Thymoquinone inhibits biofilm formation and virulence properties of periodontal bacteria

Tantivitayakul, Pornpen; Kaypetch, Rattiporn; Muadchiengka, T.

doi:10.1016/j.archoralbio.2020.104744

Cited by 16 publications

(8 citation statements)

References 47 publications

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“…It has the ability to prevent biofilm formation and the hemolytic action of these bacteria. Thus, TQ is promising in terms of being developed and utilized for periodontal treatment, specifically for preventing periodontitis progression [ 34 ]. In this study, the bone-healing parameters in the TQ-containing groups were better than those in the other groups.…”

Section: Discussionmentioning

confidence: 99%

The effect of local application of thymoquinone, Nigella sativa’s bioactive component, on bone healing in experimental bone defects infected with Porphyromonas gingivalis

Baştuğ

Tomruk

Güzel

et al. 2022

J Periodontal Implant Sci

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Purpose This study was performed to evaluate the influence of local application of thymoquinone (TQ) on bone healing in experimental bone defects infected with Porphyromonas gingivalis (PG). Methods Forty-two female rats were randomly divided into 6 groups. A bone defect was created on the right tibia of all animals. The PG, PG/collagen membrane (COL) and PG/TQ/COL groups were infected with PG. In the COL and PG/COL groups, the defects were covered with a COL; in the TQ/COL and PG/TQ/COL groups, the defects were covered with a TQ-containing COL. After 28 days, all animals were sacrificed. Quantitative measurements of new bone formation and osteoblast lining, as well as semiquantitative measurements of capillary density and tissue response, were analyzed. Furthermore, the presence of bacterial infections in defect areas was evaluated. Results The new bone formation, osteoblast number, and capillary density were significantly higher in the TQ groups than in the control groups ( P <0.001, P <0.001, and P <0.01, respectively). In a comparison between the TQ/COL group, with a TQ-containing COL (TQ/COL), and the PG – infected TQ-containing COL (PG/TQ/COL) group, the newly formed bone and capillary density were higher in the TQ/COL group ( P <0.01). When the control group was compared to the PG, PG/COL, and PG/TQ/COL groups in terms of tissue response, the differences were statistically significant ( P <0.001, P =0.02, and P =0.041, respectively). The intensity of the inflammatory cell reaction was higher in the PG, PG/COL, and PG/TQ/COL groups ( P <0.05). Conclusions Within the limitations of this study, the local application of a TQ-containing COL positively affected bone healing even if the bone defects were infected. The results suggest that TQ increased angiogenesis and showed promise for accelerating bone defect healing. Further research is warranted to support these findings and reach more definitive conclusions.

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Section: Discussionmentioning

confidence: 99%

The effect of local application of thymoquinone, Nigella sativa’s bioactive component, on bone healing in experimental bone defects infected with Porphyromonas gingivalis

Baştuğ

Tomruk

Güzel

et al. 2022

J Periodontal Implant Sci

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“…Similarly, the green tea extract, at a dosage of 75 mg/mL, demonstrated more than 50% reduction in gene expression of Rgp ( Fournier-Larente et al., 2016 ). Recently, a plant derived monoterpene from black cumin, thymoquinone, was found to downregulate the gene expression of Rgp which eventually led to the inhibition of biofilm formation and hemolytic activity in P. gingivalis , thereby reducing its virulency ( Tantivitayakul et al., 2020 ).…”

Section: Gingipain Rmentioning

confidence: 99%

“…Besides, production of outer membrane vesicles (OMV) that serve to transport molecular effector related to host immune dysregulation is also associated with gingipains ( Zhang et al., 2016 ; Okamura et al., 2021 ). Inhibition of the gingipains is shown to reduce hemolytic activities and biofilm formation of P. gingivalis , thus limiting its virulency ( Tantivitayakul et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Implications of Porphyromonas gingivalis peptidyl arginine deiminase and gingipain R in human health and diseases

Chow

Yam

Gunasekaran

et al. 2022

Front. Cell. Infect. Microbiol.

