Thymoquinone: the Bioactive Component of Nigella Sativa

Güllü, Elif Bacak; Avcı, Gülcan

doi:10.5578/kvj.5251

Cited by 16 publications

(5 citation statements)

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“…TQ also has inhibitory effects on the proliferation of many types of cancer cells (Bacak Güllü & Avcı, 2013). In the literature review, some studies have been found on the quality of semen after freezing and thawing with semen extenders supplemented with TQ or N. sativa oil on ram, buck and buffalo semen (Awan et al, 2018;Miah et al, 2018;Shikh Maidin et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…TQ is known to have anti‐inflammatory, antioxidant, neuroprotection (Radad et al, 2009) properties, as well as a recovery effect against oxidative stress induced in neuropathy (Hamdy & Taha, 2009). TQ also has inhibitory effects on the proliferation of many types of cancer cells (Bacak Güllü & Avcı, 2013). In the literature review, some studies have been found on the quality of semen after freezing and thawing with semen extenders supplemented with TQ or N. sativa oil on ram, buck and buffalo semen (Awan et al, 2018; Miah et al, 2018; Shikh Maidin et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

et al. 2022

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The aim of this study was to determine the effects of thymoquinone (TQ), which is the most essential active compound of Nigella sativa, on the spermatological parameters of ram semen during cryopreservation. Ejaculates were collected from five Sonmez rams using an artificial vagina and extended with Tris‐based extender not containing TQ (control, 0 μg/ml TQ) and containing 10, 25, 50 and 100 μg/ml TQ. The extended semen samples were equilibrated in a + 4°C cold cabinet for 2 h. After 2 h, the samples were loaded into 0.25 ml French straws. The straws were frozen by liquid nitrogen vapour and stored in a liquid nitrogen container (−196°C). The frozen straws were thawed in a water bath (37°C for 30 s) and evaluated in terms of motility characteristics, plasma membrane and acrosome integrity, mitochondrial reactive oxygen species levels, lipid peroxidation levels, DNA damage and biochemical alterations (oxidative stress index, malondialdehyde and glutathione). TQ100 had higher total motility (53.59 ± 3.01) and progressive motility (19.84 ± 1.44; not significantly different from TQ25 and TQ50) compared to the control and TQ10 (p ˂ 0.05). According to the results of the analyses on motility characteristics, there were significant differences between the groups in terms of curvilinear velocity (VCL), amplitude of lateral head displacement (ALH) and linearity (LIN; p ˂ 0.05). The highest DNA damage was detected in the control group (p ˂ 0.05). TQ50 had higher plasma membrane and acrosome integrity (59.56 ± 5.92) compared to the control and TQ25 (p < 0.05) but not significantly different from TQ10 and TQ100. The lowest mitochondrial reactive oxygen species levels were detected in TQ50 and TQ100 (p ˂ 0.05). There were no significant differences among the groups in terms of their oxidative stress index, lipid peroxidation, malondialdehyde and glutathione levels (p > 0.05). According to the results, it could be concluded that supplementing 50 or 100 μg/ml TQ to Tris extenders that were used for ram semen cryopreservation showed a positive effect on motility, plasma membrane integrity and acrosome integrity, and it reduced DNA damage and mitochondrial reactive oxygen species levels.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

et al. 2022

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“…Although black cumin shows many differences in agricultural and quality characteristics according to the regions where it is cultivated, generally in the structure of the seed, it contains 0.4-0.45% essential oil, 32-40% fatty oil, 16-19.9% protein, 33.9% carbohydrates, 5.5% saponins, alkaloids and fibers, 1.79%-3.44% tannins and minerals. While there are unsaturated fatty acids such as linoleic acid, oleic acid and linolenic acid in its fatty oil, it contains palmitic acid, stearic acid and myristic acid from saturated fatty acids (Güllü and Avcı, 2013). Black cumin essential oil contains bioactive substances such as thymoquinone, p-cymene, nigellonethyllinoleate, α-thujen, thymol, α-pinene, carvacrol, trans-anethol, β-pinene.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of some black cumin seed (Nigella sativa L.) genotypes in terms of quality parameters at different planting periods under Mardin ecological conditions

