Thymus antibody-secreting cells possess an interferon gene signature and are preferentially expanded in young female mice

Pioli, KimAnh Trang; Lau, Kin H.; Pioli, Peter D.

doi:10.1016/j.isci.2023.106223

Cited by 5 publications

(34 citation statements)

References 107 publications

(166 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In support of the above, parabiosis and intravenous antibody labeling experiments in mice have indicated local production of THY ASCs ( 35 , 37 ). While these types of functional experiments are missing in humans, in vitro differentiation experiments using human samples have shown the ability of THY B cells to produce ASCs ( 34 , 36 ).…”

Section: Current Knowledge In the Fieldmentioning

confidence: 78%

“…In the THY, ASCs are incredibly rare and consist of less than 0.05% of total organ cellularity ( 37 ). Not surprisingly, the attempt to localize these cells within particular THY niches has been difficult.…”

Section: Current Knowledge In the Fieldmentioning

confidence: 99%

“…The chemokine cues required to localize ASCs within the THY have not been experimentally determined. However, THY ASCs do express CXCR4 and CXCR3 ( 35 , 37 ) and their respective ligands, CXCL12 and CXCL9/10/11, can be found in the THY ( 38 ). Important to note, CXCL12 is highly enriched in the THY cortex ( 39 ) whereas CXCL9/10/11 are found more so in the medulla ( 38 ) suggesting that these factors may compete with one another and dictate the local positioning of THY ASCs.…”

Section: Current Knowledge In the Fieldmentioning

confidence: 99%

“…Recent work which examined trafficking of CD8 T cells, demonstrated the loss of these cells in the THY of Cxcl10 -/- mice ( 40 ) supporting the importance of this chemokine in maintaining selected populations within the THY. Furthermore, increased expression of CD69 and CD44 has been observed on mouse ASCs from the THY when compared to those from the BM and SPL ( 35 , 37 ) suggesting that these cells possess a tissue residency phenotype ( 41 ) and remain segregated within the THY. Recent intravital imaging experiments demonstrated that BM ASCs were not completely sessile as they periodically migrated within the BM compartment ( 42 ).…”

Section: Current Knowledge In the Fieldmentioning

confidence: 99%

“…By disrupting these basic mechanisms and recapitulating various autoimmune-related pathologies, we have the potential to identify druggable targets/pathways. While the identification of THY ASCs first occurred at least as early as the 1960s ( 25 , 27 , 33 ), only recently has that there has been a push to study THY ASCs in both human and mouse species with the above goals in mind ( 34 – 37 ). This review summarizes the current state of the field regarding THY ASCs and discusses future directions in understanding the etiology and function of these cells.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Thymus antibody-secreting cells: once forgotten but not lost

Pioli

2023

Front. Immunol.

View full text Add to dashboard Cite

Antibody-secreting cells are essential contributors to the humoral response. This is due to multiple factors which include: 1) the ability to secrete thousands of antibodies per second, 2) the ability to regulate the immune response and 3) the potential to be long-lived. Not surprisingly, these cells can be found in numerous sites within the body which include organs that directly interface with potential pathogens (e.g., gut) and others that provide long-term survival niches (e.g., bone marrow). Even though antibody-secreting cells were first identified in the thymus of both humans and rodents in the 1960s, if not earlier, only recently has this population begun to be extensively investigated. In this article, we provide an update regarding the current breath of knowledge pertaining to thymus antibody-secreting cells and discuss the potential roles of these cells and their impact on health.

show abstract

Section: Current Knowledge In the Fieldmentioning

confidence: 78%

Section: Current Knowledge In the Fieldmentioning

confidence: 99%

Section: Current Knowledge In the Fieldmentioning

confidence: 99%

Section: Current Knowledge In the Fieldmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Thymus antibody-secreting cells: once forgotten but not lost

Pioli

2023

Front. Immunol.

View full text Add to dashboard Cite

show abstract

Retro-orbital CD45 antibody labeling to evaluate antibody-secreting cell trafficking in mice

Pioli¹,

Pioli²

2023

STAR Protocols

Self Cite

View full text Add to dashboard Cite

Jchain-DTRMice Allow for Diphtheria Toxin-Mediated Depletion of Antibody-Secreting Cells and Evaluation of Their Differentiation Kinetics

Pioli,

Ritchie,

Haq

et al. 2024

Preprint

View full text Add to dashboard Cite

Antibody-secreting cells (ASCs) are generated following B cell activation and constitutively secrete antibodies. As such, ASCs are key mediators of humoral immunity whether it be in the context of pathogen exposure, vaccination or even homeostatic clearance of cellular debris. Therefore, understanding basic tenants of ASC biology such as their differentiation kinetics following B cell stimulation is of importance. Towards that aim, we developed a mouse model which expresses simian HBEGF (a.k.a., diphtheria toxin receptor (DTR)) under the control of the endogenous Jchain locus (or J-DTR). ASCs from these mice expressed high levels of cell surface DTR and were acutely depleted following diphtheria toxin treatment. Furthermore, proof-of-principle experiments demonstrated the ability to use these mice to track ASC reconstitution following depletion in 3 distinct organs. Overall, J-DTR mice provide a new and highly effective genetic tool allowing for the study of ASC biology in a wide range of potential applications.

show abstract

Thymus antibody-secreting cells possess an interferon gene signature and are preferentially expanded in young female mice

Cited by 5 publications

References 107 publications

Thymus antibody-secreting cells: once forgotten but not lost

Thymus antibody-secreting cells: once forgotten but not lost

Retro-orbital CD45 antibody labeling to evaluate antibody-secreting cell trafficking in mice

Jchain-DTRMice Allow for Diphtheria Toxin-Mediated Depletion of Antibody-Secreting Cells and Evaluation of Their Differentiation Kinetics

Contact Info

Product

Resources

About