Thymosin ?4 induced phenotypic changes in Molt-4 leukemic cell line

Kokkinopoulos, D.; Perez, Sonia A.; Papamichail, Michail

doi:10.1007/bf00320927

Cited by 16 publications

(6 citation statements)

References 28 publications

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“…It was reported that the mRNA encoding Tβ4 was increased rapidly in tumor cells and the overexpression of Tβ4 could promote the cell grow and migrate [ 39 ]. However, application of β-thymosin could not repair the damaged skin of animals [ 40 ], and the β-thymosin could turn tumor cells into normal cells [ 41 ], indicating it has no or even inhibitory effects on cell proliferation. In this study, the BmTHY1 seems to have stronger effects to stimulate cell proliferation than BmTHY2 ( Fig 8 ), although both have similar stimulating effects on cell migration ( Fig 8 ), which is consistent with Tβ4’s role in the regulation of G-actin polymerization and depolymerization [ 9 , 10 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular and Physiological Characterization of Two Novel Multirepeat β-Thymosins from Silkworm, Bombyx mori

Kang

et al. 2015

PLoS ONE

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β-thymosin plays important roles in the development of the lymphatic system and the central nervous system in vertebrates. However, its role and function in invertebrates remain much less explored. Here, we firstly isolated a gene encoding β-thymosin in silkworm (Bombyx mori L.). Interestingly, this gene encodes two polypeptides, named as BmTHY1 and BmTHY2, via two different modes of RNA splicing. The recombinant proteins fused with an N-term GST tag were over-expressed in Escherichia coli (E. coli) and further purified to near homogenity to prepare mouse antibodies. The Western blot analysis showed that these proteins were expressed in various tissues and organs, as well as in different developmental stages. Amazingly, the expression of BmTHY2 was hugely increased during the pupae stage, indicating a specialized role in this period. The expression of these proteins was gradually decreased in BmN cells infected by BmNPV, suggesting they may play different roles in the virus infection. In addition, both BmTHY1 and BmTHY2 can interact with 14-3-3 of silkworm and Ubiquitin of BmNPV as shown by GST pull down and Co-IP assays, consistent with their roles in the regulation of the development of nervous system.

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Section: Discussionmentioning

confidence: 99%

Molecular and Physiological Characterization of Two Novel Multirepeat β-Thymosins from Silkworm, Bombyx mori

Kang

et al. 2015

PLoS ONE

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“…It is a 43-aminoacid polypeptide that was originally described as a thymic maturation factor (Low et al, 1981). It was reported that Tb4 could suppress the mixed lymphocyte proliferative response, induce the expression of terminal deoxynucleotidyl transferase in T cells, inhibit the migration of macrophages in vitro and induce the phenotypic changes in T cell line (Baxevanis et al, 1987;Kokkinopoulos et al, 1985). Recently, it was reported that Tb4 promotes angiogenesis, wound repair, and hair follicle development (Philp et al, 2004); stimulates tumor metastasis by activating cell migration and angiogenesis (Cha et al, 2003;Liu et al, 2003;Wang et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Thymosin β4 and AcSDKP inhibit the proliferation of HL‐60 cells and induce their differentiation and apoptosis