Güneş¹,

Tonçer²

2023

Rev. Invest. Univ. Quindío

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This research was carried out with 3 repetitions according to the randomized blocks trial design in winter and summer planting periods of 2018-2019 and 2019-2020 in order to determine the agronomic and quality characteristics of some black cumin genotypes at different sowing periods under Mardin ecological conditions. Çameli variety and 18 different black cumin seed genotypes were used in the study and some quality parameters were examined. According to the results obtained from the research, it was determined that fatty oil ratio varied between 32.45-39.02% and fatty oil yield varied between 12.94-42.72 kg da-1 in winter planting. In summer planting; fatty oil ratio ranged between 33.99-38.13% and fatty oil yield ranged between 9.69-25.18 kg da-1. As a result of the study, it was determined that Eskişehir-5 (G9) genotype performed better than other varieties and genotypes in terms of fatty oil yield under Mardin ecological conditions. Due to the early increase in temperature in Mardin conditions, quality parameters decreased in summer planting compared to winter planting. Therefore, winter planting is recommended as the most suitable planting time for black cumin cultivation under Mardin conditions.

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“…TQ is the most important bioactive ingredient found in black cumin oil (Nigella sativa). Black cumin and TQ were reported to have anticancerogenic, antiulcerogenic, antiinfl ammatory and antioxidant effects (6). Their favourable effects on cardiac injury induced by certain medications (7) (8) and ischemia-reperfusion (9) have been previously reported; however, very few studies have reported the protective effects of TQ on the heart against DXR-induced toxicity (10,11).…”

Section: Introductionmentioning

confidence: 99%

The effects of melatonin and thymoquinone on doxorubicin-induced cardiotoxicity in rats

PEHLİVAN>¹,

Durdağı²,

Oyar³

et al. 2020

BLL

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OBJECTIVES: This study aims to investigate the protective effects of thymoquinone and melatonin on the heart against doxorubicin-induced cardiotoxicity in rats. BACKGROUND: Melatonin and thymoquinone may play an important role in cardiotoxicity. METHODS: The subjects were divided into four groups: Control (physiological serum on 5th day), Doxorubicin (DXR), Doxorubicin+Melatonin (DXR+MEL, 10 mg/kg melatonin, intraperitoneally), and Doxorubicin+Thymoquinone (DXR+TQ, 50 mg/kg thymoquinone, orally). On the 5th day of the experiment, all groups were injected with 45 mg/kg DXR into the tail vein. On the 8th day of the experiment, ECG recordings were performed under anaesthesia. RESULTS: Thymoquinone reduced the PR, QRS and QTc intervals, which were increased by DXR, while melatonin only reduced the QTc interval. Melatonin had a protective effect against the histopathological changes induced by DXR, while TQ did not demonstrate such an effect. DXR increased CK-MB, IL-6, MDA, IL-1, IL-18 levels and decreased SOD in the cardiac tissue. MEL reduced the levels of CK-MB, MDA, NO, SOD, IL-1, IL-6, IL-18. Meanwhile, TQ only reduced CK-MB, IL-1 and IL-18. CONCLUSION: Our study showed that DXR induces cardiac injury and that melatonin improves biochemical parameters and offers histological protection; while thymoquinone improves ECG parameters and causes partial recovery of biochemical parameters (Tab. 4, Fig. 2, Ref. 41).

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Thymoquinone: the Bioactive Component of Nigella Sativa

Cited by 16 publications

References 53 publications

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

Thymoquinone improves motility, plasma membrane integrity and DNA integrity of frozen–thawed ram semen

Evaluation of some black cumin seed (Nigella sativa L.) genotypes in terms of quality parameters at different planting periods under Mardin ecological conditions

The effects of melatonin and thymoquinone on doxorubicin-induced cardiotoxicity in rats

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