Thymosin β4 Reduces H2O2 Induced Oxidative Stress in MC3T3-E1 Cells on Titanium Surface

Choi, Byeong-Dae; Lim, Do-Seon; Lee, Seungyeon; Nho, Tae-Hee; Jeong, Soon‐Jeong; Ko, Yeong-Mu; Gang, Sung-Nam; Kim, Young-Joon; Piao, Xing-Hui; Jeong, Moon‐Jin

doi:10.1166/jnn.2018.14865

Cited by 7 publications

(4 citation statements)

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“…Oxidative stress greatly influences the viability, proliferation, and survival of osteocytes 29 . In this study, we used 0.2 mM H 2 O 2 to generate a model of oxidative damage according to a previous study 30 . We observed that after treatment with 0.2 mM H 2 O 2 , the viability and osteogenic differentiation capacity of cells was decreased.…”

Section: Discussionmentioning

confidence: 99%

Protection of Icariin Against Hydrogen Peroxide‐Induced MC3T3‐E1 Cell Oxidative Damage

Sun

Wang

2021

Orthopaedic Surgery

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Objective The aim of the present study was to evaluate the potential protective mechanism of icariin against oxidative damage caused by hydrogen peroxide in MC3T3‐E1 cells. Methods MC3T3‐E1 cells were treated with different concentrations of icariin to explore the optimal dose of icariin. MC3T3‐E1 cells were divided into groups treated with various concentrations of hydrogen peroxide (H2O2; 0, 0.1, 0.2, 0.5, 1, and 2 mM) for 24 h to induce oxidative damage and cell viability was assessed by Cell Counting Kit‐8 (CCK‐8) assay. Then, cells were divided into five groups: control, H2O2 (0.2 mM), icariin (0.1 μM) and H2O2 (0.2 mM), + icariin (0.1 μM). Cell viability was detected by CCK‐8 assay. In addition, the content of glutathione and superoxide dismutase and the activity level of malondialdehyde in these treatment groups were determined. Alkaline phosphatase (ALP) and alizarin red S (ARS) staining were also performed to measure the early and late osteogenesis, respectively. Protein expression of β‐catenin and cyclin D1 was measured by western blot assay. Then, we used an antagonist of Wnt/β‐catenin signaling pathway (DKK‐1) and western blot analysis to further explore potential mechanism. Results After 24 h of exposure to 0.2 mM H2O2, the viability of MC3T3‐E1 cells was significantly decreased compared to that of the control cells. We first found that icariin can promote cell proliferation of MC3T3‐E1 cells in a dose‐dependent manner, with the dosage 0.1 μM showing the best pro‐proliferative effect. Furthermore, icariin could promote the protein expression of OSX and RUNX2. The results showed that icariin can reverse the inhibitory osteogenic effects of MC3T3‐E1 caused by H2O2. In addition, icariin could increase the Wnt‐signaling related proteins. The results showed that MC3T3‐E1 cells in the H2O2 (0.2 mM) + icariin (0.1 μM) + Wnt‐signaling antagonist (DKK‐1) group had weaker ALP and ARS staining compared with that observed in the control and H2O2 (0.2 mM) + icariin (0.1 μM) groups. The ALP activity and calcium content were decreased in the 0.2 mM H2O2 + 0.1 μM icariin + DKK‐1 group compared to that observed in the 0.2 mM H2O2 + 0.1 μM icariin group. Conclusion The results showed that icariin can increase the viability of MC3T3‐E1 cells, reverse the oxidative stress induced by H2O2 and protect MC3T3‐E1 cells against H2O2‐induced inhibition of osteogenic differentiation, which may occur through the Wnt/β‐catenin signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Protection of Icariin Against Hydrogen Peroxide‐Induced MC3T3‐E1 Cell Oxidative Damage

Sun

Wang

2021

Orthopaedic Surgery

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“…Furthermore, Tβ4 treatment has been shown to reduce the release of ROS and inflammation. 40 In addition, growing evidence shows that Tβ4 is involved in antioxidant, anti-inflammatory and antifibrotic responses to liver injury. 18 , 39 , 41 , 42 It has also been reported that the serum Tβ4 concentration is closely related to the severity of hepatic injury.…”

