Thymosin beta-4 overexpression correlates with high-risk groups in gastric gastrointestinal stromal tumors: A retrospective analysis by immunohistochemistry

Şahin, Sevinç; Ekinci, Özgür; Seçkin, Selda; Dursun, Ayşe

doi:10.1016/j.prp.2017.07.005

Cited by 2 publications

(2 citation statements)

References 47 publications

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“…Studies have shown that thymosin beta-4 promotes tumor metastasis and is highly expressed in pancreatic cancer, esophageal cancer, colon cancer, and gastric cancer [34][35][36][37]. A study by Sahin et al [38] also showed that thymosin beta-4 was overexpressed in the gastric mucosa of patients with gastrointestinal stromal tumors (GISTs). Overexpression of thymosin beta-4 in the gastric mucosa was related to tumor progression and poor prognosis in the GIST patients.…”

Section: Discussionmentioning

confidence: 99%

Acupuncture Regulates Serum Differentially Expressed Proteins in Patients with Chronic Atrophic Gastritis: A Quantitative iTRAQ Proteomics Study

Yang

Liu

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Objective. To identify differentially expressed proteins (DEPs) in sera of patients with chronic atrophic gastritis (CAG) using isobaric tags for relative and absolute quantitation (iTRAQ) and to explore acupuncture’s mechanism in CAG. Methods. Peripheral sera from 8 healthy volunteers (HC), 8 chronic nonatrophic gastritis (NAG) patients, 8 CAG patients, and 8 CAG patients who underwent acupuncture treatment (CAG + ACU) were collected followed by labeling with iTRAQ reagent for protein identification and quantification using two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS). Representative DEPs were selected through bioinformatics, and proteins were verified by enzyme-linked immunosorbent assay (ELISA). Results. A total of 4,448 unique peptides were identified, corresponding to 816 nonredundant proteins. A 1.4-fold difference was used as the threshold. Compared with the HC group, 75 and 106 DEPs were identified from CAG and NAG groups, respectively. Compared with the CAG group, 110 and 66 DEPs were identified from the NAG and CAG + ACU groups, respectively. The DEPs were mainly involved in protein binding and the Notch signaling pathway-related proteins, and the upregulated proteins included actin-binding proteins (thymosin beta-4, tropomyosin-4, profilin-1, transgelin-2), while the downregulated proteins included Notch2 and Notch3. After acupuncture, the expression of these proteins in CAG patients was less differentiated from that in healthy people. The level of the above 6 proteins were verified by ELISA, and the results were similar to the results of iTRAQ analysis. Conclusions. Actin-binding proteins and Notch signaling pathway-related proteins were correlated with the development and progression of CAG and thus are potential diagnostic markers for CAG. Acupuncture may play a role in regulating actin-binding proteins and Notch signaling pathway-related proteins to play a therapeutic role in CAG.

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Section: Discussionmentioning

confidence: 99%

Acupuncture Regulates Serum Differentially Expressed Proteins in Patients with Chronic Atrophic Gastritis: A Quantitative iTRAQ Proteomics Study

Yang

Liu

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…In gliomas, expression of Tβ4 was positively correlated with the tumor grade and silencing Tβ4 inhibited invasion, migration, and enhanced survival of mice by regulating the transforming growth factor beta (TGFβ) and p53 signaling networks [42]. Gastrointestinal stromal tumors showed increased expression of Tβ4 and vascular endothelial growth factor (VEGF), suggesting an aggressive tumor behavior [43]. In breast cancer, overexpression of Tβ4 was strongly associated with expression of HIF-1α, HIF-2α, and VEGF-A [44].…”

Section: Thymosin β4 In Cancermentioning

confidence: 99%

Cytoskeletal Remodeling in Cancer

Aseervatham

2020

Biology

100

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Successful metastasis depends on cell invasion, migration, host immune escape, extravasation, and angiogenesis. The process of cell invasion and migration relies on the dynamic changes taking place in the cytoskeletal components; actin, tubulin and intermediate filaments. This is possible due to the plasticity of the cytoskeleton and coordinated action of all the three, is crucial for the process of metastasis from the primary site. Changes in cellular architecture by internal clues will affect the cell functions leading to the formation of different protrusions like lamellipodia, filopodia, and invadopodia that help in cell migration eventually leading to metastasis, which is life threatening than the formation of neoplasms. Understanding the signaling mechanisms involved, will give a better insight of the changes during metastasis, which will eventually help targeting proteins for treatment resulting in reduced mortality and longer survival.

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Thymosin beta-4 overexpression correlates with high-risk groups in gastric gastrointestinal stromal tumors: A retrospective analysis by immunohistochemistry

Cited by 2 publications

References 47 publications

Acupuncture Regulates Serum Differentially Expressed Proteins in Patients with Chronic Atrophic Gastritis: A Quantitative iTRAQ Proteomics Study

Acupuncture Regulates Serum Differentially Expressed Proteins in Patients with Chronic Atrophic Gastritis: A Quantitative iTRAQ Proteomics Study

Cytoskeletal Remodeling in Cancer

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