Huang

Wang

2006

Cell Biology International

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Our previous works have shown that bone marrow stromal cells secrete thymosin beta4 (Tbeta4) and AcSDKP. Tbeta4 and AcSDKP are existed in the conditioned medium of bone marrow endothelial cells. They exerted inhibitory effects on hematopoietic cells and then had protective effect on the early hematopoietic cells, which were cultured in the presence of hematopoietic stimulators. Thymosin beta4 consists of 43 peptides with a molecular weight of 4963. It contains at its N-terminal end the sequence of the acetylated tetrapeptide Ac-N-Ser-Asp-Lys-Pro (AcSDKP). This study was performed to evaluate the effect of Tbeta4 and AcSDKP on the growth of HL-60 cells. It was showed that Tbeta4 (10(-11)-10(-7)mol/L) and AcSDKP (10(-11)-10(-7)mol/L) had the dose-dependent inhibitory effect on the proliferation of HL-60 cells. Based on cell morphology and NBT reduction, Tbeta4 and AcSDKP induced differentiation of HL-60 cells. Morphologic and DNA fragment analysis proved that Tbeta4 and AcSDKP induced apoptosis of HL-60 cells. In order to analyze the mechanism of the effects of Tbeta4 and AcSDKP, intracellular free Ca(2+) concentration ([Ca(2+)](i)) of HL-60 leukemic cells was tested and Atlas cDNA Expression Array was performed. The results showed that Tbeta4 and AcSDKP could increased [Ca(2+)](i) by stimulating the release of Ca(2+) from intracellular Ca(2+) pool. Moreover, AcSDKP could also elicit a potent extracelluar calcium influx in HL-60 cells. Tbeta4 could also change apoptotic-related gene expression in leukemic cells, and resulted in the inhibition of proliferation and induction of differentiation and apoptosis of leukemic cells.

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“…The peptide is widely distributed in numerous tissues (Horecker & Morgan, 1981; Goodall et al, 1983;Hannappel, 1986) and in recent years different /3-thymosins were isolated from many species ranging from sea urchin (Schmid et al, 1989) to mammals (Hannappel, 1986). Thymosin /34 has been reported to induce terminal deoxynucleotidyl transferase activity in vivo and in vitro (Low et al, 1981), inhibit the migration of guinea pig peritoneal macrophages (Thurman et al, 1981), stimulate the hypothalamic secretion of luteinizing hormone releasing factor (Rebar et al, 1981), or induce phenotypic changes in the Molt-4 leukemic cell line (Kokkinopoulos et al, 1985).…”

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confidence: 99%

Solution conformation of thymosin .beta.4: a nuclear magnetic resonance and simulated annealing study

Zarbock¹,

Oschkinat²,

Hannappel³

et al. 1990

Biochemistry

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The conformation of the polypeptide thymosin beta 4 in solutions of 60% (v/v) trifluoroethanol-d3 and 50% (v/v) hexafluoroisopropyl-d2 alcohol in water is investigated by nuclear magnetic resonance (NMR) spectroscopy. Under these conditions thymosin beta 4 adopts an ordered structure. By use of a combination of two-dimensional NMR techniques, the 1H NMR spectrum of thymosin beta 4 is assigned. A set of 180 approximate interproton distance constraints is derived from nuclear Overhauser enhancement (NOE) measurements. These, together with 33 phi constraints obtained for JNH alpha coupling data and the 23 psi dihedral angles identified on the basis of the pattern of short-range NOEs, form the basis of a three-dimensional structure determination by dynamical simulated annealing. The calculations are carried out starting from three initial structures, an alpha-helix, an extended beta-strand, and a mixed alpha/beta structure. Ten independent structures are computed from each starting structure by using different random number seeds for the assignments of the initial velocities. All 30 calculated structures satisfy the experimental constraints, display very small deviations from idealized covalent geometry, and possess good nonbonded contacts. Analysis of the 30 converged structures indicates that there are two helical regions extending from residues 4-16 and from residues 30-40, which are well defined both in terms of atomic root mean square differences and backbone torsion angles. For the two helical regions individually the average backbone rms difference between all pairs of structures is approximately 2 A. The two helices exhibit typical amino acid preferences for specific locations at the ends of helices.(ABSTRACT TRUNCATED AT 250 WORDS)

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Thymosin ?4 induced phenotypic changes in Molt-4 leukemic cell line

Cited by 16 publications

References 28 publications

Molecular and Physiological Characterization of Two Novel Multirepeat β-Thymosins from Silkworm, Bombyx mori

Molecular and Physiological Characterization of Two Novel Multirepeat β-Thymosins from Silkworm, Bombyx mori

Thymosin β4 and AcSDKP inhibit the proliferation of HL‐60 cells and induce their differentiation and apoptosis

Solution conformation of thymosin .beta.4: a nuclear magnetic resonance and simulated annealing study

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