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Porphyromonas gingivalis is a major pathogenic bacterium involved in the pathogenesis of periodontitis. Citrullination has been reported as the underlying mechanism of the pathogenesis, which relies on the interplay between two virulence factors of the bacterium, namely gingipain R and the bacterial peptidyl arginine deiminase. Gingipain R cleaves host proteins to expose the C-terminal arginines for peptidyl arginine deiminase to citrullinate and generate citrullinated proteins. Apart from carrying out citrullination in the periodontium, the bacterium is found capable of citrullinating proteins present in the host synovial tissues, atherosclerotic plaques and neurons. Studies have suggested that both virulence factors are the key factors that trigger distal effects mediated by citrullination, leading to the development of some non-communicable diseases, such as rheumatoid arthritis, atherosclerosis, and Alzheimer’s disease. Thus, inhibition of these virulence factors not only can mitigate periodontitis, but also can provide new therapeutic solutions for systematic diseases involving bacterial citrullination. Herein, we described both these proteins in terms of their unique structural conformations and biological relevance to different human diseases. Moreover, investigations of inhibitory actions on the enzymes are also enumerated. New approaches for identifying inhibitors for peptidyl arginine deiminase through drug repurposing and virtual screening are also discussed.

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“…There is strong association between AD and periodontitis which is a chronic infectious and inflammatory disease characterized by irreversible attachment loss of involved teeth [ 4 ]. Porphyromonas gingivalis (Pg) is a keystone pathogen in periodontitis [ 5 ] and is also a significant risk factor for various systemic diseases including rheumatoid arthritis [ 6 , 7 ], ischemic stroke [ 8 ] and AD [ 9 ]. In the case of AD, periodontitis is associated with more severe cognitive impairment [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Exogenous monocyte myeloid-derived suppressor cells ameliorate immune imbalance, neuroinflammation and cognitive impairment in 5xFAD mice infected with Porphyromonas gingivalis

Xiao

Lin

et al. 2023

J Neuroinflammation

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Background Periodontitis is closely associated with the pathogenesis of Alzheimer’s disease (AD). Porphyromonas gingivalis (Pg), the keystone periodontal pathogen, has been reported in our recent study to cause immune-overreaction and induce cognitive impairment. Monocytic myeloid-derived suppressor cells (mMDSCs) possess potent immunosuppressive function. It is unclear whether mMDSCs-mediated immune homeostasis is impaired in AD patients with periodontitis, and whether exogenous mMDSCs could ameliorate immune-overreaction and cognitive impairment induced by Pg. Methods To explore the influence of Pg on cognitive function, neuropathology and immune balance in vivo, 5xFAD mice were treated with live Pg by oral gavage, three times a week for 1 month. The cells of peripheral blood, spleen and bone marrow from 5xFAD mice were treated with Pg to detect the proportional and functional alterations of mMDSCs in vitro. Next, exogenous mMDSCs were sorted from wild-type healthy mice and intravenously injected into 5xFAD mice that were infected with Pg. We used behavioral tests, flow cytometry and immunofluorescent staining to evaluate whether exogenous mMDSCs could ameliorate the cognitive function, immune homeostasis and reduce neuropathology exacerbated by Pg infection. Results Pg exacerbated cognitive impairment in 5xFAD mice, with the deposition of amyloid plaque and increased number of microglia in the hippocampus and cortex region. The proportion of mMDSCs decreased in Pg-treated mice. In addition, Pg reduced the proportion and the immunosuppressive function of mMDSCs in vitro. Supplement of exogenous mMDSCs improved the cognitive function, and enhanced the proportions of mMDSCs and IL-10+ T cells of 5xFAD mice infected with Pg. At the same time, supplement of exogenous mMDSCs increased the immunosuppressive function of endogenous mMDSCs while decreased the proportions of IL-6+ T cells and IFN-γ+ CD4+ T cells. In addition, the deposition of amyloid plaque decreased while the number of neurons increased in the hippocampus and cortex region after the supplement of exogenous mMDSCs. Furthermore, the number of microglia increased with an increase in the proportion of M2 phenotype. Conclusions Pg can reduce the proportion of mMDSCs, induce immune-overreaction, and exacerbate the neuroinflammation and cognitive impairment in 5xFAD mice. Supplement of exogenous mMDSCs can reduce the neuroinflammation, immune imbalance and cognitive impairment in 5xFAD mice infected with Pg. These findings indicate the mechanism of AD pathogenesis and Pg-mediated promotion of AD, and provide a potential therapeutic strategy for AD patients.

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Thymoquinone inhibits biofilm formation and virulence properties of periodontal bacteria

Cited by 16 publications

References 47 publications

The effect of local application of thymoquinone, Nigella sativa’s bioactive component, on bone healing in experimental bone defects infected with Porphyromonas gingivalis

The effect of local application of thymoquinone, Nigella sativa’s bioactive component, on bone healing in experimental bone defects infected with Porphyromonas gingivalis

Implications of Porphyromonas gingivalis peptidyl arginine deiminase and gingipain R in human health and diseases

Exogenous monocyte myeloid-derived suppressor cells ameliorate immune imbalance, neuroinflammation and cognitive impairment in 5xFAD mice infected with Porphyromonas gingivalis

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