Thymoquinone suppresses growth and induces apoptosis via generation of reactive oxygen species in primary effusion lymphoma

Hussain, Azhar R.; Ahmed, Maqbool; Ahmed, Suhaib; Manogaran, Pulicat; Platanias, Leonidas C.; Alvi, Syed N.; Al‐Kuraya, Khawla S.; Uddin, Shahab

doi:10.1016/j.freeradbiomed.2010.12.034

Cited by 90 publications

(72 citation statements)

References 47 publications

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“…Since then, increasing numbers of studies have focused on TQ in cancer therapy. Numerous in vitro and in vivo studies have investigated the antitumor activity of TQ in several types of cancer (6,14,19,(27)(28)(29)33). In tumor protein p53-null myeloblastic leukemia HL-60 cells, TQ induced apoptosis through an intrinsic signaling pathway (34).…”

Section: Discussionmentioning

confidence: 99%

“…In human multiple myeloma cells, TQ inhibits proliferation, induces apoptosis and exerts a chemosensitization effect through suppressing signal transducer and activator of transcription 3 (acute-phase response factor) activation (35), and decreases F-actin polymerization and Bcl-2/Bcl-2 like 1 expression (36). TQ-induced apoptosis has been indicated to be mediated by reactive oxygen species generation (29,33,37) and the mitogen-activated protein kinase 14 signaling pathway (33). In 2004, Gali-Muhtasib et al (38) reported that TQ triggered apoptotic cell death in human colorectal cancer cells via a p53-dependent mechanism, and in 2008, Gali-Muhtasib et al (31) demonstrated that TQ triggered inactivation of the stress response pathway sensor checkpoint kinase 1 and contributed to apoptosis in colorectal cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown the antitumor and anticarcinogenic activities of TQ in numerous types of cancer, including breast cancer, glioblastoma and lymphoma (27)(28)(29). In order to elucidate the molecular mechanisms underlying the function of TQ, the effect of TQ on colon cancer cells was examined in the present study.…”

Section: Effect Of Tq On the Viability Of Colon Cancer Cellsmentioning

confidence: 99%

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Thymoquinone chemosensitizes colon cancer cells through inhibition of NF-κB

2016

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Abstract. In the present study, the effects and molecular mechanisms of thymoquinone (TQ) on colon cancer cells were investigated. Cell viability was determined using a Cell Counting Kit-8 assay, and the results revealed that treatment with TQ significantly decreased cell viability in COLO205 and HCT116 cells in a dose-dependent manner. TQ treatment additionally sensitized COLO205 and HCT116 cells to cisplatin therapy in a concentration-dependent manner. To investigate the molecular mechanisms of TQ action, western blot analysis was used to determine the levels of phosphorylated p65 and nuclear factor-κB (NF-κB)-regulated gene products vascular endothelial growth factor (VEGF), c-Myc and B-cell lymphoma 2 (Bcl-2). The results indicated that TQ treatment significantly decreased the level of phosphorylated p65 in the nucleus, which indicated the inhibition of NF-κB activation by TQ treatment. Treatment with TQ also decreased the expression levels of VEGF, c-Myc and Bcl-2. In addition, the inhibition of NF-κB activation with a specific inhibitor, pyrrolidine dithiocarbamate, potentiated the induction of cell death and caused a chemosensitization effect of TQ in colon cancer cells. Overall, the results of the present study suggested that TQ induced cell death and chemosensitized colon cancer cells by inhibiting NF-κB signaling.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Effect Of Tq On the Viability Of Colon Cancer Cellsmentioning

confidence: 99%

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Thymoquinone chemosensitizes colon cancer cells through inhibition of NF-κB

2016

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“…Nigella sativa and its constituents TQ showed vital effects as anticancer and also trigger apoptotic cell death in human colorectal cancer via a p53-dependent mechanism [55, 64]. A study demonstrate that Thymoquinone(TQ) treatment shows the down-regulation of constitutive activation of AKT via generation of reactive oxygen species (ROS) and it also causes conformational changes in Bax protein [65]. TQ also causes apoptosis in HEp-2 human laryngeal carcinoma cells through activation of caspase-3 and inhibitors of caspases significantly reduced the TQ-induced apoptosis [66].…”