Section: Discussionmentioning

confidence: 99%

Hypomethylation of thymosin β4 promoter is associated with glucocorticoid therapy in patients with acute-on-chronic hepatitis B-induced liver failure

Wang

et al. 2022

International Health

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Background We aimed to determine whether the methylation status of thymosin β4 (Tβ4) promoter reflects the severity of acute-on-chronic hepatitis B liver failure (ACHBLF) and whether glucocorticoids affect this status. Methods Fifty-six patients with ACHBLF, 45 with chronic hepatitis B (CHB) and 32 healthy controls (HCs), were retrospectively enrolled. Methylation-specific PCR and real-time PCR were used to detect Tβ4 methylation frequency and mRNA level. The expression of Tβ4 was measured before and after glucocorticoid treatment in patients with ACHBLF. Clinical and laboratory parameters were obtained. Results Tβ4 mRNA expression of patients with ACHBLF was lower than in patients with CHB or HCs, but the methylation frequency was higher. Tβ4 promoter methylation frequency was correlated with serum total bilirubin, prothrombin activity and model for end-stage liver disease score. Moreover, Tβ4 promoter methylation frequency decreased and demethylation occurred during glucocorticoid therapy. After glucocorticoid therapy, Tβ4 mRNA expression and liver function were better in patients with low levels of methylation than in those with higher levels. After 90 d, the survival of patients with low levels of methylation was significantly higher than those with high levels. Conclusions Patients with ACHBLF who have low levels of Tβ4 methylation may show a more favorable response to glucocorticoid treatment.

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“…A reduction in scar tissue has been observed by multiple research groups in different animal models of injury, including excisional wounds, myocardial infarct, stroke, and fibrosis of the lung, liver, and kidney [44][45][46][47][48][49][50][51] (table 2). Thymosin ␤4 reduces oxidative stress by targeting anti-oxidative genes [52][53][54][55][56][57]. It reduced toxicity by inhibiting inducible nitric oxide synthetase (iNOS) and cyclooxygenase-2, resulting in decreased reactive oxygen species (ROS), decreased secretion of NO, and decreased prostaglandins.…”

Section: Thymosin ␤4 Regenerative Activitiesmentioning

confidence: 99%

Thymosin β4: potential to treat epidermolysis bullosa and other severe dermal injuries

Yang¹,

Kang²,

Sung³

et al. 2019

European Journal of Dermatology

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is a naturally-occurring regenerative protein present in almost all cells and body fluids, including wound fluid. In multiple preclinical injury models, it promotes dermal repair and tissue regeneration. Thymosin ␤4 acts by increasing keratinocyte/epithelial cell migration, angiogenesis, and cell survival, and by decreasing inflammation, apoptosis, and scarring. It also modulates cytokines, including those that cause itching. Thymosin ␤4 promotes faster repair in various chronic human wounds, including pressure ulcers, stasis ulcers, and epidermolysis bullosa lesions. The faster healing time with increased keratinocyte migration and angiogenesis and reduction in both inflammation and scarring are especially important for epidermolysis bullosa patients who suffer from slow healing and inflammation that leads to itching, infections, pain, fluid loss, scarring, and tissue damage. These multiple mechanisms of action support thymosin ␤4's role in accelerating dermal repair and suggest the potential to treat various types of severe wounds, including epidermolysis bullosa patients who suffer from frequent blistering wounds that can be life threatening. There is an urgent need at this time to develop a therapeutic, such as thymosin ␤4, for epidermolysis bullosa. Despite progress in gene/stem cell therapy, there is no cure for this disease and careful wound management is the standard of care.

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Thymosin β4 Reduces H₂O₂ Induced Oxidative Stress in MC3T3-E1 Cells on Titanium Surface

Cited by 7 publications

References 0 publications

Protection of Icariin Against Hydrogen Peroxide‐Induced MC3T3‐E1 Cell Oxidative Damage

Protection of Icariin Against Hydrogen Peroxide‐Induced MC3T3‐E1 Cell Oxidative Damage

Hypomethylation of thymosin β4 promoter is associated with glucocorticoid therapy in patients with acute-on-chronic hepatitis B-induced liver failure

Thymosin β4: potential to treat epidermolysis bullosa and other severe dermal injuries

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