Thymoquinone inhibits inflammation, neoangiogenesis and vascular remodeling in asthma mice

Su, Xinming; Ren, Yuan; Yu, Na; Kong, Lingfei; Kang, Jing

doi:10.1016/j.intimp.2016.05.018

Cited by 36 publications

(30 citation statements)

References 54 publications

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“…In our study, both TQ and piperine worked synergistically to inhibit angiogenesis by lowering VEGF levels. These results agree with the recent findings that showed the ability of TQ and piperine to inhibit angiogenesis [ 27 , 28 ]. However, angiogenesis inhibition as a single target cannot cause complete tumor regression [ 29 ].…”

Section: Discussionsupporting

confidence: 93%

Regressions of Breast Carcinoma Syngraft Following Treatment with Piperine in Combination with Thymoquinone

Talib

2017

Sci. Pharm.

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Thymoquinone (TQ) and piperine, the active ingredients in cumin (Nigella sativa) and black pepper (Piper longum), respectively, exhibit various bioactivities including anticancer effects. The aim of the present study is to investigate the antineoplastic activity of a combination of TQ and piperine against breast cancer implanted in mice. The antiproliferative effects of TQ, piperine, and a combination of both agents were tested against mouse epithelial breast cancer cell line (EMT6/P) using MTT assay. The isobolographic method was used to calculate the combination index (CI). Degree of angiogenesis inhibition was detected by measuring vascular endothelial growth factor (VEGF) levels in tissue culture for all treatments. EMT6/P cells were inoculated in Balb/C mice and the antitumor effect of TQ, piperine, and their combination was assessed. Changes in tumor size were calculated for all treatments. Tumor histology was examined using the hematoxylin/eosin staining protocol. Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) colorimetric assay and caspase-3 activity assays were used to detect apoptosis. Serum levels of interferon (INF)-γ, interleukin (IL)-4, IL-2, and IL-10 were measured using ELISA and treatment toxicity was evaluated by measuring serum levels of aspartate transaminase (AST), alanine transaminase (ALT), and creatinine. A clear synergistic antiproliferative interaction between TQ and piperine was observed with CI value of 0.788. The combination therapy resulted in significant reduction in tumor size with percentage cure of 60% and percentage death of 0%. High degrees of apoptosis and geographical necrosis were induced in tumors treated with the combination therapy. Combination therapy caused significant decrease in VEGF expression and increased serum INF-γ levels. Normal serum levels of AST, ALT, and creatinine were observed in tumor-bearing mice treated with the combination therapy. The combination of TQ and piperine acts synergistically to target breast cancer in vitro and in vivo. This novel combination exerts its effect by angiogenesis inhibition, apoptosis induction, and shifting the immune response toward T helper1 response. This combination therapy deserves further investigation (including measurement of hypoxia-inducible factor (HIF)1α to be used in clinical studies.

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Section: Discussionsupporting

confidence: 93%

Regressions of Breast Carcinoma Syngraft Following Treatment with Piperine in Combination with Thymoquinone

Talib

2017

Sci. Pharm.

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“…The phosphorylation of Akt at S473 plays a vital role in essential cellular by phosphorylating several downstream substrates. Several studies have shown the association between PI3K/ AKT signaling pathway and TQ-mediated inhibition of different cell types growth (Khan et al, 2017;Su et al, 2016;Dirican et al, 2015). As shown in Figure 3a and b, simultaneous downregulation and upregulation of pAkt and PTEN were noticed in dose dependently manner, and showed most effective with concomitant exposure of TQ (20 mM) and cyclo (p < 0.001) against vehicle control and cyclo alone as well.…”

Section: Tq Induced the Inhibition Of Akt Phosphorylation Alters Akt/mentioning

confidence: 76%

Thymoquinone Augments Cyclophosphamide-Mediated Inhibition of Cell Proliferation in Breast Cancer Cells

Khan

Aldebasy

Alsuhaibani

et al. 2019

Asian Pac J Cancer Prev

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“…A recent report has depicted that this bioactive component inhibited IL-1β-induced inflammation via downregulating NF-κB and MAPKs signaling in human osteoarthritis chondrocytes [ 130 ]. It also prevented inflammation, neoangiogenesis, and vascular remodeling in asthma in vivo [ 131 ]. Thymoquinone also inhibited TNF-α-induced inflammation and cell adhesion in RA, thus making it a promising anti-inflammatory agent [ 132 ].…”

Section: Active Components Of Spices Inflammatory Pathways and Chromentioning

confidence: 99%

Chronic diseases, inflammation, and spices: how are they linked?

et al. 2018

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Extensive research within the last several decades has revealed that the major risk factors for most chronic diseases are infections, obesity, alcohol, tobacco, radiation, environmental pollutants, and diet. It is now well established that these factors induce chronic diseases through induction of inflammation. However, inflammation could be either acute or chronic. Acute inflammation persists for a short duration and is the host defense against infections and allergens, whereas the chronic inflammation persists for a long time and leads to many chronic diseases including cancer, cardiovascular diseases, neurodegenerative diseases, respiratory diseases, etc. Numerous lines of evidence suggest that the aforementioned risk factors induced cancer through chronic inflammation. First, transcription factors NF-κB and STAT3 that regulate expression of inflammatory gene products, have been found to be constitutively active in most cancers; second, chronic inflammation such as pancreatitis, prostatitis, hepatitis etc. leads to cancers; third, activation of NF-κB and STAT3 leads to cancer cell proliferation, survival, invasion, angiogenesis and metastasis; fourth, activation of NF-κB and STAT3 leads to resistance to chemotherapy and radiation, and hypoxia and acidic conditions activate these transcription factors. Therefore, targeting these pathways may provide opportunities for both prevention and treatment of cancer and other chronic diseases. We will discuss in this review the potential of various dietary agents such as spices and its components in the suppression of inflammatory pathways and their roles in the prevention and therapy of cancer and other chronic diseases. In fact, epidemiological studies do indicate that cancer incidence in countries such as India where spices are consumed daily is much lower (94/100,000) than those where spices are not consumed such as United States (318/100,000), suggesting the potential role of spices in cancer prevention.

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Thymoquinone inhibits inflammation, neoangiogenesis and vascular remodeling in asthma mice

Cited by 36 publications

References 54 publications

Regressions of Breast Carcinoma Syngraft Following Treatment with Piperine in Combination with Thymoquinone

Regressions of Breast Carcinoma Syngraft Following Treatment with Piperine in Combination with Thymoquinone

Thymoquinone Augments Cyclophosphamide-Mediated Inhibition of Cell Proliferation in Breast Cancer Cells

Chronic diseases, inflammation, and spices: how are they linked?

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