Dioxins [polychlorinated dibenzo-p-dioxins (PCDD), dibenzofurans (PCDF)] and polychlorinated biphenyls (PCB) are potentially hazardous compounds. Animal studies have demonstrated that PCDD, PCDF, and PCB can alter thyroid hormone homeostasis. We investigated thyroid hormone levels in 105 mother-infant pairs. To estimate maternal and infant exposure, four nonplanar PCB congeners were measured in maternal plasma during the last month of pregnancy and in umbilical cord plasma. Seventeen PCDD and PCDF congeners, three planar PCB congeners, and 23 nonplanar PCB congeners were measured in human milk. Higher PCDD, PCDF, and PCB levels in human milk, expressed as toxic equivalents, correlated significantly with lower plasma levels of maternal total triiodothyronine and total thyroxine, and with higher plasma levels of TSH in the infants in the 2nd wk and 3rd mo after birth. Infants exposed to higher toxic equivalents levels had also lower plasma free thyroxine and total thy-PCDD and PCDF, summarized as dioxins, are tricyclic aromatic compounds. Because the number of chlorine atoms can vary between one and eight, there are potentially 75 different PCDD and 135 PCDF congeners. They are mainly formed as by-products during the synthesis of organochlorine chemicals and during the combustion of municipal and hazardous waste (1). PCB are a compilation of 209 possible congeners with different chlorine substitutions. Because of their unique physical properties and chemical stability, mixtures were used for diverse