Section: The Implications Of N Sativa In Cancer Therapymentioning

confidence: 99%

Therapeutic Implications of Black Seed and Its Constituent Thymoquinone in the Prevention of Cancer through Inactivation and Activation of Molecular Pathways

Rahmani

Alzohairy

Khan

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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The cancer is probably the most dreaded disease in both men and women and also major health problem worldwide. Despite its high prevalence, the exact molecular mechanisms of the development and progression are not fully understood. The current chemotherapy/radiotherapy regime used to treat cancer shows adverse side effect and may alter gene functions. Natural products are generally safe, effective, and less expensive substitutes of anticancer chemotherapeutics. Based on previous studies of their potential therapeutic uses, Nigella sativa and its constituents may be proved as good therapeutic options in the prevention of cancer. Black seeds are used as staple food in the Middle Eastern Countries for thousands of years and also in the treatment of diseases. Earlier studies have shown that N. sativa and its constituent thymoquinone (TQ) have important roles in the prevention and treatment of cancer by modulating cell signaling pathways. In this review, we summarize the role of N. sativa and its constituents TQ in the prevention of cancer through the activation or inactivation of molecular cell signaling pathways.

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“…Besides, previous studies categorized Thymoquinone between groups of ROS inducer plant-derived anti-cancer compounds [21]. It was also reported in p53-mutated Jurkat cells [22] in prostate cancer cell [23], in lymphoma cell line [24], in acute lymphocyte leukemia cell line [25], in pancreatic cancer cells [2] and in colorectal cancer cells [11].…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Study on Similarity Effect of Thymoquinone on Epithelial Breast Cancer and Colorectal Cancer Cell Lines

Motaghed¹

2015

AJLS

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Abstract:Adenocarcinoma is known as a common type of cancer which includes 85% of breast carcinoma and 95% of colorectal carcinoma. Up until now, the cytotoxic effect of Thymoquinone on different types of tumor cells has been reported. It was hypothesized that Thymoquinone has similar effect on cancer arising from epithelial cells through gene expression analysis in MCF7 breast cancer and HT-29 colon cancer cell lines. The quantity and quality of RNA samples were identified using RNeasyPlus Mini kit and RNA 6000 Nano LabChip kit, respectively. The purified RNA samples of MCF7 cells were used in two-color 8×60K cDNA array platform with SurePrint Agilent technology. The hybridized cRNA/cDNA probes were identified due to labeling with red and green cyanine dyes using GE X hybridization buffer HI-RPM. LOWESS normalization reduced the dye bias on the array slide using feature extraction software. Gene ontology analysis was done after performing different steps of filtering to reduce not satisfied genes using Gene Spring software. Two-step RT-qPCR assay using Taq Man fast advanced master mix analyzed the most up and down regulated genes in MCF7 compared to HT-29 cancer cell lines. The 10 different cancer cell lines in a form of universal reference RNA was used as standard data set comparison and as a positive control RNA in cDNA array and RT-qPCR assay, respectively. The T-test statistical analysis of independent samples showed that there is no significant difference between two types of cell lines due to Thymoquinone treatment with p-value 0.844. Among the selected genes; CARD16, UGT1A8, SLC7A11, IFIT1, IF16 and IFIT3 were expressed significantly (0.009, 0.0001, 0.037, 0.098, 0.0001 and 0.033, respectively) in breast cancer compare to colon cancer cells. The findings indicated similar effect of Thymoquinone on cancer arising from epithelial cells.

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Thymoquinone suppresses growth and induces apoptosis via generation of reactive oxygen species in primary effusion lymphoma

Cited by 90 publications

References 47 publications

Thymoquinone chemosensitizes colon cancer cells through inhibition of NF-κB

Thymoquinone chemosensitizes colon cancer cells through inhibition of NF-κB

Therapeutic Implications of Black Seed and Its Constituent Thymoquinone in the Prevention of Cancer through Inactivation and Activation of Molecular Pathways

Gene Expression Study on Similarity Effect of Thymoquinone on Epithelial Breast Cancer and Colorectal Cancer Cell Lines

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