rerio). Two test compounds representing different molecular modes of thyroid disruption were used: propylthiouracil (PTU), which is an enzyme-inhibitor of thyroid hormone synthesis, and tetrabromobisphenol A (TBBPA), which interacts with the thyroid hormone receptors. Both chemicals significantly altered transcript levels of thyroid system-related genes (TR, TR, TPO, TSH, DIO1, DIO2 and DIO3) in a compound-specific way. Despite these different molecular response patterns, both treatments resulted in similar pathological alterations of the eyes such as reduced size, RPE cell diameter and pigmentation, which were concentration-dependent. The morphological changes translated into impaired visual performance of the larvae: the optokinetic response was significantly and concentrationdependently decreased in both treatments, together with a significant increase of light preference of PTU-treated larvae. In addition, swimming activity was impacted. This study provides first evidence that different modes of molecular action of the thyroid disruptors can be associated with uniform apical responses. Furthermore, this study is the first to show that pathological eye development, as it can be induced by exposure to thyroid disruptors, indeed translates into impaired visual capacities of zebrafish early life stages. Abstract:The vertebrate thyroid system is important for multiple developmental processes, including eye development. Thus, its environmentally induced disruption may impact important fitnessrelated parameters like visual capacities and behaviour. The present study investigated the relation between molecular effects of thyroid disruption and morphological and physiological changes of eye development in zebrafish (Danio rerio). Two test compounds representing different molecular modes of thyroid disruption were used: propylthiouracil (PTU), which is an enzyme-inhibitor of thyroid hormone synthesis, and tetrabromobisphenol A (TBBPA), which interacts with the thyroid hormone receptors. Both chemicals significantly altered transcript levels of thyroid system-related genes (TR, TR, TPO, TSH, DIO1, DIO2 and DIO3) in a compound-specific way. Despite these different molecular response patterns, both treatments resulted in similar pathological alterations of the eyes such as reduced size, RPE cell diameter and pigmentation, which were concentration-dependent. The morphological changes translated into impaired visual performance of the larvae: the optokinetic response was significantly and concentration-dependently decreased in both treatments, together with a significant increase of light preference of PT...

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Section: Discussionsupporting

confidence: 88%

Thyroid disruption in zebrafish (Danio rerio) larvae: Different molecular response patterns lead to impaired eye development and visual functions

et al. 2016

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“…There exist a few reviews on the influence of endogenous hormones on the fish immune system (Harris & Bird 2000), with a focus on the role of corticosteroids. Concerning environmental EDCs, up to now, mainly compounds with estrogenic, androgenic and thyroidal activities have been investigated (Rice & Xiang 2000;Segner et al 2006;Milla et al 2011;Quesada-Garc ıa et al 2014). Many immune disorders are rooted in the endocrine system due to the fact that the endocrine and the immune systems are intricately connected, with some of the immune and hormone factors having evolved within the same family of structurally related molecules ).…”

Section: Hormones/endocrine Disrupting Compounds (Edcs; 27%)mentioning

confidence: 99%

“…Another receptor pathway which appears to play a role in mediating effects of EDCs on the fish immune system is thyroid signaling via thyroid receptors a and b. In fact, the TRs are expressed in fish leukocytes, and, consequently, exposure of fish to thyroid disruptors can modulate transcript levels of diverse immune genes (Quesada-Garc ıa et al 2014;.…”

Section: Hormones/endocrine Disrupting Compounds (Edcs; 27%)mentioning

confidence: 99%

20 Years of fish immunotoxicology – what we know and where we are

Rehberger

Werner

Hitzfeld

et al. 2017

Critical Reviews in Toxicology

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Despite frequent field observations of impaired immune response and increased disease incidence in contaminant-exposed wildlife populations, immunotoxic effects are rarely considered in ecotoxicological risk assessment. The aim of this study was to review the literature on immunotoxic effects of chemicals in fish to quantitatively evaluate (i) which experimental approaches were used to assess immunotoxic effects, (ii) whether immune markers exist to screen for potential immunotoxic activities of chemicals, and (iii) how predictive those parameters are for adverse alterations of fish immunocompetence and disease resistance. A total of 241 publications on fish immunotoxicity were quantitatively analyzed. The main conclusions included: (i) To date, fish immunotoxicology focused mainly on innate immune responses and immunosuppressive effects. (ii) In numerous studies, the experimental conditions are poorly documented, as for instance age or sex of the fish or the rationale for the selected exposure conditions is often missing. (iii) Although a broad variety of parameters were used to assess immunotoxicity, the rationale for the choice of measured parameters was often not given, remaining unclear how they link to the suspected immunotoxic mode of action of the chemicals. (iv) At the current state of knowledge, it is impossible to identify a set of immune parameters that could reliably screen for immunotoxic potentials of chemicals. (v) Similarly, in fish immunotoxicology there is insufficient understanding of how and when chemical-induced modulations of molecular/cellular immune changes relate to adverse alterations of fish immunocompetence, although this would be crucial to include immunotoxicity in ecotoxicological risk assessment.

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“…Several disadvantages of these risk assessment studies according to the bioindicator level used were, however, pointed out as their inability to act as an early detection system that could be relied on as in the case of impending severe pollution crises in aquatic ecosystems. Thus, the use of biomarkers such as SCGE, AChE and EROD may provide an excellent nexus leading to unveil some significant clues to identify the causal mechanisms potentially responsible for any type of damage at molecular, cellular, and sub-cellular levels [35,[37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Ecological Risk Assessment of Urban Streams Using Fish Biomarkers of DNA Damage and Physiological Responses

Bae¹,

Atique²,

Yoon³

et al. 2020

Pol. J. Environ. Stud.

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Ecological risk assessment was carried out in two urban streams, viz. Gap Stream (GS) and Miho Stream (MS), in the Geum River Watershed during July 2013-October 2014. The techniques used in this study included multi-level fish biomarkers of DNA damage based on single-cell gel electrophoresis (SCGE) coupled with the study of physiological responses based on 7-ethoxyresorufin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities in fish species. Biomarker values of tail DNA (tDNA), tail length (T L), and tail extent moment (TEM) in impacted zone (I z) were 2.0-3.6-fold greater than in controls (C z). Nucleus image analysis showed that the nucleus had circular particle forms in the C z as compared with a longitudinal oval form with broken particles from the nucleus in the I z. Physiological response analysis of EROD and AchE activities indicated that their levels were higher in the I z than in the C z. Such DNA damages and greater physiological responses in the I z were attributed to chemical contaminants discharged from the wastewater disposal plants and industrial complex. This combination of DNA damage and physiological responses approach can be used as a key tool for early-warning detection of chemical contaminants and concomitant risks to the ecological health of urban streams.

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Thyroid signaling in immune organs and cells of the teleost fish rainbow trout (Oncorhynchus mykiss)

Cited by 36 publications

References 68 publications

Thyroid disruption in zebrafish (Danio rerio) larvae: Different molecular response patterns lead to impaired eye development and visual functions

Thyroid disruption in zebrafish (Danio rerio) larvae: Different molecular response patterns lead to impaired eye development and visual functions

20 Years of fish immunotoxicology – what we know and where we are

Ecological Risk Assessment of Urban Streams Using Fish Biomarkers of DNA Damage and Physiological Responses

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