Thyroid Hypocalcemic Principle and Recurrent Laryngeal Nerve Injury as Factors Affecting the Response to Parathyroidectomy in Rats1

Hirsch, Philip F.; Gauthier, Guy; Munson, Paul L.

doi:10.1210/endo-73-2-244

Cited by 357 publications

(76 citation statements)

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“…Calcitonin (CT), the hypocalcemic hypophosphatemic hormone [1,2], is the specific marker for medullary carcinoma of the thyroid (MCT) [3] a tumor existing either in sporadic or familial (autosomal dominant) form. The hormone is also produced ectopically by a large number of tumors thought to arise from cells sharing either common histochemical characteristics, the Apud concept [4], or the same embryological origin, the neural crest [5].…”

Section: Introductionmentioning

confidence: 99%

The complete sequence of human preprocalcitonin

Moullec¹,

et al. 1984

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DNA complementary to mRNA extracted from the thyroid glands of patients suffering from medullary carcinoma of the thyroid (MCT), a calcitonin‐producing tumour, was inserted in the Pst site of pBR 322 by G‐C tailing. The recombinant plasmids were used to transform Escherichia coli DP 50. Ampicillin‐resistant clones were screened using a 32P‐labelled cDNA to mRNA extracted from a case of MCT particularly rich in calcitonin (CT) mRNA. Positive clones were subsequently rescreened using a 32P poly(T) probe. Eighty clones were thus purified, and the inserts obtained by digestion with PstI were subjected to positive hybridization selection with subsequent translation in vitro. An insert stimulating synthesis of the protein and containing restriction sites compatible with the previously published complete sequence of calcitonin mRNA from rat was sequenced. This cDNA insert contained the entire coding region of 426 bp, 70 bp at the 5′‐end, and 295 bp upstream from the poly(A) tail. The complete amino acid sequence of human preprocalcitonin could thus be deduced.

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Section: Introductionmentioning

confidence: 99%

The complete sequence of human preprocalcitonin

Moullec¹,

et al. 1984

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“…Calcitonin, the hypocalcemic hypophosphatemic hormone (Copp et al, 1962;Hirsch et al, 1963), is involved in the regulation of calcium metabolism. The hormone is secreted by the C cells, localized in the thyroid in mammalia and in the ultimobranchial glands in lower vertebrates.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of the nucleotide sequence of chicken calcitonin mRNA: direct evidence for the expression of a lower vertebrate calcitonin-like gene in man and rat.

Lasmoles¹,

Jullienne²,

Day³

et al. 1985

The EMBO Journal

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Calcitonin shows considerable divergence in amino acid sequence between lower vertebrates and higher vertebrates. Immunoreactive salmon-like calcitonin molecules are present in the thyroid of man and rat. Elucidation of the almost complete sequence of chicken calcitonin mRNA revealed that the calcitonin precursor in chickens had the same organisation as in higher vertebrates (man and rat) but showed considerable differences in amino acid sequence. cDNA probes specific for chicken calcitonin mRNA hybridized to poly(A)-rich RNA extracted from a case of medullary carcinoma of the thyroid and from murine thyroid. These results suggest the expression in man and rat of a gene coding for an avian calcitoninlike precursor.

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“…Hirsch, Gauthier, and Munson (1963) made a second and highly significant observation. These investigators noted that parathyroidectomy by the Erdheim cautery technique, which involves hot wire cauterization of adjacent thyroid tissue in the rat, resulted in a much more rapid and profound hypocalcemia than simple surgical Parathyroidectomy.…”

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confidence: 89%

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

Oeltgen¹,

L'Heureux²

1975

Horm Metab Res

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Mitochondria, nu dei, and microsomes obtained from ovine thyroid slices wh ich had been incubated in Krebs-Ringer bicarbonate buffer containing 4.8, 11.5 or 26.0 mg% concentration of calcium were assayed for calcitonin activity in rats. The microsomal preparatioa was the only particulate fraction to exhibit marked hypocalcemic activity and the hormonal activity of this fraction was enhanced by a 26.0 mg% calcium concentration in the incubation medium. The identity of the hypocalcemic species in the microsomal fraction with a calcitonin preparation 01' known potency was established by polyacrylamide gel eletrophoresis and by isoelectric focusing. The ovine microsomal calcitonin was resolved into two pro tein bands with isoelectric points (pl's) of 3.49 and 3.84. Both of these protein peaks exhibited hypocalcemic activity. The porcine standard calcitonin was resolved into two protein bands with pl's of 3.70 and 3.94.

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Thyroid Hypocalcemic Principle and Recurrent Laryngeal Nerve Injury as Factors Affecting the Response to Parathyroidectomy in Rats1

Cited by 357 publications

References 0 publications

The complete sequence of human preprocalcitonin

The complete sequence of human preprocalcitonin

Elucidation of the nucleotide sequence of chicken calcitonin mRNA: direct evidence for the expression of a lower vertebrate calcitonin-like gene in man and rat.

Calcitonin Activity of Particulate Fractions of the Ovine Thyroid Gland

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